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View article: Capillary Gel Electrophoresis of Proteins: Historical overview and recent advances
Capillary Gel Electrophoresis of Proteins: Historical overview and recent advances Open
This review summarizes the fundamental principles, basic methodologies, strength and weaknesses of capillary gel electrophoresis of proteins by providing both a short historical overview and highlighting new developments and applications i…
View article: Stability improvement of laccase for micropollutant removal of pharmaceutical origins from municipal wastewater
Stability improvement of laccase for micropollutant removal of pharmaceutical origins from municipal wastewater Open
Micropollutants are persistent and hazardous materials in low concentrations (ng L −1 –μg L −1 ), including substances such as pharmaceuticals, personal care products and industrial chemicals. The advancement of analytical chemistry has al…
View article: Stability improvement of laccase for micropollutant removal of pharmaceutical origins from municipal wastewater
Stability improvement of laccase for micropollutant removal of pharmaceutical origins from municipal wastewater Open
Micropollutants are persistent and hazardous materials in low concentrations (ng·L− 1 – µg·L− 1), including substances such as pharmaceuticals, personal care products and industrial chemicals. The advancement of analytical chemistry has al…
View article: Glycosimilarity assessment of biotherapeutics 1: Quantitative comparison of the N -glycosylation of the innovator and a biosimilar version of etanercept
Glycosimilarity assessment of biotherapeutics 1: Quantitative comparison of the N -glycosylation of the innovator and a biosimilar version of etanercept Open
The carbohydrate moieties on the polypeptide chains in most glycoprotein based biotherapeutics and their biosimilars play essential roles in such major mechanisms of actions as antibody-dependent cell-mediated cytotoxicity, complement-depe…
View article: Quantitative characterization of plasma treated PDMS microfluidic substrates by inverse gas chromatography
Quantitative characterization of plasma treated PDMS microfluidic substrates by inverse gas chromatography Open
The effect of air plasma exposure time on the surface energies and acid-base characteristics of polydimethylsiloxane (PDMS) particles was studied. Polymerized PDMS powder was radio frequency induced air plasma irradiated for 2–10 s with th…
View article: Continuous-Flow-Based Microfluidic Systems for Therapeutic Monoclonal Antibody Production and Organ-on-a-Chip Drug Testing
Continuous-Flow-Based Microfluidic Systems for Therapeutic Monoclonal Antibody Production and Organ-on-a-Chip Drug Testing Open
Continuous-flow processing in the manufacturing of modern biotherapeutics represents a great potential and could significantly improve productivity and product quality as well as reduce operating costs. Microfluidic perfusion systems are n…
View article: Recent advances in column coatings for capillary electrophoresis of proteins
Recent advances in column coatings for capillary electrophoresis of proteins Open
Capillary coatings effectively improve the separation performance of proteins in capillary electrophoresis, mainly by reducing protein adsorption onto the inner capillary wall and by regulating the electroosmotic flow (EOF) to accommodate …
View article: A fully automated linear polyacrylamide coating and regeneration method for capillary electrophoresis of proteins
A fully automated linear polyacrylamide coating and regeneration method for capillary electrophoresis of proteins Open
Surface modification of the inner capillary wall in CE of proteins is frequently required to alter EOF and to prevent protein adsorption. Manual protocols for such coating techniques are cumbersome. In this paper, an automated covalent lin…