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View article: CRISPR/Cas-mediated polyphenol oxidase gene knockout in potato reveals divergent roles in resistance to bacterial wilt and late blight
CRISPR/Cas-mediated polyphenol oxidase gene knockout in potato reveals divergent roles in resistance to bacterial wilt and late blight Open
Polyphenol oxidases (PPOs) play a pivotal role in plant immune responses by catalysing the oxidation of phenolic compounds into cytotoxic quinones and melanin and contributing to the fortification of cell walls. Despite their biological si…
View article: Root-based inorganic carbon uptake increases the growth of Arabidopsis thaliana and changes transporter expression and nitrogen and sulfur metabolism
Root-based inorganic carbon uptake increases the growth of Arabidopsis thaliana and changes transporter expression and nitrogen and sulfur metabolism Open
Root-based uptake of inorganic carbon has been suggested as an additional carbon source. Our study aimed to characterize and understand the root-based uptake and fixation mechanisms and their impact on plant growth. 13 C-labeled bicarbonat…
View article: Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids
Plasticity of parental CENH3 incorporation into the centromeres in wheat × barley F1 hybrids Open
Incorporating the centromere-specific histone H3 protein CENH3 into the centromeric nucleosomes is indispensable for accurate centromere function and balanced chromosome segregation in most eukaryotes, including higher plants. In the cell …
View article: Heat stress during male meiosis impairs cytoskeletal organization, spindle assembly and tapetum degeneration in wheat
Heat stress during male meiosis impairs cytoskeletal organization, spindle assembly and tapetum degeneration in wheat Open
The significance of heat stress in agriculture is ever-increasing with the progress of global climate changes. Due to a negative effect on the yield of staple crops, including wheat, the impairment of plant reproductive development trigger…
View article: Targeted mutations in the GW2.1 gene modulate grain traits and induce yield loss in barley
Targeted mutations in the GW2.1 gene modulate grain traits and induce yield loss in barley Open
Grain Width and Weight 2 (GW2) is an E3-ubiquitin ligase-encoding gene that negatively regulates the size and weight of the grain in cereal species. Therefore, disabling GW2 gene activity was suggested for enhancing crop productivity. We s…
View article: Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH
Rapid in-solution preparation of somatic and meiotic plant cell nuclei for high-quality 3D immunoFISH and immunoFISH-GISH Open
Background Though multicolour labelling methods allow the routine detection of a wide range of fluorescent (immuno)probe types in molecular cytogenetics, combined applications for the simultaneous in situ detection of proteins and nucleic …
View article: High-throughput ligand profile characterization in novel biosensor cell lines expressing seven heterologous olfactory receptors for the detection of volatile plant biomarkers
High-throughput ligand profile characterization in novel biosensor cell lines expressing seven heterologous olfactory receptors for the detection of volatile plant biomarkers Open
Agriculturally important crop plants emit a multitude of volatile organic compounds (VOCs), which are excellent indicators of their health status and/or their interaction with pathogens and pests. Here, we present the generation of a novel…
View article: Global transcriptome and targeted metabolite analyses of roots reveal different defence mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars
Global transcriptome and targeted metabolite analyses of roots reveal different defence mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars Open
Ralstonia solanacearum ( Rs ), the causal agent of bacterial wilt disease in an unusually wide range of host plants, including potato ( Solanum tuberosum ), is one of the most destructive phytopathogens that seriously reduces crop yields w…
View article: bZIP transcription factors repress the expression of wheat (Triticum aestivum L.) high molecular weight glutenin subunit genes in vegetative tissues
bZIP transcription factors repress the expression of wheat (Triticum aestivum L.) high molecular weight glutenin subunit genes in vegetative tissues Open
High molecular weight glutenin subunits (HMW GS) represent an important fraction of endosperm-specific seed-storage proteins that provide elasticity to bread dough. Previously, the second cis -regulatory module (CRM2) was found to be one o…
View article: Alkaline Salt Tolerance of the Biomass Plant Arundo donax
Alkaline Salt Tolerance of the Biomass Plant Arundo donax Open
Soil alkalinization and salinization have increased worldwide due to extreme and/or prolonged drought periods as well as insufficient irrigation. Since crops generally react to soil salinity and high pH with decreased yield, the cultivatio…
View article: Emission of novel volatile biomarkers for wheat powdery mildew
Emission of novel volatile biomarkers for wheat powdery mildew Open
Natural ecosystems including host-pathogen interactions produce a plethora of biogenic volatile organic compounds (BVOCs). Infections by phytopathogens change the volatile profile substantially and dynamically over a crop field or even on …
View article: Genetic Alterations That Do or Do Not Occur Naturally; Consequences for Genome Edited Organisms in the Context of Regulatory Oversight
Genetic Alterations That Do or Do Not Occur Naturally; Consequences for Genome Edited Organisms in the Context of Regulatory Oversight Open
The ability to successfully exploit genome edited organisms for the benefit of food security and the environment will essentially be determined by the extent to which these organisms fall under specific regulatory provisions. In many juris…