Lata Govada
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View article: Inhibition of IRAK4 by microbial trimethylamine blunts metabolic inflammation and ameliorates glycemic control
Inhibition of IRAK4 by microbial trimethylamine blunts metabolic inflammation and ameliorates glycemic control Open
The global type 2 diabetes epidemic is a major health crisis. Although the microbiome has roles in the onset of insulin resistance (IR), low-grade inflammation and diabetes, the microbial compounds controlling these processes remain to be …
View article: Experimental localization of metal-binding sites reveals the role of metal ions in type II DNA topoisomerases
Experimental localization of metal-binding sites reveals the role of metal ions in type II DNA topoisomerases Open
Metal ions have important roles in supporting the catalytic activity of DNA-regulating enzymes such as topoisomerases (topos). Bacterial type II topos, gyrases and topo IV, are primary drug targets for fluoroquinolones, a class of clinical…
View article: Protein Crystals Nucleated and Grown by Means of Porous Materials Display Improved X-ray Diffraction Quality
Protein Crystals Nucleated and Grown by Means of Porous Materials Display Improved X-ray Diffraction Quality Open
Well-diffracting protein crystals are indispensable for X-ray diffraction analysis, which is still the most powerful method for structure-function studies of biomolecules. A promising approach to growing such crystals is the use of porous …
View article: Graphene‐Based Nucleants for Protein Crystallization
Graphene‐Based Nucleants for Protein Crystallization Open
Protein crystallization remains a major bottleneck for the determination of high resolution structures. Nucleants can accelerate the process but should ideally be compatible with high throughput robotic screening. Polyethylene glycol graft…
View article: Why Do Protein Crystals Grown With the Aid of Porous Materials Show Better Diffraction Quality?
Why Do Protein Crystals Grown With the Aid of Porous Materials Show Better Diffraction Quality? Open
Well-diffracting protein crystals are indispensable for X-ray diffraction analysis, which is still the most powerful method for structure-function studies of biomolecules. A promising approach to growing such crystals is by using porous nu…
View article: X-ray crystallographic studies of RoAb13 bound to PIYDIN, a part of the N terminal domain of C-C chemokine receptor 5: Sample 7 diffraction images
X-ray crystallographic studies of RoAb13 bound to PIYDIN, a part of the N terminal domain of C-C chemokine receptor 5: Sample 7 diffraction images Open
Short peptide (PIYDIN) Sample 7 diffraction images for 7nw3. Publication:- Govada, L., Saridakis, E., Kassen, S.C., Bin-Ramzi, A., Morgan, R.M., Chain, B., Helliwell, J.R. & Chayen, N.E. (2021). IUCrJ 8, https://doi.org/10.1107/S2052252521…
View article: X-ray crystallographic studies of RoAb13 bound to PIYDIN, a part of the Nterminal domain of C-C chemokine receptor 5: Long peptide Sample 4 diffraction images
X-ray crystallographic studies of RoAb13 bound to PIYDIN, a part of the Nterminal domain of C-C chemokine receptor 5: Long peptide Sample 4 diffraction images Open
Long peptide Sample 4 diffraction images for 7njz. Publication:- Govada, L., Saridakis, E., Kassen, S.C., Bin-Ramzi, A., Morgan, R.M., Chain, B., Helliwell, J.R. & Chayen, N.E. (2021). IUCrJ 8, https://doi.org/10.1107/S2052252521005340. Ar…
View article: X-ray crystallographic studies of RoAb13 bound to PIYDIN, a part of the N terminal domain of C-C chemokine receptor 5: Sample 7 diffraction images
X-ray crystallographic studies of RoAb13 bound to PIYDIN, a part of the N terminal domain of C-C chemokine receptor 5: Sample 7 diffraction images Open
Short peptide (PIYDIN) Sample 7 diffraction images for 7nw3. Publication:- Govada, L., Saridakis, E., Kassen, S.C., Bin-Ramzi, A., Morgan, R.M., Chain, B., Helliwell, J.R. & Chayen, N.E. (2021). IUCrJ 8, https://doi.org/10.1107/S2052252521…
View article: X-ray crystallographic studies of RoAb13 bound to PIYDIN, a part of the N-terminal domain of C-C chemokine receptor 5
X-ray crystallographic studies of RoAb13 bound to PIYDIN, a part of the N-terminal domain of C-C chemokine receptor 5 Open
C-C chemokine receptor 5 (CCR5) is a major co-receptor molecule used by HIV-1 to enter cells. This led to the hypothesis that stimulating an antibody response would block HIV with minimal toxicity. Here, X-ray crystallographic studies of t…
View article: Theoretical and experimental investigation of protein crystal nucleation in pores and crevices
Theoretical and experimental investigation of protein crystal nucleation in pores and crevices Open
The nucleation ability of pores is explained using the equilibration between the cohesive energy maintaining the integrity of a crystalline cluster and the destructive energy tending to tear it up. It is shown that to get 3D crystals it is…
View article: Analysis of Glulisine Crystallisation Utilising Phase Diagrams and Nucleants
Analysis of Glulisine Crystallisation Utilising Phase Diagrams and Nucleants Open
Glulisine is a US Food and Drug Administration (FDA) approved insulin analogue, used for controlling hyperglycaemia in patients with diabetes mellitus (DM). It is fast acting which better approximates physiological insulin secretion, impro…
View article: Choosing the Method of Crystallization to Obtain Optimal Results
Choosing the Method of Crystallization to Obtain Optimal Results Open
Anyone who has ever attempted to crystallise a protein or other biological macromolecule has encountered at least one, if not all of the following scenarios: No crystals at all, tiny low quality crystals; phase separation; amorphous precip…
View article: Chlamydia protein Pgp3 studied at high resolution in a new crystal form
Chlamydia protein Pgp3 studied at high resolution in a new crystal form Open
The protein Pgp3 is implicated in the sexually transmitted disease chlamydia and comprises an extended complex arrangement of a C-terminal domain (CTD) and an N-terminal domain (NTD) linked by a triple-helix coiled coil (THCC). Here, the X…
View article: Inhibition of IRAK4 by microbial trimethylamine blunts metabolic inflammation and ameliorates glycemic control
Inhibition of IRAK4 by microbial trimethylamine blunts metabolic inflammation and ameliorates glycemic control Open
The global type 2 diabetes epidemic is a major health crisis and there is a critical need for innovative strategies to fight it. Although the microbiome plays important roles in the onset of insulin resistance (IR) and low-grade inflammati…
View article: Enhancing the success of crystallization: strategies and techniques
Enhancing the success of crystallization: strategies and techniques Open
The past 15 years have seen remarkable advances in the miniaturisation, automation and analysis of crystallisation experiments.However, production of high quality crystals persistently remains a major hurdle to structure determination; it …
View article: Smart materials for increasing the success of protein crystallization
Smart materials for increasing the success of protein crystallization Open
A new class of nucleants has emerged with the discovery that porous materials with a distribution of pore sizes similar to those of protein molecules are effective for inducing protein crystals.Building on the success of such porous materi…
View article: Reductively PEGylated carbon nanomaterials and their use to nucleate 3D protein crystals: a comparison of dimensionality
Reductively PEGylated carbon nanomaterials and their use to nucleate 3D protein crystals: a comparison of dimensionality Open
Reductive grafting with mPEG is effective on a wide range of carbon nanomaterials. However, 2D forms are most effective as protein nucleants.
View article: A Linear Epitope in the N-Terminal Domain of CCR5 and Its Interaction with Antibody
A Linear Epitope in the N-Terminal Domain of CCR5 and Its Interaction with Antibody Open
The CCR5 receptor plays a role in several key physiological and pathological processes and is an important therapeutic target. Inhibition of the CCR5 axis by passive or active immunisation offers one very selective strategy for interventio…
View article: Automating the application of smart materials for protein crystallization
Automating the application of smart materials for protein crystallization Open
The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imp…