Laura Klenow
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View article: Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis
Protocol for identifying immune mediators during Leishmania infection in mice using metabolomic analysis Open
View article: Monoclonal antibodies targeting sites in respiratory syncytial virus attachment G protein provide protection against RSV-A and RSV-B in mice
Monoclonal antibodies targeting sites in respiratory syncytial virus attachment G protein provide protection against RSV-A and RSV-B in mice Open
View article: Production of leishmanin skin test antigen from Leishmania donovani for future reintroduction in the field
Production of leishmanin skin test antigen from Leishmania donovani for future reintroduction in the field Open
The leishmanin skin test was used for almost a century to detect exposure and immunity to Leishmania , the causative agent of leishmaniasis, a major neglected tropical disease. Due to a lack of antigen used for the intradermal injection, t…
View article: Inactivated influenza virions are a flexible vaccine platform for eliciting protective antibody responses against neuraminidase
Inactivated influenza virions are a flexible vaccine platform for eliciting protective antibody responses against neuraminidase Open
Most seasonal influenza vaccines are produced using hemagglutinin (HA) surface antigens from inactivated virions. However, virions are thought to be a suboptimal source for the less abundant neuraminidase (NA) surface antigen, which is als…
View article: Modernizing the original influenza vaccine to enhance protective antibody responses against neuraminidase antigens
Modernizing the original influenza vaccine to enhance protective antibody responses against neuraminidase antigens Open
Most seasonal influenza vaccines are produced using hemagglutinin (HA) surface antigens from inactivated virions. However, virions are thought to be a suboptimal source for the less abundant NA surface antigen, which is also protective aga…
View article: Silencing Transcription from an Influenza Reverse Genetics Plasmid in <i>E. coli</i> Enhances Gene Stability
Silencing Transcription from an Influenza Reverse Genetics Plasmid in <i>E. coli</i> Enhances Gene Stability Open
Reverse genetics (RG) systems have been instrumental for determining the molecular aspects of viral replication, pathogenesis, and for the development of therapeutics. Here, we demonstrate that genes encoding the influenza surface antigens…
View article: Influenza virus and pneumococcal neuraminidases enhance catalysis by similar yet distinct sialic acid–binding strategies
Influenza virus and pneumococcal neuraminidases enhance catalysis by similar yet distinct sialic acid–binding strategies Open
View article: Sialosides Containing 7-<i>N</i>-Acetyl Sialic Acid Are Selective Substrates for Neuraminidases from Influenza A Viruses
Sialosides Containing 7-<i>N</i>-Acetyl Sialic Acid Are Selective Substrates for Neuraminidases from Influenza A Viruses Open
Sialidases or neuraminidases are sialic-acid-cleaving enzymes that are expressed by a broad spectrum of organisms, including pathogens. In nature, sialic acids are monosaccharides with diverse structural variations, but the lack of novel p…
View article: Antigenic comparison of the neuraminidases from recent influenza A vaccine viruses and 2019–2020 circulating strains
Antigenic comparison of the neuraminidases from recent influenza A vaccine viruses and 2019–2020 circulating strains Open
Although viral-based influenza vaccines contain neuraminidase (NA or N) antigens from the recommended seasonal strains, NA is not extensively evaluated like hemagglutinin (H) during the strain selection process. Here, we compared the antig…
View article: Protective antigenic sites identified in respiratory syncytial virus fusion protein reveals importance of p27 domain
Protective antigenic sites identified in respiratory syncytial virus fusion protein reveals importance of p27 domain Open
View article: Design of the Recombinant Influenza Neuraminidase Antigen Is Crucial for Its Biochemical Properties and Protective Efficacy
Design of the Recombinant Influenza Neuraminidase Antigen Is Crucial for Its Biochemical Properties and Protective Efficacy Open
For several decades, it has been proposed that influenza vaccines could be supplemented with recombinant neuraminidase (rNA) to improve efficacy. However, some key questions for manufacturing stable and immunogenic rNAs remain to be answer…
View article: Design of the recombinant influenza neuraminidase antigen is crucial for protective efficacy
Design of the recombinant influenza neuraminidase antigen is crucial for protective efficacy Open
Supplementing influenza vaccines with recombinant neuraminidase (rNA) remains a promising approach for improving the suboptimal efficacy. However, correlations among rNA designs, properties, and protection have not been systematically inve…
View article: Balancing the influenza neuraminidase and hemagglutinin responses by exchanging the vaccine virus backbone
Balancing the influenza neuraminidase and hemagglutinin responses by exchanging the vaccine virus backbone Open
Virions are a common antigen source for many viral vaccines. One limitation to using virions is that the antigen abundance is determined by the content of each protein in the virus. This caveat especially applies to viral-based influenza v…
View article: Longitudinal antibody repertoire in “mild” versus “severe” COVID-19 patients reveals immune markers associated with disease severity and resolution
Longitudinal antibody repertoire in “mild” versus “severe” COVID-19 patients reveals immune markers associated with disease severity and resolution Open
SARS-CoV-2–induced human antibody repertoire in patients with COVID-19 reveals immune markers of disease severity and recovery.
View article: Antibody affinity maturation and plasma IgA associate with clinical outcome in hospitalized COVID-19 patients
Antibody affinity maturation and plasma IgA associate with clinical outcome in hospitalized COVID-19 patients Open
View article: Autoreactivity of Broadly Neutralizing Influenza Human Antibodies to Human Tissues and Human Proteins
Autoreactivity of Broadly Neutralizing Influenza Human Antibodies to Human Tissues and Human Proteins Open
Broadly neutralizing monoclonal antibodies (bNAbs) against conserved domains in the influenza hemagglutinin are in clinical trials. Several next generation influenza vaccines designed to elicit such bNAbs are also in clinical development. …
View article: Antibody signature induced by SARS-CoV-2 spike protein immunogens in rabbits
Antibody signature induced by SARS-CoV-2 spike protein immunogens in rabbits Open
Understanding antibody epitope diversity and affinity generated by SARS-CoV-2 spike antigens in rabbits can aid COVID-19 vaccine development.
View article: Antibody repertoire induced by SARS-CoV-2 spike protein immunogens
Antibody repertoire induced by SARS-CoV-2 spike protein immunogens Open
Multiple vaccine candidates against SARS-CoV-2 based on viral spike protein are under development. However, there is limited information on the quality of antibody response generated following vaccination by these vaccine modalities. To be…
View article: Human Antibody Repertoire following Ebola Virus Infection and Vaccination
Human Antibody Repertoire following Ebola Virus Infection and Vaccination Open
View article: Antibody-dependent enhancement of influenza disease promoted by increase in hemagglutinin stem flexibility and virus fusion kinetics
Antibody-dependent enhancement of influenza disease promoted by increase in hemagglutinin stem flexibility and virus fusion kinetics Open
Significance Next-generation influenza vaccines and broadly neutralizing antibodies (bNAbs) are in clinical development. Some of these do not block virus–receptor interactions and thus are predicted to provide protection via alternative me…
View article: Protective antigenic sites in respiratory syncytial virus G attachment protein outside the central conserved and cysteine noose domains
Protective antigenic sites in respiratory syncytial virus G attachment protein outside the central conserved and cysteine noose domains Open
Respiratory syncytial virus (RSV) is the major cause of lower respiratory tract disease in infants. Previously, we elucidated the antibody repertoire following primary RSV infection in infants. Whole genome-fragment phage display libraries…
View article: Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids
Cleavage and Structural Transitions during Maturation of Staphylococcus aureus Bacteriophage 80α and SaPI1 Capsids Open
In the tailed bacteriophages, DNA is packaged into spherical procapsids, leading to expansion into angular, thin-walled mature capsids. In many cases, this maturation is accompanied by cleavage of the major capsid protein (CP) and other ca…
View article: Competing scaffolding proteins determine capsid size during mobilization of Staphylococcus aureus pathogenicity islands
Competing scaffolding proteins determine capsid size during mobilization of Staphylococcus aureus pathogenicity islands Open
Staphylococcus aureus pathogenicity islands (SaPIs), such as SaPI1, exploit specific helper bacteriophages, like 80α, for their high frequency mobilization, a process termed ‘molecular piracy’. SaPI1 redirects the helper’s assembly pathway…
View article: Author response: Competing scaffolding proteins determine capsid size during mobilization of Staphylococcus aureus pathogenicity islands
Author response: Competing scaffolding proteins determine capsid size during mobilization of Staphylococcus aureus pathogenicity islands Open
View article: <i>Staphylococcus aureus</i> Pathogenicity Islands: Hijackers on the Bacteriophage Assembly Pathway.
<i>Staphylococcus aureus</i> Pathogenicity Islands: Hijackers on the Bacteriophage Assembly Pathway. Open
Journal Article Staphylococcus aureus Pathogenicity Islands: Hijackers on the Bacteriophage Assembly Pathway. Get access Terje Dokland, Terje Dokland Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, U.S.A Se…
View article: Preclinical evaluation of bacterially produced RSV-G protein vaccine: Strong protection against RSV challenge in cotton rat model
Preclinical evaluation of bacterially produced RSV-G protein vaccine: Strong protection against RSV challenge in cotton rat model Open
View article: Characterization of the Interactions between Staphylococcal Phage 80 Alpha Scaffold and Capsid Proteins
Characterization of the Interactions between Staphylococcal Phage 80 Alpha Scaffold and Capsid Proteins Open
Staphylococcal phage 80α can serve as a helper bacteriophage for a family of mobile genetic elements called Staphylococcus aureus pathogenicity islands (SaPIs). The prototype island, SaPI1, is able to hijack the 80α capsid assembly process…