Lauren B. Stadler
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View article: Seqwin: Ultrafast identification of signature sequences in microbial genomes
Seqwin: Ultrafast identification of signature sequences in microbial genomes Open
Motivation Polymerase chain reaction (PCR) enables rapid, cost-effective diagnostics but requires prior identification of genomic regions that allow sensitive and specific identification of target microbial groups, herein referred to as mi…
View article: A Nonlinear Hierarchical Time Series Approach to Citywide Trend Assessment of Viruses, Hot Spot Signals, and Right-Sizing System
A Nonlinear Hierarchical Time Series Approach to Citywide Trend Assessment of Viruses, Hot Spot Signals, and Right-Sizing System Open
Wastewater surveillance has proven to be a cost-effective cornerstone in public health, offering vital insights into a spectrum of community health issues, particularly during the COVID-19 pandemic. For many municipalities, multiple locati…
View article: Uncovering Dynamic Relationships Between SARS-CoV-2 Wastewater Concentrations and Community Infections via Bayesian Spatial Functional Concurrent Regression
Uncovering Dynamic Relationships Between SARS-CoV-2 Wastewater Concentrations and Community Infections via Bayesian Spatial Functional Concurrent Regression Open
Monitoring wastewater concentrations of SARS-CoV-2 yields a low-cost, noninvasive method for tracking disease prevalence and provides early warning signs of upcoming outbreaks in the serviced communities. There is tremendous clinical and p…
View article: Sensitivity, throughput, and cost analysis of concentration methods for multi-target pathogen wastewater monitoring
Sensitivity, throughput, and cost analysis of concentration methods for multi-target pathogen wastewater monitoring Open
Wastewater-based epidemiology is an efficient method for monitoring the transmission of diverse pathogens in communities. Standard wastewater surveillance workflows typically involve wastewater concentration, nucleic acid extraction, and p…
View article: Sequencing-Based Detection of Measles in Wastewater: Texas, January 2025
Sequencing-Based Detection of Measles in Wastewater: Texas, January 2025 Open
Measles is a potentially deadly viral infection spread via respiratory droplets from infected individuals. Outbreaks occur when vaccine coverage drops below the threshold of herd, or community, immunity. Using a sequencing-based approach, …
View article: Cross-order detection of bacteriophage transduction in communities using ribosomal RNA barcoding
Cross-order detection of bacteriophage transduction in communities using ribosomal RNA barcoding Open
Bacteriophages (phages) facilitate gene transfer and microbial evolution in all ecosystems and have applications as tools for engineering microbiomes and as antimicrobials. Historic efforts to map phage hosts, such as plaque assays, are li…
View article: Controlling the taxonomic composition of biological information storage in 16S ribosomal RNA
Controlling the taxonomic composition of biological information storage in 16S ribosomal RNA Open
Microbes can be programmed to record participation in gene transfer by coding biological-recording devices into mobile DNA. Upon DNA uptake, these devices transcribe a catalytic RNA (cat-RNA) that binds to conserved sequences within riboso…
View article: Plasmid Backbone Impacts Conjugation Rate, Transconjugant Fitness, and Community Assembly of Genetically Bioaugmented Soil Microbes for PAH Bioremediation
Plasmid Backbone Impacts Conjugation Rate, Transconjugant Fitness, and Community Assembly of Genetically Bioaugmented Soil Microbes for PAH Bioremediation Open
Many polycyclic aromatic hydrocarbons (PAHs) in the environment resulting from crude oil spills and the incomplete combustion of organic matter are highly toxic, mutagenic, or carcinogenic to microorganisms and humans. Bioremediation of PA…
View article: Wastewater surveillance for public health: <i>Quo Vadis</i>?
Wastewater surveillance for public health: <i>Quo Vadis</i>? Open
Editor-in-Chief Graham Gagnon introduces this Environmental Science: Water Research & Technology themed issue on wastewater surveillance.
View article: Uncovering dynamics between SARS-CoV-2 wastewater concentrations and community infections via Bayesian spatial functional concurrent regression
Uncovering dynamics between SARS-CoV-2 wastewater concentrations and community infections via Bayesian spatial functional concurrent regression Open
Monitoring wastewater concentrations of SARS-CoV-2 yields a low-cost, noninvasive method for tracking disease prevalence and provides early warning signs of upcoming outbreaks in the serviced communities. There is tremendous clinical and p…
View article: Assessment and application of GeneXpert rapid testing for respiratory viruses in school wastewater
Assessment and application of GeneXpert rapid testing for respiratory viruses in school wastewater Open
The GeneXpert system is cost-effective for low numbers of samples and can be used for wastewater monitoring of respiratory viruses in schools. The results were reproducible and comparably sensitive to filtration-ddPCR for SARS-CoV-2 and in…
View article: Implementing an Alert System for Communicating Actionable Wastewater Surveillance Results to School Communities, Houston, Texas, 2023-2024
Implementing an Alert System for Communicating Actionable Wastewater Surveillance Results to School Communities, Houston, Texas, 2023-2024 Open
In 2020, the Houston Health Department (HHD) in Texas launched a citywide wastewater surveillance program, including a pilot program that monitored manholes at schools in Houston’s largest school district (prekindergarten–12th grade). By 2…
View article: Comparative analysis of culture- and ddPCR-based wastewater surveillance for carbapenem-resistant bacteria
Comparative analysis of culture- and ddPCR-based wastewater surveillance for carbapenem-resistant bacteria Open
This study compares culture-based and ddPCR methods for quantifying carbapenem resistance in wastewater, revealing weak correlations. Nanopore sequencing clarifies resistance mechanisms, emphasizing method selection and integrated AR surve…
View article: Wastewater Target Pathogens of Public Health Importance for Expanded Sampling, Houston, Texas, USA
Wastewater Target Pathogens of Public Health Importance for Expanded Sampling, Houston, Texas, USA Open
Building on the success of initiatives put forth during the COVID-19 pandemic response, US health officials are expanding wastewater surveillance programs to track other target pathogens and diseases of public health interest. The Houston …
View article: Olivar: towards automated variant aware primer design for multiplex tiled amplicon sequencing of pathogens
Olivar: towards automated variant aware primer design for multiplex tiled amplicon sequencing of pathogens Open
Tiled amplicon sequencing has served as an essential tool for tracking the spread and evolution of pathogens. Over 15 million complete SARS-CoV-2 genomes are now publicly available, most sequenced and assembled via tiled amplicon sequencin…
View article: Comparative analysis of culture- and ddPCR-based wastewater surveillance for carbapenem-resistant bacteria
Comparative analysis of culture- and ddPCR-based wastewater surveillance for carbapenem-resistant bacteria Open
With the widespread use of last-resort antibiotics, carbapenems, clinical reports of infections associated with carbapenem-resistant Enterobacterales (CRE) have increased. Clinical surveillance for CRE involves susceptibility testing and/o…
View article: A Novel Framework for Internal Responses to Detection of Pathogens in Wastewater by Public Health Agencies
A Novel Framework for Internal Responses to Detection of Pathogens in Wastewater by Public Health Agencies Open
Objectives: To build on the success of wastewater surveillance during the COVID-19 pandemic, jurisdictions funded under the Centers for Disease Control and Prevention National Wastewater Surveillance System are looking to expand their wast…
View article: Multiplexed detection, partitioning, and persistence of wild type and vaccine strains of measles, mumps, and rubella viruses in wastewater
Multiplexed detection, partitioning, and persistence of wild type and vaccine strains of measles, mumps, and rubella viruses in wastewater Open
Wastewater surveillance of vaccine-preventable diseases may provide early warning of outbreaks and identify areas to target for immunization. To advance wastewater monitoring of measles, mumps, and rubella viruses, we developed and validat…
View article: Long-duration environmental biosensing by recording analyte detection in DNA using recombinase memory
Long-duration environmental biosensing by recording analyte detection in DNA using recombinase memory Open
Microbial biosensors that convert environmental information into real-time visual outputs are limited in their sensing abilities in complex environments, such as soil and wastewater, due to optical inaccessibility. Biosensors that could re…
View article: Wastewater surveillance for bacterial targets: current challenges and future goals
Wastewater surveillance for bacterial targets: current challenges and future goals Open
Wastewater-based epidemiology (WBE) expanded rapidly in response to the COVID-19 pandemic. As the public health emergency has ended, researchers and practitioners are looking to shift the focus of existing wastewater surveillance programs …
View article: Wastewater surveillance for bacterial targets: current challenges and future goals
Wastewater surveillance for bacterial targets: current challenges and future goals Open
Wastewater-based epidemiology (WBE) expanded rapidly in response to the COVID-19 pandemic. As the public health emergency has ended, researchers and practitioners are looking to shift the focus of existing wastewater surveillance programs …
View article: Online trend estimation and detection of trend deviations in sub-sewershed time series of SARS-CoV-2 RNA measured in wastewater
Online trend estimation and detection of trend deviations in sub-sewershed time series of SARS-CoV-2 RNA measured in wastewater Open
Wastewater surveillance has proven a key public health tool to understand a wide range of community health diseases and has proven to be especially critical to health departments throughout the SARS CoV-2 pandemic. The size of the populati…
View article: Sensitivity and consistency of long- and short-read metagenomics and epicPCR for the detection of antibiotic resistance genes and their bacterial hosts in wastewater
Sensitivity and consistency of long- and short-read metagenomics and epicPCR for the detection of antibiotic resistance genes and their bacterial hosts in wastewater Open
Wastewater surveillance is a powerful tool to assess the risks associated with antibiotic resistance in communities. One challenge is selecting which analytical tool to deploy to measure risk indicators, such as antibiotic resistance genes…
View article: Long duration environmental biosensing by recording analyte detection in DNA using recombinase memory
Long duration environmental biosensing by recording analyte detection in DNA using recombinase memory Open
Microbial biosensors that convert environmental information into real-time visual outputs are limited in their sensing abilities in complex environments, such as soil and wastewater. Alternative reporter outputs are needed that stably reco…