Laurie K. Read
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View article: DRBD18 acts as a transcript-specific RNA editing auxiliary factor in<i>Trypanosoma brucei</i>
DRBD18 acts as a transcript-specific RNA editing auxiliary factor in<i>Trypanosoma brucei</i> Open
Uridine insertion/deletion (U-indel) RNA editing of mitochondrial transcripts is a posttranscriptional modification in kinetoplastid organisms, resulting in the generation of mature mRNAs from cryptic precursors. This RNA editing process i…
View article: KRBP72 facilitates ATPase-dependent editing progression through a structural roadblock in mitochondrial A6 mRNA
KRBP72 facilitates ATPase-dependent editing progression through a structural roadblock in mitochondrial A6 mRNA Open
Uridine insertion/deletion editing of mitochondrial messenger RNAs (mRNAs) in kinetoplastids entails the coordinated action of three complexes. RNA Editing Catalytic Complexes (RECCs) catalyze the enzymatic reactions, while the RNA Editing…
View article: Life stage–specific poly(A) site selection regulated by<i>Trypanosoma brucei</i>DRBD18
Life stage–specific poly(A) site selection regulated by<i>Trypanosoma brucei</i>DRBD18 Open
The kinetoplastid parasite, Trypanosoma brucei , undergoes a complex life cycle entailing slender and stumpy bloodstream forms in mammals and procyclic and metacyclic forms (MFs) in tsetse fly hosts. The numerous gene regulatory events tha…
View article: RESC14 and RESC8 cooperate to mediate RESC function and dynamics during trypanosome RNA editing
RESC14 and RESC8 cooperate to mediate RESC function and dynamics during trypanosome RNA editing Open
Mitochondrial transcripts in Trypanosoma brucei require extensive uridine insertion/deletion RNA editing to generate translatable open reading frames. The RNA editing substrate binding complex (RESC) serves as the scaffold that coordinates…
View article: A role for a Trypanosoma brucei cytosine RNA methyltransferase homolog in ribosomal RNA processing
A role for a Trypanosoma brucei cytosine RNA methyltransferase homolog in ribosomal RNA processing Open
In Trypanosoma brucei , gene expression is primarily regulated posttranscriptionally making RNA metabolism critical. T . brucei has an epitranscriptome containing modified RNA bases. Yet, the identity of the enzymes catalyzing modified RNA…
View article: Developmental dynamics of mitochondrial mRNA abundance and editing reveal roles for temperature and the differentiation-repressive kinase RDK1 in cytochrome oxidase subunit II mRNA editing
Developmental dynamics of mitochondrial mRNA abundance and editing reveal roles for temperature and the differentiation-repressive kinase RDK1 in cytochrome oxidase subunit II mRNA editing Open
Developmental regulation of mitochondrial uridine insertion/deletion editing in Trypanosoma brucei is necessary to modulate parasite metabolism as it shifts from dependence on glycolysis for ATP production in the mammalian bloodstream form…
View article: Translational control by <i>Trypanosoma brucei</i> DRBD18 contributes to the maintenance of the procyclic state
Translational control by <i>Trypanosoma brucei</i> DRBD18 contributes to the maintenance of the procyclic state Open
Trypanosoma brucei occupies distinct niches throughout its life cycle, within both the mammalian and tsetse fly hosts. The immunological and biochemical complexity and variability of each of these environments require a reshaping of the pr…
View article: KREH1 RNA helicase activity promotes utilization of initiator gRNAs across multiple mRNAs in trypanosome RNA editing
KREH1 RNA helicase activity promotes utilization of initiator gRNAs across multiple mRNAs in trypanosome RNA editing Open
Mitochondrial U-indel RNA editing in kinetoplastid protozoa is directed by trans-acting gRNAs and mediated by a holoenzyme with associated factors. Here, we examine the function of the holoenzyme-associated KREH1 RNA helicase in U-indel ed…
View article: Translational control by<i>Trypanosoma brucei</i>DRBD18 contributes to the maintenance of the procyclic state
Translational control by<i>Trypanosoma brucei</i>DRBD18 contributes to the maintenance of the procyclic state Open
Trypanosoma brucei occupies distinct niches throughout its life cycle, within both the mammalian and tsetse fly hosts. The immunological and biochemical complexity and variability of each of these environments require a reshaping of the pr…
View article: Conserved and transcript-specific functions of the RESC factors, RESC13 and RESC14, in kinetoplastid RNA editing
Conserved and transcript-specific functions of the RESC factors, RESC13 and RESC14, in kinetoplastid RNA editing Open
Uridine insertion/deletion RNA editing is an extensive post-transcriptional modification of mitochondrial mRNAs in kinetoplastid organisms, including Trypanosoma brucei . This process is carried out using trans -acting gRNAs and complex pr…
View article: Conserved and transcript-specific functions of the RESC factors, RESC13 and RESC14, in kinetoplastid RNA editing
Conserved and transcript-specific functions of the RESC factors, RESC13 and RESC14, in kinetoplastid RNA editing Open
Uridine insertion/deletion RNA editing is an extensive post-transcriptional modification of mitochondrial mRNAs in kinetoplastid organisms, including Trypanosoma brucei . This process is carried out using trans -acting gRNAs and complex pr…
View article: Selective nuclear export of mRNAs is promoted by DRBD18 in <i>Trypanosoma brucei</i>
Selective nuclear export of mRNAs is promoted by DRBD18 in <i>Trypanosoma brucei</i> Open
Kinetoplastids, including Trypanosoma brucei , control gene expression primarily at the posttranscriptional level. Nuclear mRNA export is an important, but understudied, step in this process. The general heterodimeric export factors, Mex67…
View article: Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei
Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei Open
Kinetoplastids, including Trypanosoma brucei, control gene expression primarily at the posttranscriptional level. Nuclear mRNA export is an important, but understudied, step in this process. The general heterodimeric export factors, Mex67/…
View article: Trypanosome RNAEditing Substrate Binding Complex integrity and function depends on the upstream action of RESC10
Trypanosome RNAEditing Substrate Binding Complex integrity and function depends on the upstream action of RESC10 Open
Uridine insertion/deletion editing of mitochondrial mRNAs is a characteristic feature of kinetoplastids, including Trypanosoma brucei. Editing is directed by trans-acting gRNAs and catalyzed by related RNA Editing Core Complexes (RECCs). T…
View article: Developmental regulation of edited CYb and COIII mitochondrial mRNAs is achieved by distinct mechanisms in Trypanosoma brucei
Developmental regulation of edited CYb and COIII mitochondrial mRNAs is achieved by distinct mechanisms in Trypanosoma brucei Open
Trypanosoma brucei is a parasitic protozoan that undergoes a complex life cycle involving insect and mammalian hosts that present dramatically different nutritional environments. Mitochondrial metabolism and gene expression are highly regu…
View article: Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes
Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes Open
View article: Structural Basis of Protein Arginine Methyltransferase Activation by a Catalytically Dead Homolog (Prozyme)
Structural Basis of Protein Arginine Methyltransferase Activation by a Catalytically Dead Homolog (Prozyme) Open
View article: Structural Basis of Protein Arginine Methyltransferase Activation by a Catalytically Dead Homolog (Prozyme)
Structural Basis of Protein Arginine Methyltransferase Activation by a Catalytically Dead Homolog (Prozyme) Open
Prozymes are pseudoenzymes that stimulate the function of weakly active enzymes through complex formation. The major Trypanosoma brucei protein arginine methyltransferase, Tb PRMT1 enzyme (ENZ), requires Tb PRMT1 prozyme (PRO) to form an a…
View article: MRB10130 is a RESC assembly factor that promotes kinetoplastid RNA editing initiation and progression
MRB10130 is a RESC assembly factor that promotes kinetoplastid RNA editing initiation and progression Open
Uridine insertion deletion editing in kinetoplastid protozoa requires a complex machinery, a primary component of which is the RNA editing substrate binding complex (RESC). RESC contains two modules termed GRBC (guide RNA binding complex) …
View article: Intrinsic and regulated properties of minimally edited trypanosome mRNAs
Intrinsic and regulated properties of minimally edited trypanosome mRNAs Open
Most mitochondrial mRNAs in kinetoplastids require extensive uridine insertion/deletion editing to generate translatable open reading frames. Editing is specified by trans-acting gRNAs and involves a complex machinery including basal and a…
View article: Trypanosoma brucei PRMT1 Is a Nucleic Acid Binding Protein with a Role in Energy Metabolism and the Starvation Stress Response
Trypanosoma brucei PRMT1 Is a Nucleic Acid Binding Protein with a Role in Energy Metabolism and the Starvation Stress Response Open
Trypanosoma brucei infection causes human African trypanosomiasis, also known as sleeping sickness, a disease with a nearly 100% fatality rate when untreated. Current drugs are expensive, toxic, and highly impractical to administer, prompt…
View article: High throughput sequencing revolution reveals conserved fundamentals of U‐indel editing
High throughput sequencing revolution reveals conserved fundamentals of U‐indel editing Open
Among Euglenozoans, mitochondrial RNA editing occurs in the diplonemids and in the kinetoplastids that include parasitic trypanosomes. Yet U‐indel editing, in which open reading frames (ORFs) on mRNAs are generated by insertion and deletio…
View article: MRB7260 is essential for productive protein–RNA interactions within the RNA editing substrate binding complex during trypanosome RNA editing
MRB7260 is essential for productive protein–RNA interactions within the RNA editing substrate binding complex during trypanosome RNA editing Open
The trypanosome R NA e diting s ubstrate binding c omplex (RESC) acts as the platform for mitochondrial uridine insertion/deletion RNA editing and facilitates the protein–protein and protein–RNA interactions required for the editing proces…
View article: Trypanosome RNA Editing Mediator Complex proteins have distinct functions in gRNA utilization
Trypanosome RNA Editing Mediator Complex proteins have distinct functions in gRNA utilization Open
Uridine insertion/deletion RNA editing is an essential process in kinetoplastid parasites whereby mitochondrial mRNAs are modified through the specific insertion and deletion of uridines to generate functional open reading frames, many of …
View article: The Major Protein Arginine Methyltransferase in Trypanosoma brucei Functions as an Enzyme-Prozyme Complex
The Major Protein Arginine Methyltransferase in Trypanosoma brucei Functions as an Enzyme-Prozyme Complex Open
Prozymes are catalytically inactive enzyme paralogs that dramatically stimulate the function of weakly active enzymes through complex formation. The two prozymes described to date reside in the polyamine biosynthesis pathway of the human p…
View article: High-throughput sequencing of partially edited trypanosome mRNAs reveals barriers to editing progression and evidence for alternative editing
High-throughput sequencing of partially edited trypanosome mRNAs reveals barriers to editing progression and evidence for alternative editing Open
Uridine insertion/deletion RNA editing in kinetoplastids entails the addition and deletion of uridine residues throughout the length of mitochondrial transcripts to generate translatable mRNAs. This complex process requires the coordinated…
View article: REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes
REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes Open
Mitochondrial mRNAs in Trypanosoma brucei undergo extensive insertion and deletion of uridylates that are catalyzed by the RNA editing core complex (RECC) and directed by hundreds of small guide RNAs (gRNAs) that base pair with mRNA. RECC …
View article: Trypanosome <scp>RNA</scp> editing: the complexity of getting U in and taking U out
Trypanosome <span>RNA</span> editing: the complexity of getting U in and taking U out Open
RNA editing, which adds sequence information to RNAs post‐transcriptionally, is a widespread phenomenon throughout eukaryotes. The most complex form of this process is the uridine (U) insertion/deletion editing that occurs in the mitochond…
View article: Integrity of the core mitochondrial RNA-binding complex 1 is vital for trypanosome RNA editing
Integrity of the core mitochondrial RNA-binding complex 1 is vital for trypanosome RNA editing Open
Trypanosoma brucei is the causative agent of the human and veterinarian diseases African sleeping sickness and nagana. A majority of its mitochondrial-encoded transcripts undergo RNA editing, an essential process of post-transcriptional ur…
View article: Structures of the<i>T. brucei</i>kRNA editing factor MRB1590 reveal unique RNA-binding pore motif contained within an ABC-ATPase fold
Structures of the<i>T. brucei</i>kRNA editing factor MRB1590 reveal unique RNA-binding pore motif contained within an ABC-ATPase fold Open
Kinetoplastid RNA (kRNA) editing is a process that creates translatable mitochondrial mRNA transcripts from cryptogene encoded RNAs and is unique for kinetoplastids, such as Trypanosoma brucei. In addition to the catalytic 20S editosome, m…