Lawrence J. Stern
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View article: TCR repertoire of human cytotoxic CD4 T cells responding to betaherpesviruses HHV-6B and HCMV
TCR repertoire of human cytotoxic CD4 T cells responding to betaherpesviruses HHV-6B and HCMV Open
Background The role of CD4 T cells in the control of viral infections beyond their traditional helper activity has been increasingly recognized, and CD4 T cells with cytotoxic capacity have been reported for the nearly ubiquitous betaherpe…
View article: The Profiling of MHC-I and MHC-II peptide repertoire of Cytokine-Stressed Human Pancreatic Islets 3593
The Profiling of MHC-I and MHC-II peptide repertoire of Cytokine-Stressed Human Pancreatic Islets 3593 Open
Description Type 1 diabetes (T1D) arises from T cell-mediated destruction of insulin-secreting pancreatic beta cells. Inflammation is hypothesized to induced presentation of new canonical or non-canonical epitopes or an imbalance of certai…
View article: Develop and validate DNA-barcode labelled MHCII multimers for detection of antigen-specific CD4 T cells displayed towards large libraries of epitopes 4185
Develop and validate DNA-barcode labelled MHCII multimers for detection of antigen-specific CD4 T cells displayed towards large libraries of epitopes 4185 Open
Description Here we demonstrate the method for broad-scale detection of antigen-specific CD4 T cells. We have established a protocol for MHCII production and peptide exchange (pMHCII complexes), which are multimerized on DNA barcode- and f…
View article: Conformational variants of I-Ak MHC class II molecules carry distinct immunopeptidomes
Conformational variants of I-Ak MHC class II molecules carry distinct immunopeptidomes Open
Major histocompatibility (MHC) class II molecules can exist in two distinct conformational states based on alternative pairing of transmembrane domain GxxxG dimerization motifs (i.e., M1- and M2-paired MHC class II). M1- and M2-paired MHC …
View article: Exploring the role of T cells in Alzheimer's and other neurodegenerative diseases: Emerging therapeutic insights from the T Cells in the Brain symposium
Exploring the role of T cells in Alzheimer's and other neurodegenerative diseases: Emerging therapeutic insights from the T Cells in the Brain symposium Open
This proceedings article summarizes the inaugural “T Cells in the Brain” symposium held at Columbia University. Experts gathered to explore the role of T cells in neurodegenerative diseases. Key topics included characterization of antigen‐…
View article: The HHV-6B U20 glycoprotein binds ULBP1, masking it from recognition by NKG2D and interfering with natural killer cell activation
The HHV-6B U20 glycoprotein binds ULBP1, masking it from recognition by NKG2D and interfering with natural killer cell activation Open
Introduction Human Herpesvirus 6B (HHV-6B) impedes host immune responses by downregulating class I MHC molecules (MHC-I), hindering antigen presentation to CD8+ T cells. Downregulation of MHC-I disengages inhibitory receptors on natural ki…
View article: Immunopeptidome profiling of human coronavirus OC43-infected cells identifies CD4 T-cell epitopes specific to seasonal coronaviruses or cross-reactive with SARS-CoV-2
Immunopeptidome profiling of human coronavirus OC43-infected cells identifies CD4 T-cell epitopes specific to seasonal coronaviruses or cross-reactive with SARS-CoV-2 Open
Seasonal “common-cold” human coronaviruses are widely spread throughout the world and are mainly associated with mild upper respiratory tract infections. The emergence of highly pathogenic coronaviruses MERS-CoV, SARS-CoV, and most recentl…
View article: Inhibited MHC Class I and MHC Class II antigen processing and presentation upon Sars-CoV-2 infection
Inhibited MHC Class I and MHC Class II antigen processing and presentation upon Sars-CoV-2 infection Open
Major histocompatibility (MHC) bound viral peptides are the immune signatures to initiate the selective and cross-reactive T cell responses. Identifying the antigenic targets of adaptive immunity to SARS-CoV-2 is a key towards understandin…
View article: CD4 Effector TCR Avidity for Peptide on APC Determines the Level of Memory Generated
CD4 Effector TCR Avidity for Peptide on APC Determines the Level of Memory Generated Open
Initial TCR affinity for peptide Ag is known to impact the generation of memory; however, its contributions later, when effectors must again recognize Ag at 5–8 d postinfection to become memory, is unclear. We examined whether the effector…
View article: Immunopeptidome profiling of human coronavirus OC43-infected cells identifies CD4 T cell epitopes specific to seasonal coronaviruses or cross-reactive with SARS-CoV-2
Immunopeptidome profiling of human coronavirus OC43-infected cells identifies CD4 T cell epitopes specific to seasonal coronaviruses or cross-reactive with SARS-CoV-2 Open
Seasonal “common-cold” human coronaviruses are widely spread throughout the world and are mainly associated with mild upper respiratory tract infections. The emergence of highly pathogenic coronaviruses MERS-CoV, SARS-CoV, and most recentl…
View article: PDIA3 epitope-driven immune autoreactivity contributes to hepatic damage in type 2 diabetes
PDIA3 epitope-driven immune autoreactivity contributes to hepatic damage in type 2 diabetes Open
A diet rich in saturated fat and carbohydrates causes low-grade chronic inflammation in several organs, including the liver, ultimately driving nonalcoholic steatohepatitis. In this setting, environment-driven lipotoxicity and glucotoxicit…
View article: Phenylsulfamoyl Benzoic Acid Inhibitor of ERAP2 with a Novel Mode of Inhibition
Phenylsulfamoyl Benzoic Acid Inhibitor of ERAP2 with a Novel Mode of Inhibition Open
ERAP1 and ERAP2 are endoplasmic reticulum zinc-binding aminopeptidases that play crucial roles in processing peptides for loading onto class I major histocompatibility complex proteins. These enzymes are therapeutic targets in cancer and a…
View article: Corrigendum: Structural Models for Roseolovirus U20 And U21: Non-Classical MHC-I Like Proteins From HHV-6A, HHV-6B, and HHV-7
Corrigendum: Structural Models for Roseolovirus U20 And U21: Non-Classical MHC-I Like Proteins From HHV-6A, HHV-6B, and HHV-7 Open
[This corrects the article DOI: 10.3389/fimmu.2022.864898.].
View article: Peptide Avidity for TCR on CD4 Effectors Determines the Extent of Memory Generation
Peptide Avidity for TCR on CD4 Effectors Determines the Extent of Memory Generation Open
The impact of initial peptide antigen affinity for TCR in driving memory fate has been studied previously, however its contributions when effectors contract to memory is unclear. To become memory, effector CD4 T cells must recognize antige…
View article: Structural Models for Roseolovirus U20 And U21: Non-Classical MHC-I Like Proteins From HHV-6A, HHV-6B, and HHV-7
Structural Models for Roseolovirus U20 And U21: Non-Classical MHC-I Like Proteins From HHV-6A, HHV-6B, and HHV-7 Open
Human roseolovirus U20 and U21 are type I membrane glycoproteins that have been implicated in immune evasion by interfering with recognition of classical and non-classical MHC proteins. U20 and U21 are predicted to be type I glycoproteins …
View article: Broadly-recognized, cross-reactive SARS-CoV-2 CD4 T cell epitopes are highly conserved across human coronaviruses and presented by common HLA alleles
Broadly-recognized, cross-reactive SARS-CoV-2 CD4 T cell epitopes are highly conserved across human coronaviruses and presented by common HLA alleles Open
Summary Sequence homology between SARS-CoV-2 and common-cold human coronaviruses (HCoVs) raises the possibility that memory responses to prior HCoV infection can impact the T cell response in COVID-19. We studied T cells recognizing SARS-C…
View article: Distinguishing Signal From Noise in Immunopeptidome Studies of Limiting-Abundance Biological Samples: Peptides Presented by I-Ab in C57BL/6 Mouse Thymus
Distinguishing Signal From Noise in Immunopeptidome Studies of Limiting-Abundance Biological Samples: Peptides Presented by I-Ab in C57BL/6 Mouse Thymus Open
Antigen presentation by MHC-II proteins in the thymus is central to selection of CD4 T cells, but analysis of the full repertoire of presented peptides responsible for positive and negative selection is complicated by the low abundance of …
View article: Human antibody immune responses are personalized by selective removal of MHC-II peptide epitopes
Human antibody immune responses are personalized by selective removal of MHC-II peptide epitopes Open
Summary Human antibody responses are established by the generation of combinatorial sequence diversity in antibody variable domains, followed by iterative rounds of mutation and selection via T cell recognition of antigen peptides presente…
View article: Single-particle cryo-EM studies of ERp44-ERAP1 and ERp44-ERAP2 reveal their ER-retention mechanism and structural dynamics involved
Single-particle cryo-EM studies of ERp44-ERAP1 and ERp44-ERAP2 reveal their ER-retention mechanism and structural dynamics involved Open
ERAP1 is an ER-resident aminopeptidase with roles in antigenic peptide processing for MHC-I presentation and angiotensin processing for blood pressure control.Previous studies had implicated ERp44 C29 in the thioredoxinlike a-domain and ER…
View article: Structural Basis of Inhibition of Insulin-Regulated Aminopeptidase by a Macrocyclic Peptidic Inhibitor
Structural Basis of Inhibition of Insulin-Regulated Aminopeptidase by a Macrocyclic Peptidic Inhibitor Open
Insulin-regulated aminopeptidase (IRAP) is a transmembrane zinc metallopeptidase with many important biological functions and an emerging pharmacological target. Although previous structural studies have given insight on how IRAP recognize…