Lázaro Betancourt
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View article: Distant metastases of melanoma exhibit varying extent of intrapatient proteogenomic heterogeneity
Distant metastases of melanoma exhibit varying extent of intrapatient proteogenomic heterogeneity Open
Background Metastatic melanoma is a highly aggressive disease with poor survival rates despite recent therapeutic advancements with immunotherapy. The proteomic landscape of advanced melanoma remains poorly understood, especially regarding…
View article: Mitochondrial proteome landscape unveils key insights into melanoma severity and treatment strategies
Mitochondrial proteome landscape unveils key insights into melanoma severity and treatment strategies Open
Background Melanoma, the deadliest form of skin cancer, exhibits resistance to conventional therapies, particularly in advanced and metastatic stages. Mitochondrial pathways, including oxidative phosphorylation and mitochondrial translatio…
View article: The melanoma MEGA-study: Integrating proteogenomics, digital pathology, and AI-analytics for precision oncology
The melanoma MEGA-study: Integrating proteogenomics, digital pathology, and AI-analytics for precision oncology Open
View article: Melanoma Proteomics Unveiled: Harmonizing Diverse Data Sets for Biomarker Discovery and Clinical Insights via MEL-PLOT
Melanoma Proteomics Unveiled: Harmonizing Diverse Data Sets for Biomarker Discovery and Clinical Insights via MEL-PLOT Open
Using several melanoma proteomics data sets we created a single analysis platform that enables the discovery, knowledge build, and validation of diagnostic, predictive, and prognostic biomarkers at the protein level. Quantitative mass-spec…
View article: Proteogenomic Profiling of Treatment-Naïve Metastatic Malignant Melanoma
Proteogenomic Profiling of Treatment-Naïve Metastatic Malignant Melanoma Open
Background: Melanoma is a highly heterogeneous disease, and a deeper molecular classification is essential for improving patient stratification and treatment approaches. Here, we describe the histopathology-driven proteogenomic landscape o…
View article: Unbiased Drug Target Prediction Reveals Sensitivity to Ferroptosis Inducers, HDAC and RTK Inhibitors in Melanoma Subtypes
Unbiased Drug Target Prediction Reveals Sensitivity to Ferroptosis Inducers, HDAC and RTK Inhibitors in Melanoma Subtypes Open
Background The utilization of PD1 and CTLA4 inhibitors has revolutionized the treatment of malignant melanoma (MM). However, resistance to targeted and immune‐checkpoint‐based therapies still poses a significant problem. Objective Here, we…
View article: In Vivo Photopolymerization: Achieving Detailed Conducting Patterns for Bioelectronics
In Vivo Photopolymerization: Achieving Detailed Conducting Patterns for Bioelectronics Open
Bioelectronics holds great potential as therapeutics, but introducing conductive structures within the body poses great challenges. While implanted rigid and substrate‐bound electrodes often result in inflammation and scarring in vivo, the…
View article: Predicting immune checkpoint therapy response in three independent metastatic melanoma cohorts
Predicting immune checkpoint therapy response in three independent metastatic melanoma cohorts Open
Introduction While Immune checkpoint inhibition (ICI) therapy shows significant efficacy in metastatic melanoma, only about 50% respond, lacking reliable predictive methods. We introduce a panel of six proteins aimed at predicting response…
View article: Mitochondrial dysfunction and immune suppression in BRAF V600E‐mutated metastatic melanoma
Mitochondrial dysfunction and immune suppression in BRAF V600E‐mutated metastatic melanoma Open
Our investigation into the mitochondrial proteome dynamics in metastatic melanoma reveals profound metabolic reprogramming that is central to the progression and treatment resistance of BRAF V600E-mutated tumours. These findings highlight …
View article: Predicting immune checkpoint therapy response in three independent metastatic melanoma cohorts
Predicting immune checkpoint therapy response in three independent metastatic melanoma cohorts Open
While Immune checkpoint inhibition (ICI) therapy shows significant efficacy in metastatic melanoma, only about 50% respond, lacking reliable predictive methods. We introduce a panel of six proteins aimed at predicting response to ICI thera…
View article: Identifying Ferroptosis Inducers, HDAC, and RTK Inhibitor Sensitivity in Melanoma Subtypes through Unbiased Drug Target Prediction
Identifying Ferroptosis Inducers, HDAC, and RTK Inhibitor Sensitivity in Melanoma Subtypes through Unbiased Drug Target Prediction Open
The utilization of PD1 and CTLA4 inhibitors has revolutionized the treatment of malignant melanoma (MM). However, resistance to targeted and immune-checkpoint-based therapies still poses a significant problem. Here we mine large scale MM p…
View article: Histopathology-assisted proteogenomics provides foundations for stratification of melanoma metastases
Histopathology-assisted proteogenomics provides foundations for stratification of melanoma metastases Open
Here we describe the histopathology-driven proteogenomic landscape of 142 treatment-naïve metastatic melanoma samples. We identified five proteomic subtypes that integrate the immune and stroma microenvironment components, and associate wi…
View article: A time-efficient workflow to purify a library of alanine mutants of surface-exposed residues of the domain III of the envelope protein of dengue virus serotype 1
A time-efficient workflow to purify a library of alanine mutants of surface-exposed residues of the domain III of the envelope protein of dengue virus serotype 1 Open
Dengue complex is formed by four viral serotypes that cause the disease of the same name. Dengue is the arthropod-borne disease with the highest incidence worldwide. The envelope glycoprotein comprises three structural domains. The domain …
View article: Proteogenomic Characterization Reveals Therapeutic Opportunities Related to Mitochondrial Function in Melanoma
Proteogenomic Characterization Reveals Therapeutic Opportunities Related to Mitochondrial Function in Melanoma Open
Summary The dynamics of more than 1900 mitochondrial proteins was explored through quantitative proteomics in 151 melanoma-related tissue samples of both surgical and autopsy origin. Dysregulation of mitochondrial pathways in primary tumor…
View article: Method Matters: Exploring Alkoxysulfonate-Functionalized Poly(3,4-ethylenedioxythiophene) and Its Unintentional Self-Aggregating Copolymer toward Injectable Bioelectronics
Method Matters: Exploring Alkoxysulfonate-Functionalized Poly(3,4-ethylenedioxythiophene) and Its Unintentional Self-Aggregating Copolymer toward Injectable Bioelectronics Open
Injectable bioelectronics could become an alternative or a complement to traditional drug treatments. To this end, a new self-doped p-type conducting PEDOT-S copolymer (A5) was synthesized. This copolymer formed highly water-dispers…
View article: Mapping the Melanoma Plasma Proteome (MPP) Using Single-Shot Proteomics Interfaced with the WiMT Database
Mapping the Melanoma Plasma Proteome (MPP) Using Single-Shot Proteomics Interfaced with the WiMT Database Open
Plasma analysis by mass spectrometry-based proteomics remains a challenge due to its large dynamic range of 10 orders in magnitude. We created a methodology for protein identification known as Wise MS Transfer (WiMT). Melanoma plasma sampl…
View article: Deep Proteomic Analysis on Biobanked Paraffine-Archived Melanoma with Prognostic/Predictive Biomarker Read-Out
Deep Proteomic Analysis on Biobanked Paraffine-Archived Melanoma with Prognostic/Predictive Biomarker Read-Out Open
The discovery of novel protein biomarkers in melanoma is crucial. Our introduction of formalin-fixed paraffin-embedded (FFPE) tumor protocol provides new opportunities to understand the progression of melanoma and open the possibility to s…
View article: A biobanking turning‐point in the use of formalin‐fixed, paraffin tumor blocks to unveil kinase signaling in melanoma
A biobanking turning‐point in the use of formalin‐fixed, paraffin tumor blocks to unveil kinase signaling in melanoma Open
Malignant melanoma (MM) is one of the most aggressive human solid tumors, and it is associated with the highest mortality of all skin cancers.1 Aberrant patterns of protein expression and posttranslational modifications (PTMs) have been li…
View article: The human melanoma proteome atlas—Defining the molecular pathology
The human melanoma proteome atlas—Defining the molecular pathology Open
The MM500 study is an initiative to map the protein levels in malignant melanoma tumor samples, focused on in‐depth histopathology coupled to proteome characterization. The protein levels and localization were determined for a broad spectr…
View article: The Human Melanoma Proteome Atlas—Complementing the melanoma transcriptome
The Human Melanoma Proteome Atlas—Complementing the melanoma transcriptome Open
The MM500 meta‐study aims to establish a knowledge basis of the tumor proteome to serve as a complement to genome and transcriptome studies. Somatic mutations and their effect on the transcriptome have been extensively characterized in mel…
View article: Proteogenomics Reveals how Metastatic Melanoma Modulates the Immune System to Allow Immune Evasion
Proteogenomics Reveals how Metastatic Melanoma Modulates the Immune System to Allow Immune Evasion Open
Summary Malignant melanoma (MM) develops from the melanocytes and in its advanced stage is the most aggressive type of skin cancer. Here we report a comprehensive analysis on a prospective cohort study, including non-tumor, primary and met…
View article: Protein Expression in Metastatic Melanoma and the Link to Disease Presentation in a Range of Tumor Phenotypes
Protein Expression in Metastatic Melanoma and the Link to Disease Presentation in a Range of Tumor Phenotypes Open
Malignant melanoma is among the most aggressive skin cancers and it has among the highest metastatic potentials. Although surgery to remove the primary tumor is the gold standard treatment, once melanoma progresses and metastasizes to the …
View article: The Hidden Story of Heterogeneous B-raf V600E Mutation Quantitative Protein Expression in Metastatic Melanoma—Association with Clinical Outcome and Tumor Phenotypes
The Hidden Story of Heterogeneous B-raf V600E Mutation Quantitative Protein Expression in Metastatic Melanoma—Association with Clinical Outcome and Tumor Phenotypes Open
In comparison to other human cancer types, malignant melanoma exhibits the greatest amount of heterogeneity. After DNA-based detection of the BRAF V600E mutation in melanoma patients, targeted inhibitor treatment is the current recommendat…
View article: The Hidden Story on Heterogeneous B-raf V600E Mutation Quantitative Protein Expression in Metastatic Melanoma – Association with Clinical Outcome and Tumor Phenotypes
The Hidden Story on Heterogeneous B-raf V600E Mutation Quantitative Protein Expression in Metastatic Melanoma – Association with Clinical Outcome and Tumor Phenotypes Open
In comparison to other human cancer types, malignant melanoma exhibits the greatest amount of heterogeneity. After DNA-based detection of the BRAF V600E mutation in melanoma patients, targeted inhibitor treatment is the current recommendat…
View article: Novel functional proteins coded by the human genome discovered in metastases of melanoma patients
Novel functional proteins coded by the human genome discovered in metastases of melanoma patients Open
View article: Improved survival prognostication of node-positive malignant melanoma patients utilizing shotgun proteomics guided by histopathological characterization and genomic data
Improved survival prognostication of node-positive malignant melanoma patients utilizing shotgun proteomics guided by histopathological characterization and genomic data Open
View article: Clinical protein science in translational medicine targeting malignant melanoma
Clinical protein science in translational medicine targeting malignant melanoma Open
View article: Correction: Automated phosphopeptide enrichment from minute quantities of frozen malignant melanoma tissue
Correction: Automated phosphopeptide enrichment from minute quantities of frozen malignant melanoma tissue Open
[This corrects the article DOI: 10.1371/journal.pone.0208562.].
View article: Automated phosphopeptide enrichment from minute quantities of frozen malignant melanoma tissue
Automated phosphopeptide enrichment from minute quantities of frozen malignant melanoma tissue Open
To acquire a deeper understanding of malignant melanoma (MM), it is essential to study the proteome of patient tissues. In particular, phosphoproteomics of MM has become of significant importance because of the central role that phosphoryl…
View article: Challenging the heterogeneity of disease presentation in malignant melanoma—impact on patient treatment
Challenging the heterogeneity of disease presentation in malignant melanoma—impact on patient treatment Open