Lea A. Rempel
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View article: A metaproteomic analysis of the piglet fecal microbiome across the weaning transition
A metaproteomic analysis of the piglet fecal microbiome across the weaning transition Open
Microbiome analysis has relied largely on metagenomics to characterize microbial populations and predict their functions. Here, we used a metaproteomic analysis of the fecal microbiome in piglets before and after weaning to compare protein…
View article: Influence of choline and follistatin supplementation during in vitro bovine oocyte maturation on oocyte competence and blastocyst development
Influence of choline and follistatin supplementation during in vitro bovine oocyte maturation on oocyte competence and blastocyst development Open
Summary Metabolite supplementation during in vitro embryo development improves blastocyst quality, however, our understanding of the incorporation of metabolites during in vitro maturation (IVM) is limited. Two important metabolites, folli…
View article: PSIII-13 Putative metabolites for sow lifetime opportunity and production traits
PSIII-13 Putative metabolites for sow lifetime opportunity and production traits Open
Identifying females with favorable lifetime born alive (opportunity) and weaned (wn; production) traits requires extended observation. Estimate of lifetime (4 parities) difference in the economic margin over genetic cost between a high pro…
View article: Weaning transition, but not the administration of probiotic candidate Kazachstania slooffiae, shaped the gastrointestinal bacterial and fungal communities in nursery piglets
Weaning transition, but not the administration of probiotic candidate Kazachstania slooffiae, shaped the gastrointestinal bacterial and fungal communities in nursery piglets Open
As in-feed antibiotics are phased out of swine production, producers are seeking alternatives to facilitate improvements in growth typically seen from this previously common feed additive. Kazachstania slooffiae is a prominent commensal fu…
View article: PSII-7 Is Scrotal Ultrasound a Useful Tool in Ram Breeding Soundness examination?
PSII-7 Is Scrotal Ultrasound a Useful Tool in Ram Breeding Soundness examination? Open
Varicocele, a condition of abnormally dilated veins within the pampiniform plexus, is associated with reduced male fertility in several species but its frequency and impact in rams is not well documented. Semen was collected and pampinifor…
View article: PSIV-18 Combination of 1.8 Mm Choline and Follistatin Supplementation May Reduce Transcript Abundance of CEPT1 and CPBE4 During In-Vitro Bovine Oocyte Maturation
PSIV-18 Combination of 1.8 Mm Choline and Follistatin Supplementation May Reduce Transcript Abundance of CEPT1 and CPBE4 During In-Vitro Bovine Oocyte Maturation Open
One-carbon metabolite supplementation during in vitro embryonic development improves blastocyst quality; however, our understanding of the effects of combining these metabolites during in vitro maturation (IVM) is limited. Supplementation …
View article: Secreted metabolome of porcine blastocysts encapsulated within in vitro 3D alginate hydrogel culture systems undergoing morphological changes provides insights into specific mechanisms involved in the initiation of porcine conceptus elongation
Secreted metabolome of porcine blastocysts encapsulated within in vitro 3D alginate hydrogel culture systems undergoing morphological changes provides insights into specific mechanisms involved in the initiation of porcine conceptus elongation Open
Context The exact mechanisms regulating the initiation of porcine conceptus elongation are not known due to the complexity of the uterine environment. Aims To identify contributing factors for initiation of conceptus elongation in vitro, t…
View article: Dam parity structure and body condition during lactation influence piglet growth and gilt sexual maturation through pre-finishing
Dam parity structure and body condition during lactation influence piglet growth and gilt sexual maturation through pre-finishing Open
Energy demands during lactation greatly influence sow body condition and piglet performance. We hypothesized that primiparous sows or sows with reduced body condition would produce piglets with reduced growth and delayed sexual maturation.…
View article: PSIX-28 Parity and lactation stage affect the sow milk microbiome
PSIX-28 Parity and lactation stage affect the sow milk microbiome Open
The milk microbiome in swine has been recently demonstrated to develop and alter the growing gut microbiome in offspring. Milk is the primary nutritional source for piglets and the established microbial communities from initial diet remain…
View article: Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
View article: Additional file 12 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 12 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 12: Table S12. Milk type DEG associated with various immune response GO terms. GO terms are given in separate tabs. DEG associated with the GO term and their log2 fold changes (milk vs colostrum) are shown.
View article: Additional file 10 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 10 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 10: Table S10. Analysis of differentially expressed genes (DEG) for the milk type main effect. DEG are highlighted in blue. Genes colored gray were filtered out for being significant for the milk type x parity interaction o…
View article: Additional file 1 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 1 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 1: Table S1. Sequencing statistics. Number of raw sequence reads, number of reads mapping to the S. scrofa 11.1 genome assembly, and percentage of mapped reads for each of the sequenced libraries.
View article: Additional file 7 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 7 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 7: Table S7. Analysis of differentially expressed transcripts (DET) for the milk type main effect. DET are highlighted in blue. Transcripts colored gray were filtered out for being significant for the milk type x parity int…
View article: Additional file 6 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 6 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 6: Table S6. Analysis of differentially expressed transcripts (DET) for the milk type x parity interaction. DET are highlighted in blue.
View article: Additional file 4 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 4 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 4: Table S4. Novel mRNA annotation. Annotation is given in GTF format.
View article: Additional file 8 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 8 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 8: Table S8. Analysis of differentially expressed transcripts (DET) for the parity main effect. DET are highlighted in blue. Transcripts colored gray were filtered out for being significant for the milk type x parity intera…
View article: Additional file 9 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 9 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 9: Table S9. Analysis of differentially expressed genes (DEG) for the milk type x parity interaction. DEG are highlighted in blue.
View article: Additional file 2 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 2 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 2: Table S2. Summary of reads mapping to RNA regions of the swine genome. Number of raw sequence reads mapping to annotated RNA regions in the S. scrofa 11.1 genome assembly. RNA classifications are based on the NCBI S. scr…
View article: Additional file 3 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 3 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 3: Table S3. Novel lncRNA annotation. Annotation is given in GTF format. LincRNA denotes intergenic lncRNA, ilncRNA denotes intronic RNA, lncNAT denotes natural antisense lncRNA, and isolncRNA denotes novel isoform lncRNA.
View article: Additional file 11 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 11 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 11: Table S11. Gene ontology (GO) term and pathway analysis for the milk type main effect. Biological process (BP), molecular function (MF), cellular component (CC), and KEGG pathway results are shown in separate tabs.
View article: Additional file 5 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities
Additional file 5 of Characterization and comparative analysis of transcriptional profiles of porcine colostrum and mature milk at different parities Open
Additional file 5: Table S5. BLAST search results for novel lncRNA against NONCODE databases. BLAST hits for novel lncRNA searched against NONCODE databases for multiple species. Entries in the species column indicate the top BLAST hit for…
View article: PSII-26 Early- and mid- lactation milk traits are associated with piglet growth during lactation
PSII-26 Early- and mid- lactation milk traits are associated with piglet growth during lactation Open
Preweaning piglet growth is tied to milk quality and consumption. To determine the relationship of milk components from parity 1–4 dams and piglet growth, early and mid-lactation (d 2 and 16) milk samples were collected from 48 litters and…
View article: 74 Epigenetic marks within the swine placenta are differentially expressed due to season
74 Epigenetic marks within the swine placenta are differentially expressed due to season Open
The objective of the current project was to validate expression of known chromatin modification genes and assess downstream targets in d45 swine placenta derived from seasonal semen collections and breedings and semen storage. Six treatmen…
View article: 75 Epigenetic marks within the swine placenta are differentially expressed due to season
75 Epigenetic marks within the swine placenta are differentially expressed due to season Open
The objective of the current project was to validate expression of known chromatin modification genes and assess downstream targets in d45 swine placenta derived from seasonal semen collections and breedings and semen storage. Six treatmen…
View article: Characterization and Comparative Analysis of Transcriptional Profiles of Porcine Colostrum and Mature Milk Across Multiple Parities
Characterization and Comparative Analysis of Transcriptional Profiles of Porcine Colostrum and Mature Milk Across Multiple Parities Open
BackgroundThe expression of genes and their regulation during lactation in swine is not well-understood. In order to gain a better understanding of genes and pathways involved in sow lactation, total RNA from colostrum and mature milk samp…
View article: Genes Associated With Chromatin Modification Within the Swine Placenta Are Differentially Expressed Due to Factors Associated With Season
Genes Associated With Chromatin Modification Within the Swine Placenta Are Differentially Expressed Due to Factors Associated With Season Open
Seasonal reproductive inefficiency is still observed in modern swine facilities. We previously reported global placental methylation activity was reduced from summer breedings and tended to be less from semen collected during cooler period…
View article: Genes associated with body weight gain and feed intake identified by meta-analysis of the mesenteric fat from crossbred beef steers
Genes associated with body weight gain and feed intake identified by meta-analysis of the mesenteric fat from crossbred beef steers Open
Mesenteric fat is a visceral fat depot that increases with cattle maturity and can be influenced by diet. There may be a relationship between the accumulation of mesenteric fat and feed efficiency in beef cattle. The purpose of this study …
View article: The effect of varicocele on semen quality in boars exposed to heat stress1
The effect of varicocele on semen quality in boars exposed to heat stress1 Open
Semen quality has a dramatic impact on reproductive efficiency in the swine industry, influencing both conception rate and litter size. The objective of this study was to assess whether the presence of varicocele hinders semen quality in b…
View article: Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation
Metabolic compounds within the porcine uterine environment are unique to the type of conceptus present during the early stages of blastocyst elongation Open
The objective of this study was to identify metabolites within the porcine uterine milieu during the early stages of blastocyst elongation. At Days 9, 10, or 11 of gestation, reproductive tracts of White cross‐bred gilts ( n = 38) were col…