Lee E. Hughes
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View article: Investigating novel <i>Streptomyces</i> bacteriophage endolysins as potential antimicrobial agents
Investigating novel <i>Streptomyces</i> bacteriophage endolysins as potential antimicrobial agents Open
As antibiotic resistance has become a major global threat, the World Health Organization (WHO) has urgently called for alternative strategies for control of bacterial infections. Endolysin, a phage-encoded protein, can degrade bacterial pe…
View article: A Natural Serotonin Stimulant for Appetite Suppression and Targeted Eating as an Alternative to Conventional Obesity Treatments
A Natural Serotonin Stimulant for Appetite Suppression and Targeted Eating as an Alternative to Conventional Obesity Treatments Open
Recent advances in obesity management reflect the integration of pharmacological, surgical, and behavioral interventions, yet sustainable long-term outcomes remain elusive due to high attrition rates, the complexity of protocol compliance …
View article: The professional identity of STEM faculty as instructors of course-based research experiences
The professional identity of STEM faculty as instructors of course-based research experiences Open
The professional identity of scientists has historically been cultivated to value research over teaching, which can undermine initiatives that aim to reform science education. Course-Based Research Experiences (CRE) and the inclusive Resea…
View article: A-177 Using the Building Research Establishment Environmental Assessment Method to maximise the Environmental Social Governance benefits of building a pathology hub laboratory
A-177 Using the Building Research Establishment Environmental Assessment Method to maximise the Environmental Social Governance benefits of building a pathology hub laboratory Open
Background Organisations focusing on Environmental Social Governance (ESG) factors do not just benefit society and the planet, they also enjoy better performance across key profitability metrics, driven by higher growth and productivity, l…
View article: Developing a High Velocity Dataset Quality Checking Pipeline
Developing a High Velocity Dataset Quality Checking Pipeline Open
ObjectiveThe volume and frequency of refreshed data within the [organisation] has increased significantly since the beginning of the COVID-19 pandemic. Therefore, a more efficient data quality (DQ) checking process was necessary. Approach …
View article: An inclusive Research and Education Community (iREC) model to facilitate undergraduate science education reform
An inclusive Research and Education Community (iREC) model to facilitate undergraduate science education reform Open
Over the last two decades, there have been numerous initiatives to improve undergraduate student outcomes in STEM. One model for scalable reform is the inclusive Research Education Community (iREC). In an iREC, STEM faculty from colleges a…
View article: Characterization of mycobacteriophage Adephagia cytotoxic proteins
Characterization of mycobacteriophage Adephagia cytotoxic proteins Open
Mycobacterium phage Adephagia is a cluster K phage that infects Mycobacterium smegmatis and some strains of Mycobacterium pathogens. Adephagia has a siphoviral virion morphology and is temperate. Its genome is 59,646 bp long and codes for …
View article: Investigating Novel<i>Streptomyces</i>Bacteriophage Endolysins as Potential Antimicrobial Agents
Investigating Novel<i>Streptomyces</i>Bacteriophage Endolysins as Potential Antimicrobial Agents Open
Background As antibiotic resistance has become a major global threat; the World Health Organization (WHO) has urgently called for alternative strategies for control of bacterial infections. Endolysin, a phage-encoded protein, can degrade b…
View article: Models of classroom assessment for course-based research experiences
Models of classroom assessment for course-based research experiences Open
Course-based research pedagogy involves positioning students as contributors to authentic research projects as part of an engaging educational experience that promotes their learning and persistence in science. To develop a model for asses…
View article: Complete Genome Sequences of the Novel Cluster BP Phages Infecting Streptomyces sanglieri, AxeJC, Cumberbatch, Eastland, Eklok, HFrancette, Ignacio, Piccadilly, and Vondra
Complete Genome Sequences of the Novel Cluster BP Phages Infecting Streptomyces sanglieri, AxeJC, Cumberbatch, Eastland, Eklok, HFrancette, Ignacio, Piccadilly, and Vondra Open
The Streptomyces sanglieri bacteriophages AxeJC, Cumberbatch, Eastland, Eklok, HFrancette, Ignacio, Piccadilly, and Vondra form a novel actinobacteriophage cluster, BP. These siphoviruses have circularly permuted genomes with an average si…
View article: Instructional Models for Course-Based Research Experience (CRE) Teaching
Instructional Models for Course-Based Research Experience (CRE) Teaching Open
A report on research that explicates three models of pedagogical practice that underpin and characterize inquiry instruction in a course-based research experience.
View article: Crowdsourcing biocuration: The Community Assessment of Community Annotation with Ontologies (CACAO)
Crowdsourcing biocuration: The Community Assessment of Community Annotation with Ontologies (CACAO) Open
Experimental data about gene functions curated from the primary literature have enormous value for research scientists in understanding biology. Using the Gene Ontology (GO), manual curation by experts has provided an important resource fo…
View article: Crowdsourcing biocuration: the Community Assessment of Community Annotation with Ontologies (CACAO)
Crowdsourcing biocuration: the Community Assessment of Community Annotation with Ontologies (CACAO) Open
Experimental data about known gene functions curated from the primary literature have enormous value for research scientists in understanding biology. Using the Gene Ontology (GO), manual curation by experts has provided an important resou…
View article: Genomic diversity of bacteriophages infecting Microbacterium spp
Genomic diversity of bacteriophages infecting Microbacterium spp Open
The bacteriophage population is vast, dynamic, old, and genetically diverse. The genomics of phages that infect bacterial hosts in the phylum Actinobacteria show them to not only be diverse but also pervasively mosaic, and replete with gen…
View article: Student and Faculty Tools for Generating High-Quality Phage Annotations
Student and Faculty Tools for Generating High-Quality Phage Annotations Open
Phage genome annotation requires collaboration between faculty and their student research team. This set of resources provides tools to assist both students and faculty in the evaluation and submission of a complete phage genome.
View article: Understanding Gene Prediction Programs
Understanding Gene Prediction Programs Open
Introduces students to the key biological concepts underlying computational gene prediction. Outcomes include ability to analyze and evaluate output of gene prediction programs, and reflect on their engagement with big data and computation…
View article: Exploring patient preferences for involvement in medication management in hospitals
Exploring patient preferences for involvement in medication management in hospitals Open
Aim The aim of this study was to identify patient preferences for involvement in medication management during hospitalization. Design A qualitative descriptive study. Methods This is a study of 20 inpatients in two medical and two surgical…
View article: Complete Genome Sequences of 44 <i>Arthrobacter</i> Phages
Complete Genome Sequences of 44 <i>Arthrobacter</i> Phages Open
We report here the complete genome sequences of 44 phages infecting Arthrobacter sp. strain ATCC 21022. These phages have double-stranded DNA genomes with sizes ranging from 15,680 to 70,707 bp and G+C contents from 45.1% to 68.5%. All thr…
View article: Eight Genome Sequences of Cluster BE1 Phages That Infect <i>Streptomyces</i> Species
Eight Genome Sequences of Cluster BE1 Phages That Infect <i>Streptomyces</i> Species Open
Cluster BE1 Streptomyces bacteriophages belong to the Siphoviridae , with genome sizes over 130 kbp, and they contain direct terminal repeats of approximately 11 kbp. Eight newly isolated closely related cluster BE1 phages contain 43 to 48…
View article: Results of a large-of-entry study: The prevalence of drug-and polysubstance-preloading
Results of a large-of-entry study: The prevalence of drug-and polysubstance-preloading Open
Introduction and Aims\nPreloading is a cultural phenomenon that has gained notoriety within the substance use literature. Although a plethora of research has investigated alcohol‐preloading, a reliable study exploring the prevalence and co…
View article: Development, Validation, and Application of the Microbiology Concept Inventory
Development, Validation, and Application of the Microbiology Concept Inventory Open
If we are to teach effectively, tools are needed to measure student learning. A widely used method for quickly measuring student understanding of core concepts in a discipline is the concept inventory (CI). Using the American Society for M…
View article: Concept Inventory Development Reveals Common Student Misconceptions about Microbiology
Concept Inventory Development Reveals Common Student Misconceptions about Microbiology Open
Misconceptions, or alternative conceptions, are incorrect understandings that students have incorporated into their prior knowledge. The goal of this study was the identification of misconceptions in microbiology held by undergraduate stud…
View article: Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages
Tales of diversity: Genomic and morphological characteristics of forty-six Arthrobacter phages Open
The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantia…
View article: Genome Sequence of Mycobacterium Phage CrystalP
Genome Sequence of Mycobacterium Phage CrystalP Open
Mycobacteriophage CrystalP is a newly isolated phage infecting Mycobacterium smegmatis strain mc 2 155. CrystalP has a 76,483-bp genome and is predicted to contain 143 protein-coding and 2 tRNA genes, including repressor and integrase gene…
View article: Genome Sequences of Five <i>Streptomyces</i> Bacteriophages Forming Cluster BG
Genome Sequences of Five <i>Streptomyces</i> Bacteriophages Forming Cluster BG Open
Cluster BG of the actinobacteriophage was formed upon discovery of five novel bacteriophages isolated by enrichment from their host, Streptomyces griseus subsp. griseus strain ATCC 10137. Four members of this cluster (BabyGotBac, Maih, TP1…
View article: Genome Sequences of <i>Streptomyces</i> Phages Amela and Verse
Genome Sequences of <i>Streptomyces</i> Phages Amela and Verse Open
Amela and Verse are two Streptomyces phages isolated by enrichment on Streptomyces venezuelae (ATCC 10712) from two different soil samples. Amela has a genome length of 49,452, with 75 genes. Verse has a genome length of 49,483, with 75 ge…
View article: Comparison of medication policies to guide nursing practice across seven Victorian health services
Comparison of medication policies to guide nursing practice across seven Victorian health services Open
Objectives The objective of this paper is to review and compare the content of medication management policies across seven Australian health services located in the state of Victoria. Methods The medication management policies for health p…
View article: Comparative Genomics of Cluster O Mycobacteriophages
Comparative Genomics of Cluster O Mycobacteriophages Open
Mycobacteriophages--viruses of mycobacterial hosts--are genetically diverse but morphologically are all classified in the Caudovirales with double-stranded DNA and tails. We describe here a group of five closely related mycobacteriophages-…