Leif Steil
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View article: The <i>S. aureus</i> serine protease-like protein B (SplB) is a potent allergen causing eosinophilic airway inflammation in mice
The <i>S. aureus</i> serine protease-like protein B (SplB) is a potent allergen causing eosinophilic airway inflammation in mice Open
Asthma is a chronic inflammatory disease associated with increased Staphylococcus aureus airway colonization. However, the causal relationship is unknown. S. aureus secretes several virulence factors that promote allergic sensitization. Th…
View article: Identification of novel human cellular substrates of Staphylococcus aureus serine protease SplB
Identification of novel human cellular substrates of Staphylococcus aureus serine protease SplB Open
Staphylococcus aureus colonizes up to one third of the human population yet retains the capacity to cause invasive, life-threatening infections. The growing prevalence of antimicrobial resistance further complicates treatment. A major cont…
View article: Integrated genomic and proteomic analysis of the mouse-adapted <i>Staphylococcus aureus</i> strain JSNZ
Integrated genomic and proteomic analysis of the mouse-adapted <i>Staphylococcus aureus</i> strain JSNZ Open
Mouse-adapted Staphylococcus aureus strains have become increasingly relevant in infection research thanks to their ability to better recapitulate clinical infection dynamics in mouse models. However, detailed characterisations required to…
View article: TIE-UP-SIN: a novel method for enhanced identification of protein–protein interactions
TIE-UP-SIN: a novel method for enhanced identification of protein–protein interactions Open
Proteins function through complex interaction networks that govern nearly all aspects of cellular physiology. Identifying protein–protein interactions (PPIs) under native conditions remains challenging due to the transient nature of many c…
View article: The global proteome of Streptococcus pneumoniae EF3030 under nutrient-defined in vitro conditions
The global proteome of Streptococcus pneumoniae EF3030 under nutrient-defined in vitro conditions Open
Introduction Streptococcus pneumoniae is a human pathobiont that asymptomatically colonizes the upper respiratory tract but can cause severe diseases such as pneumonia, sepsis, and meningitis, as well as non-invasive infections like otitis…
View article: Reprogramming of bacterial virulence by lysine acetylation
Reprogramming of bacterial virulence by lysine acetylation Open
Gram-negative bacteria use a plethora of virulence factors to infect eukaryotic cells. CE-clan protease-related virulence factors were reported to act as deubiquitinases/ubiquitin-like specific proteases. Some have an additional acetyltran…
View article: pTripleTREP – A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus
pTripleTREP – A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus Open
View article: The global proteome of <i>Streptococcus pneumoniae</i> EF3030 under nutrient-defined <i>in vitro</i> conditions
The global proteome of <i>Streptococcus pneumoniae</i> EF3030 under nutrient-defined <i>in vitro</i> conditions Open
Streptococcus pneumoniae is a pathobiont that colonises the upper respiratory tract of humans without causing symptoms but can cause a range of life-threatening diseases including pneumonia, sepsis, and meningitis. It also causes less seve…
View article: Platelet-activating histone/antihistone IgG complexes in anti-PF4–negative thrombosis and thrombocytopenia syndrome
Platelet-activating histone/antihistone IgG complexes in anti-PF4–negative thrombosis and thrombocytopenia syndrome Open
Thrombosis and thrombocytopenia syndromes (TTS) describe immune-mediated thrombotic adverse reactions after vaccination against COVID-19. Vaccine-induced immune thrombotic thrombocytopenia (VITT) is a well-known subentity of TTS, caused by…
View article: Staphylococcal SplA and SplB serine proteases target ubiquitin(-like) specific proteases
Staphylococcal SplA and SplB serine proteases target ubiquitin(-like) specific proteases Open
Staphylococcus aureus is a Gram-positive opportunistic pathogen that has colonized nearly 30% of the human population and can cause life-threatening infections. S. aureus exports a variety of virulence factors, such as a novel set of extra…
View article: SpectroPipeR—a streamlining post Spectronaut® DIA-MS data analysis R package
SpectroPipeR—a streamlining post Spectronaut® DIA-MS data analysis R package Open
Summary Proteome studies frequently encounter challenges in down-stream data analysis due to limited bioinformatics resources, rapid data generation, and variations in analytical methods. To address these issues, we developed SpectroPipeR,…
View article: Biochemical analyses of cystatin-C dimers and cathepsin-B reveals a trypsin-driven feedback mechanism in acute pancreatitis
Biochemical analyses of cystatin-C dimers and cathepsin-B reveals a trypsin-driven feedback mechanism in acute pancreatitis Open
View article: A De Novo Dominant-Negative PSMB8 Mutation Causes Severe CANDLE/PRAAS Due to Arrested Proteasome Biogenesis
A De Novo Dominant-Negative PSMB8 Mutation Causes Severe CANDLE/PRAAS Due to Arrested Proteasome Biogenesis Open
View article: Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ
Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ Open
View article: Molecular and structural basis of oligopeptide recognition by the Ami transporter system in pneumococci
Molecular and structural basis of oligopeptide recognition by the Ami transporter system in pneumococci Open
ATP-binding cassette (ABC) transport systems are crucial for bacteria to ensure sufficient uptake of nutrients that are not produced de novo or improve the energy balance. The cell surface of the pathobiont Streptococcus pneumoniae (pneumo…
View article: The extracellular serine protease from Staphylococcus epidermidis elicits a type 2-biased immune response in atopic dermatitis patients
The extracellular serine protease from Staphylococcus epidermidis elicits a type 2-biased immune response in atopic dermatitis patients Open
Background Atopic dermatitis (AD) is a chronic, relapsing inflammatory skin disease with skin barrier defects and a misdirected type 2 immune response against harmless antigens. The skin microbiome in AD is characterized by a reduction in …
View article: Molecular and Structural Basis of Oligopeptide Recognition and Transport by the Ami Transporter System in Pneumococci
Molecular and Structural Basis of Oligopeptide Recognition and Transport by the Ami Transporter System in Pneumococci Open
ATP-binding cassette (ABC) transport systems are crucial for bacteria to ensure sufficient uptake of nutrients that are not produced de novo or improve the energy balance. The cell surface of the pathobiont Streptococcus pneumoniae (pneumo…
View article: Enhancing bacterial fitness and recombinant enzyme yield by engineering the quality control protease HtrA of <i>Bacillus subtilis</i>
Enhancing bacterial fitness and recombinant enzyme yield by engineering the quality control protease HtrA of <i>Bacillus subtilis</i> Open
Bacillus subtilis is a Gram-positive bacterium that is well-known for its high secretory capacity. Although secretion of homologous proteins is extremely efficient, secretion of heterologous proteins imposes what is known as “secretion str…
View article: MassSpecPreppy—An end‐to‐end solution for automated protein concentration determination and flexible sample digestion for proteomics applications
MassSpecPreppy—An end‐to‐end solution for automated protein concentration determination and flexible sample digestion for proteomics applications Open
In proteomics, fast, efficient, and highly reproducible sample preparation is of utmost importance, particularly in view of fast scanning mass spectrometers enabling analyses of large sample series. To address this need, we have developed …
View article: MassSpecPreppy - an end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications
MassSpecPreppy - an end-to-end solution for automated protein concentration determination and flexible sample digestion for proteomics applications Open
In proteomics, fast, efficient and highly reproducible sample preparation is of utmost importance, particularly in view of fast scanning mass spectrometers enabling analyses of large sample series. To address this need, we have developed t…
View article: Double trouble: Bacillus depends on a functional Tat machinery to avoid severe oxidative stress and starvation upon entry into a NaCl-depleted environment
Double trouble: Bacillus depends on a functional Tat machinery to avoid severe oxidative stress and starvation upon entry into a NaCl-depleted environment Open
View article: Impact of high salinity and the compatible solute glycine betaine on gene expression of <i>Bacillus subtilis</i>
Impact of high salinity and the compatible solute glycine betaine on gene expression of <i>Bacillus subtilis</i> Open
Summary The Gram‐positive bacterium Bacillus subtilis is frequently exposed to hyperosmotic conditions. In addition to the induction of genes involved in the accumulation of compatible solutes, high salinity exerts widespread effects on B.…
View article: Pathogen-specific antibody profiles in patients with severe systemic infections
Pathogen-specific antibody profiles in patients with severe systemic infections Open
Infections are often caused by pathobionts, endogenous bacteria that belong to the microbiota. Trauma and surgical intervention can allow bacteria to overcome host defences, ultimately leading to sepsis if left untreated. One of the main d…
View article: Author Correction: Proteomic and Transcriptomic Changes in Hibernating Grizzly Bears Reveal Metabolic and Signaling Pathways that Protect against Muscle Atrophy
Author Correction: Proteomic and Transcriptomic Changes in Hibernating Grizzly Bears Reveal Metabolic and Signaling Pathways that Protect against Muscle Atrophy Open
View article: Proteomic and Transcriptomic Changes in Hibernating Grizzly Bears Reveal Metabolic and Signaling Pathways that Protect against Muscle Atrophy
Proteomic and Transcriptomic Changes in Hibernating Grizzly Bears Reveal Metabolic and Signaling Pathways that Protect against Muscle Atrophy Open
View article: Improved Wound Healing of Airway Epithelial Cells Is Mediated by Cold Atmospheric Plasma: A Time Course-Related Proteome Analysis
Improved Wound Healing of Airway Epithelial Cells Is Mediated by Cold Atmospheric Plasma: A Time Course-Related Proteome Analysis Open
The promising potential of cold atmospheric plasma (CAP) treatment as a new therapeutic option in the field of medicine, particularly in Otorhinolaryngology and Respiratory medicine, demands primarily the assessment of potential risks and …
View article: Regulation of Biofilm Aging and Dispersal in <i>Bacillus subtilis</i> by the Alternative Sigma Factor SigB
Regulation of Biofilm Aging and Dispersal in <i>Bacillus subtilis</i> by the Alternative Sigma Factor SigB Open
Biofilms are crucial for bacterial survival, adaptation, and dissemination in natural, industrial, and medical systems. Sessile cells embedded in the self-produced extracellular matrix of the biofilm benefit from a division of labor and ar…
View article: Toward the Relevance of Platelet Subpopulations for Transfusion Medicine
Toward the Relevance of Platelet Subpopulations for Transfusion Medicine Open
Circulating platelets consist of subpopulations with different age, maturation state and size. In this review, we address the association between platelet size and platelet function and summarize the current knowledge on platelet subpopula…
View article: Large-scale reduction of the <i>Bacillus subtilis</i> genome: consequences for the transcriptional network, resource allocation, and metabolism
Large-scale reduction of the <i>Bacillus subtilis</i> genome: consequences for the transcriptional network, resource allocation, and metabolism Open
Understanding cellular life requires a comprehensive knowledge of the essential cellular functions, the components involved, and their interactions. Minimized genomes are an important tool to gain this knowledge. We have constructed strain…
View article: Changes of myocardial gene expression and protein composition in patients with dilated cardiomyopathy after immunoadsorption with subsequent immunoglobulin substitution
Changes of myocardial gene expression and protein composition in patients with dilated cardiomyopathy after immunoadsorption with subsequent immunoglobulin substitution Open