Leonard Medrano
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View article: Highly stable and immunogenic CMV T cell vaccine candidate developed using a synthetic MVA platform
Highly stable and immunogenic CMV T cell vaccine candidate developed using a synthetic MVA platform Open
Human cytomegalovirus (CMV) is the most common infectious cause of complications post-transplantation, while a CMV vaccine for transplant recipients has yet to be licensed. Triplex, a multiantigen Modified Vaccinia Ankara (MVA)-vectored CM…
View article: Microwell culture platform maintains viability and mass of human pancreatic islets
Microwell culture platform maintains viability and mass of human pancreatic islets Open
Background Transplantation of the human pancreatic islets is a promising approach for specific types of diabetes to improve glycemic control. Although effective, there are several issues that limit the clinical expansion of this treatment,…
View article: A unique adipocyte progenitor population promotes age-related adiposity
A unique adipocyte progenitor population promotes age-related adiposity Open
The average fat mass in adults increases dramatically with age, and older people often suffer from visceral obesity and related adverse metabolic disorders. Unfortunately, how aging leads to fat accumulation is poorly understood. It is kno…
View article: A Multiparametric Assessment of Human Islets Predicts Transplant Outcomes in Diabetic Mice
A Multiparametric Assessment of Human Islets Predicts Transplant Outcomes in Diabetic Mice Open
Prior to transplantation into individuals with type 1 diabetes, in vitro assays are used to evaluate the quality, function and survival of isolated human islets. In addition to the assessments of these parameters in islet, they can be eval…
View article: Semi-Automated Assessment of Human Islet Viability Predicts Transplantation Outcomes in a Diabetic Mouse Model
Semi-Automated Assessment of Human Islet Viability Predicts Transplantation Outcomes in a Diabetic Mouse Model Open
In clinical and experimental human pancreatic islet transplantations, establishing pretransplant assessments that accurately predict transplantation outcomes is crucial. Conventional in vitro viability assessment that relies on manual coun…
View article: Low- and high-thermogenic brown adipocyte subpopulations coexist in murine adipose tissue
Low- and high-thermogenic brown adipocyte subpopulations coexist in murine adipose tissue Open
Brown adipose tissue (BAT), as the main site of adaptive thermogenesis, exerts beneficial metabolic effects on obesity and insulin resistance. BAT has been previously assumed to contain a homogeneous population of brown adipocytes. Utilizi…
View article: Oxygen transporter for the hypoxic transplantation site
Oxygen transporter for the hypoxic transplantation site Open
Cell transplantation is a promising treatment for complementing lost function by replacing new cells with a desired function, e.g. pancreatic islet transplantation for diabetics. To prevent cell obliteration, oxygen supply is critical afte…
View article: Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata
Evaluation of collagenase gold plus BP protease in isolating islets from human pancreata Open
Selection of enzymes for optimal pancreas digestion is essential for successful human islet isolations. The aim of this study was to evaluate the efficacy and outcome of using Collagenase Gold plus BP protease (VitaCyte) (n = 8) by compari…
View article: Oxygen environment and islet size are the primary limiting factors of isolated pancreatic islet survival
Oxygen environment and islet size are the primary limiting factors of isolated pancreatic islet survival Open
Hypoxic core conditions, induced by the oxygen gradient inside islets, contribute to the development of central necrosis of human isolated islets. Supplying sufficient oxygen during culture could be an effective and reasonable method to ma…