L. Andrew Lee
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View article: Automated optimization of resin selection, wash parameters, and elution parameters for protein A-based purifications
Automated optimization of resin selection, wash parameters, and elution parameters for protein A-based purifications Open
Automated protein purification is essential for meeting the growing demands of high-throughput sample processing capabilities, especially when screening multiple parameters. For instance, protein A-based affinity chromatography is an impor…
View article: The Algorithm and The Abode: Automation of Architectural Workflows
The Algorithm and The Abode: Automation of Architectural Workflows Open
This research explored the automation of digital workflows and computational software for the design and manufacture of prefabricated component housing. The thesis was conducted in collaboration with an industry connection, Otago-based hig…
View article: Menentukan Harga Kain Katun Menggunakan Fuzzy Inference System Metode Mamdani
Menentukan Harga Kain Katun Menggunakan Fuzzy Inference System Metode Mamdani Open
Entrepreneurs must keep up with technological developments to compete in an increasingly competitive business environment. This competition causes price fluctuations that affect sales, requiring the right decisions for business survival. C…
View article: Incorporation of automated buffer exchange empowers high-throughput protein and plasmid purification for downstream uses
Incorporation of automated buffer exchange empowers high-throughput protein and plasmid purification for downstream uses Open
The continued acceleration of time-to-market product development and rising demand for biotherapeutics have hastened the need for higher throughput within the biopharmaceutical industry. Automated liquid handlers (ALH) are increasingly pop…
View article: Purification, Characterization, and Structural Studies of a Sulfatase from Pedobacter yulinensis
Purification, Characterization, and Structural Studies of a Sulfatase from Pedobacter yulinensis Open
Sulfatases are ubiquitous enzymes that hydrolyze sulfate from sulfated organic substrates such as carbohydrates, steroids, and flavones. These enzymes can be exploited in the field of biotechnology to analyze sulfated metabolites in humans…
View article: Automated multi‐attribute method sample preparation using high‐throughput buffer exchange tips
Automated multi‐attribute method sample preparation using high‐throughput buffer exchange tips Open
Rationale The multi‐attribute method (MAM) has become a valuable mass spectrometry (MS)‐based tool that can identify and quantify the site‐specific product attributes and purity information for biotherapeutics such as monoclonal antibodies…
View article: Factors Compromising Glucuronidase Performance in Urine Drug Testing Potentially Resulting in False Negatives
Factors Compromising Glucuronidase Performance in Urine Drug Testing Potentially Resulting in False Negatives Open
Next generation β-glucuronidases can effectively cleave glucuronides in urine at room temperature. However, during the discovery studies, additional challenges were identified for urine drug testing across biologically relevant pH extremes…
View article: Enhancing the multi-attribute method through an automated and high-throughput sample preparation
Enhancing the multi-attribute method through an automated and high-throughput sample preparation Open
The multi-attribute method (MAM), a recent advance in the application of liquid chromatography-mass spectrometry within the pharmaceutical industry, enables the simultaneous monitoring of multiple product quality attributes in a single ana…
View article: Parallel Sample Processing using Dispersive INtip Micro-purification on Programmable Multichannel Pipettes
Parallel Sample Processing using Dispersive INtip Micro-purification on Programmable Multichannel Pipettes Open
Automation gives researchers the ability to process and screen orders of magnitude higher numbers of samples than manual experimentation. Current biomacromolecule separation methodologies suffer from necessary manual intervention, making t…
View article: Degradation of Opioids and Opiates During Acid Hydrolysis Leads to Reduced Recovery Compared to Enzymatic Hydrolysis
Degradation of Opioids and Opiates During Acid Hydrolysis Leads to Reduced Recovery Compared to Enzymatic Hydrolysis Open
Drug monitoring laboratories utilize a hydrolysis process to liberate the opiates from their glucuronide conjugates to facilitate their detection by tandem mass spectrometry (MS). Both acid and enzyme hydrolysis have been reported as viabl…