Leslie T. Buck
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View article: Thianthrenium Salt Mediated Homologative 1,3-Dielectrophilic Activation Strategy for Alkene Difunctionalization
Thianthrenium Salt Mediated Homologative 1,3-Dielectrophilic Activation Strategy for Alkene Difunctionalization Open
The chemical introduction of heteroatoms via alkene difunctionalization (e.g., dihydroxylation, diamination, dihalogenation) is a traditional route to convert abundant olefins into highly functionalized building blocks. While the transform…
View article: Thianthrenium Salt‐Mediated Homologative 1,3‐Dielectrophilic Activation Strategy for Alkene Difunctionalization
Thianthrenium Salt‐Mediated Homologative 1,3‐Dielectrophilic Activation Strategy for Alkene Difunctionalization Open
The chemical introduction of heteroatoms via alkene difunctionalization (e.g., dihydroxylation, diamination, dihalogenation) is a traditional route to convert abundant olefins into highly functionalized building blocks. While the transform…
View article: Cover Feature: Thianthrenium Salt Mediated Homologative 1,3‐Dielectrophilic Activation Strategy for Alkene Difunctionalization
Cover Feature: Thianthrenium Salt Mediated Homologative 1,3‐Dielectrophilic Activation Strategy for Alkene Difunctionalization Open
View article: Increased GABAA receptor open probability: Adaptive mechanisms to cope with anoxia in the painted turtle
Increased GABAA receptor open probability: Adaptive mechanisms to cope with anoxia in the painted turtle Open
View article: Cultured primary turtle hepatocytes: a cellular model for the study of temperature and anoxia
Cultured primary turtle hepatocytes: a cellular model for the study of temperature and anoxia Open
We have optimized a methodology for two-dimensional (2-D) culturing of primary western painted turtle hepatocytes and used this model to study the effects of cyanide and temperature on structural rearrangement, and the effect of cyanide on…
View article: Alfaxalone is an effective anesthetic for the electrophysiological study of anoxia-tolerance mechanisms in western painted turtle pyramidal neurons
Alfaxalone is an effective anesthetic for the electrophysiological study of anoxia-tolerance mechanisms in western painted turtle pyramidal neurons Open
Anoxia in the mammalian brain leads to hyper-excitability and cell death; however, this cascade of events does not occur in the anoxia-tolerant brain of the western painted turtle, Chrysemys picta belli . The painted turtle has become an i…
View article: A58 UTILIZATION OF HYPOXIA-TOLERANT ORGANISMS AS A MODEL IN THE STUDY OF ISCHEMIC-REPERFUSION INJURY IN TRANSPLANT HEPATOLOGY
A58 UTILIZATION OF HYPOXIA-TOLERANT ORGANISMS AS A MODEL IN THE STUDY OF ISCHEMIC-REPERFUSION INJURY IN TRANSPLANT HEPATOLOGY Open
Background Ischemic-reperfusion injury (IRI) is a barrier to successful liver transplantation. This complex process is initiated by an episode of hypoxia and decreased adenosine triphosphate (ATP) production. Mammalian hepatocytes are susc…
View article: Alfaxalone does not have long‐term effects on goldfish pyramidal neuron action potential properties or <scp> GABA <sub>A</sub> </scp> receptor currents
Alfaxalone does not have long‐term effects on goldfish pyramidal neuron action potential properties or <span> GABA <sub>A</sub> </span> receptor currents Open
Anesthetics have varying physiological effects, but most notably alter ion channel kinetics. Alfaxalone is a rapid induction and washout neuroactive anesthetic, which potentiates γ‐aminobutyric acid (GABA)‐activated GABA A receptor (GABA A…
View article: Evolution of a novel regulatory mechanism of hypoxia inducible factor in hypoxia-tolerant electric fishes
Evolution of a novel regulatory mechanism of hypoxia inducible factor in hypoxia-tolerant electric fishes Open
Hypoxia is a significant source of metabolic stress that activates many cellular pathways involved in cellular differentiation, proliferation, and cell death. Hypoxia is also a major component in many human diseases and a known driver of m…
View article: Alfaxalone is an Effective Anesthetic for the Electrophysiological Study of Anoxia-Tolerance Mechanisms in Western Painted Turtle Pyramidal Neurons v1
Alfaxalone is an Effective Anesthetic for the Electrophysiological Study of Anoxia-Tolerance Mechanisms in Western Painted Turtle Pyramidal Neurons v1 Open
Many anesthetics have long-term effects on ion channels and are not appropriate for same day experimentation. Using whole-cell electrophysiological techniques, we examine the effects of the anesthetic, Alfaxalone, on pyramidal cell action …
View article: Skeletal muscle metabolism and contraction performance regulation by teneurin C-terminal-associated peptide-1
Skeletal muscle metabolism and contraction performance regulation by teneurin C-terminal-associated peptide-1 Open
Skeletal muscle regulation is responsible for voluntary muscular movement in vertebrates. The genes of two essential proteins, teneurins and latrophilins (LPHN), evolving in ancestors of multicellular animals form a ligand-receptor pair, a…
View article: Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin action
Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin action Open
Skeletal muscle regulation is responsible for voluntary muscular movement in vertebrates. The genes of two essential proteins, teneurins and latrophilins (LPHN), evolving in ancestors of multicellular animals, form a ligand-receptor pair, …
View article: Exposure to low temperature prepares the turtle brain to withstand anoxic environments during overwintering
Exposure to low temperature prepares the turtle brain to withstand anoxic environments during overwintering Open
In most vertebrates, anoxia drastically reduces the production of the essential adenosine triphosphate (ATP) to power its many necessary functions, and, consequently, cell death occurs within minutes. However, some vertebrates, such as the…
View article: Cytoskeletal Arrest: An Anoxia Tolerance Mechanism
Cytoskeletal Arrest: An Anoxia Tolerance Mechanism Open
Polymerization of actin filaments and microtubules constitutes a ubiquitous demand for cellular adenosine-5′-triphosphate (ATP) and guanosine-5′-triphosphate (GTP). In anoxia-tolerant animals, ATP consumption is minimized during overwinter…
View article: Scavenging of reactive oxygen species mimics the anoxic response in goldfish pyramidal neurons
Scavenging of reactive oxygen species mimics the anoxic response in goldfish pyramidal neurons Open
Goldfish are one of a few species able to avoid cellular damage during month-long periods in severely hypoxic environments. By suppressing action potentials in excitatory glutamatergic neurons, the goldfish brain decreases its overall ener…
View article: GABA receptor inhibition and severe hypoxia induce a paroxysmal depolarization shift in goldfish neurons
GABA receptor inhibition and severe hypoxia induce a paroxysmal depolarization shift in goldfish neurons Open
This work shows that the combination of anoxia and inhibition of GABA receptors induces seizure-like activities in goldfish telencephalic pyramidal and stellate neurons. Importantly, to prevent seizure-like activity, an intact GABA-mediate…
View article: Oxygen-sensitive interneurons exhibit increased activity and GABA release during ROS scavenging in the cerebral cortex of the western painted turtle
Oxygen-sensitive interneurons exhibit increased activity and GABA release during ROS scavenging in the cerebral cortex of the western painted turtle Open
The western painted turtle ( Chrysemys picta bellii) has the unique ability of surviving several months in the absence of oxygen, which is termed anoxia. One major protective strategy that the turtle employs during anoxia is a reduction in…
View article: Mitochondrial matrix <scp>pH</scp> acidifies during anoxia and is maintained by the F<sub>1</sub>F<sub>o</sub>‐<scp>ATP</scp>ase in anoxia‐tolerant painted turtle cortical neurons
Mitochondrial matrix <span>pH</span> acidifies during anoxia and is maintained by the F<sub>1</sub>F<sub>o</sub>‐<span>ATP</span>ase in anoxia‐tolerant painted turtle cortical neurons Open
The western painted turtle ( Chrysemys picta bellii ) can survive extended periods of anoxia via a series of mechanisms that serve to reduce its energetic needs. Central to these mechanisms is the response of mitochondria, which depolarize…
View article: Taurine activates glycine and GABAA receptor currents in anoxia-tolerant painted turtle pyramidal neurons
Taurine activates glycine and GABAA receptor currents in anoxia-tolerant painted turtle pyramidal neurons Open
Unlike anoxia-intolerant mammals, painted turtles can survive extended periods without oxygen. This is partly accomplished by an anoxia-mediated increase in gamma-aminobutyric acid (GABA) release, which activates GABA receptors and mediate…
View article: Characterization of Differentiated SH-SY5Y as Neuronal Screening Model Reveals Increased Oxidative Vulnerability
Characterization of Differentiated SH-SY5Y as Neuronal Screening Model Reveals Increased Oxidative Vulnerability Open
View article: Stellate and pyramidal neurons in goldfish telencephalon respond differently to anoxia and GABA receptor inhibition
Stellate and pyramidal neurons in goldfish telencephalon respond differently to anoxia and GABA receptor inhibition Open
With oxygen deprivation, the mammalian brain undergoes hyper-activity and neuronal death while this does not occur in the anoxia tolerant goldfish (Carassius auratus). Anoxic survival of the goldfish may rely on neuromodulatory mechanisms …
View article: Transcriptomic Responses of the Heart and Brain to Anoxia in the Western Painted Turtle
Transcriptomic Responses of the Heart and Brain to Anoxia in the Western Painted Turtle Open
Painted turtles are the most anoxia-tolerant tetrapods known, capable of surviving without oxygen for more than four months at 3°C and 30 hours at 20°C. To investigate the transcriptomic basis of this ability, we used RNA-seq to quantify m…
View article: Decreases in mitochondrial reactive oxygen species initiate GABA<sub>A</sub> receptor‐mediated electrical suppression in anoxia‐tolerant turtle neurons
Decreases in mitochondrial reactive oxygen species initiate GABA<sub>A</sub> receptor‐mediated electrical suppression in anoxia‐tolerant turtle neurons Open
Key points Anoxia induces hyper‐excitability and cell death in mammalian brain but in the western painted turtle ( Chrysemys picta bellii ) enhanced GABA transmission prevents injury. The mechanism responsible for increased GABA transmissi…
View article: c_picta_human_orthologs_with_id_prefix
c_picta_human_orthologs_with_id_prefix Open
C. picta human orthologs with IDs. Supplementary material to: Transcriptomic responses of the heart and brain to anoxia in the western painted turtle Authors Sarah W. Keenan, Craig A. Hill, Cyriac Kandoth, Leslie T. Buck and Daniel E. Warr…
View article: mm_cpicta3_gpipe_predictions.gft
mm_cpicta3_gpipe_predictions.gft Open
C. picta gene predictions. Supplementary material to: Transcriptomic responses of the heart and brain to anoxia in the western painted turtle Authors Sarah W. Keenan, Craig A. Hill, Cyriac Kandoth, Leslie T. Buck and Daniel E. Warren PLoS …