Letizia Barbieri
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View article: Intracellular Binding of Novel Fluorinated Compounds to Carbonic Anhydrase Isoforms Explored by In-Cell <sup>19</sup> F NMR
Intracellular Binding of Novel Fluorinated Compounds to Carbonic Anhydrase Isoforms Explored by In-Cell <sup>19</sup> F NMR Open
One of the most important challenges of drug development is identifying whether a candidate molecule can efficiently reach and engage intracellular targets. Cell-based assays are usually employed to address this issue; nevertheless, many c…
View article: Protein structure and interactions elucidated with in-cell NMR for different cell cycle phases and in 3D human tissue models
Protein structure and interactions elucidated with in-cell NMR for different cell cycle phases and in 3D human tissue models Open
Most of our knowledge of protein structure and function originates from experiments performed with purified proteins resuspended in dilute, buffered solutions. However, most proteins function in crowded intracellular environments with comp…
View article: Towards cost-effective side-chain isotope labelling of proteins expressed in human cells
Towards cost-effective side-chain isotope labelling of proteins expressed in human cells Open
View article: Inducible protein expression in stably transfected cells paves the way toward in-cell NMR studies in defined physiological states and 3D tissue cultures.
Inducible protein expression in stably transfected cells paves the way toward in-cell NMR studies in defined physiological states and 3D tissue cultures. Open
In-cell NMR spectroscopy is the sole technique for characterizing protein structure, dynamics, and interactions in living human cells at atomic resolution. However, its applications have been restricted to asynchronous single-cell suspensi…
View article: Inducible protein expression in stably transfected cells paves the way toward in-cell NMR studies in defined physiological states and 3D tissue cultures.
Inducible protein expression in stably transfected cells paves the way toward in-cell NMR studies in defined physiological states and 3D tissue cultures. Open
In-cell NMR spectroscopy is the sole technique for characterizing protein structure, dynamics, and interactions in living human cells at atomic resolution. However, its applications have been restricted to asynchronous single-cell suspensi…
View article: Controlling the incorporation of fluorinated amino acids in human cells and its structural impact
Controlling the incorporation of fluorinated amino acids in human cells and its structural impact Open
Fluorinated aromatic amino acids (FAAs) are promising tools when studying protein structure and dynamics by NMR spectroscopy. The incorporation FAAs in mammalian expression systems has been introduced only recently. Here, we investigate th…
View article: Ligand-Based Competition Binding by Real-Time <sup>19</sup>F NMR in Human Cells
Ligand-Based Competition Binding by Real-Time <sup>19</sup>F NMR in Human Cells Open
The development of more effective drugs requires knowledge of their bioavailability and binding efficacy directly in the native cellular environment. In-cell nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for investigatin…
View article: The synthesis of specifically isotope labelled fluorotryptophan and its use in mammalian cell-based protein expression for <sup>19</sup>F-NMR applications
The synthesis of specifically isotope labelled fluorotryptophan and its use in mammalian cell-based protein expression for <sup>19</sup>F-NMR applications Open
Combining multistep organic synthesis with mammalian cell based protein overexpression leads to isolated 13 C– 19 F spin systems in tryptophan side chains, which represent ideal sensors to probe protein interaction and dynamics using NMR s…
View article: Direct Expression of Fluorinated Proteins in Human Cells for <sup>19</sup>F In-Cell NMR Spectroscopy
Direct Expression of Fluorinated Proteins in Human Cells for <sup>19</sup>F In-Cell NMR Spectroscopy Open
In-cell NMR spectroscopy is a powerful approach to study protein structure and function in the native cellular environment. It provides precious insights into the folding, maturation, interactions, and ligand binding of important pharmacol…
View article: Protein delivery to living cells by thermal stimulation for biophysical investigation
Protein delivery to living cells by thermal stimulation for biophysical investigation Open
View article: Determination of intracellular protein–ligand binding affinity by competition binding in-cell NMR
Determination of intracellular protein–ligand binding affinity by competition binding in-cell NMR Open
Structure-based drug development suffers from high attrition rates due to the poor activity of lead compounds in cellular and animal models caused by low cell penetrance, off-target binding or changes in the conformation of the target prot…
View article: Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor
Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor Open
In-cell NMR is a unique approach to observe the structural and dynamic properties of biological macromolecules at atomic resolution directly in living cells. Protein folding, chemical modifications, and conformational changes induced by li…
View article: Protein in-cell NMR spectroscopy at 1.2 GHz
Protein in-cell NMR spectroscopy at 1.2 GHz Open
In-cell NMR spectroscopy provides precious structural and functional information on biological macromolecules in their native cellular environment at atomic resolution. However, the intrinsic low sensitivity of NMR imposes a big limitation…
View article: Intracellular Binding/Unbinding Kinetics of Approved Drugs to Carbonic Anhydrase II Observed by in-Cell NMR
Intracellular Binding/Unbinding Kinetics of Approved Drugs to Carbonic Anhydrase II Observed by in-Cell NMR Open
Candidate drugs rationally designed in vitro often fail due to low efficacy in vivo caused by low tissue availability or because of unwanted side effects. To overcome the limitations of in vitro rational drug design, t…
View article: Real-Time Quantitative In-Cell NMR: Ligand Binding and Protein Oxidation Monitored in Human Cells Using Multivariate Curve Resolution
Real-Time Quantitative In-Cell NMR: Ligand Binding and Protein Oxidation Monitored in Human Cells Using Multivariate Curve Resolution Open
In-cell NMR can investigate protein conformational changes at atomic resolution, such as those changes induced by drug binding or chemical modifications, directly in living human cells, and therefore has great potential in the context of d…
View article: Drug Screening in Human Cells by NMR Spectroscopy Allows the Early Assessment of Drug Potency
Drug Screening in Human Cells by NMR Spectroscopy Allows the Early Assessment of Drug Potency Open
Structure‐based drug development is often hampered by the lack of in vivo activity of promising compounds screened in vitro, due to low membrane permeability or poor intracellular binding selectivity. Herein, we show that ligand screening …
View article: Drug Screening in Human Cells by NMR Spectroscopy Allows the Early Assessment of Drug Potency
Drug Screening in Human Cells by NMR Spectroscopy Allows the Early Assessment of Drug Potency Open
Structure‐based drug development is often hampered by the lack of in vivo activity of promising compounds screened in vitro, due to low membrane permeability or poor intracellular binding selectivity. Herein, we show that ligand screening …
View article: Biomolecular NMR at 1.2 GHz
Biomolecular NMR at 1.2 GHz Open
The development of new superconducting ceramic materials, which maintain the superconductivity at very intense magnetic fields, has prompted the development of a new generation of highly homogeneous high field magnets that has trespassed t…
View article: Backbone resonance assignment of human DJ-1 in the reduced state and in the cysteine sulfinic acid state
Backbone resonance assignment of human DJ-1 in the reduced state and in the cysteine sulfinic acid state Open
View article: Inflammatory, Serological and Vascular Determinants of Cardiovascular Disease in Systemic Lupus Erythematosus Patients
Inflammatory, Serological and Vascular Determinants of Cardiovascular Disease in Systemic Lupus Erythematosus Patients Open
Background and aim: Systemic lupus erythematosus (SLE) is associated with increased risk of cardiovascular disease (CVD). Among many mechanisms, accelerated atherosclerosis, endothelial dysfunction, and hypercoagulability play a main role.…
View article: Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions
Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions Open
View article: The cysteine-reactive small molecule ebselen facilitates effective SOD1 maturation
The cysteine-reactive small molecule ebselen facilitates effective SOD1 maturation Open
View article: <sup>15</sup>N isotopic labelling for in-cell protein studies by NMR spectroscopy and single-cell IR synchrotron radiation FTIR microscopy: a correlative study
<sup>15</sup>N isotopic labelling for in-cell protein studies by NMR spectroscopy and single-cell IR synchrotron radiation FTIR microscopy: a correlative study Open
The effect of15N-enrichment on human cells analyzed by correlative in-cell NMR and single-cell SR-FTIR experiments.
View article: Intracellular metal binding and redox behavior of human DJ-1
Intracellular metal binding and redox behavior of human DJ-1 Open
View article: A molecular chaperone activity of CCS restores the maturation of SOD1 fALS mutants
A molecular chaperone activity of CCS restores the maturation of SOD1 fALS mutants Open
Superoxide dismutase 1 (SOD1) is an important metalloprotein for cellular oxidative stress defence, that is mutated in familiar variants of Amyotrophic Lateral Sclerosis (fALS). Some mutations destabilize the apo protein, leading to the fo…
View article: Severe Hypothyroidism due to the Loss of Therapeutic Efficacy of l-Thyroxine in a Patient with Esophageal Complication Associated with Systemic Sclerosis
Severe Hypothyroidism due to the Loss of Therapeutic Efficacy of l-Thyroxine in a Patient with Esophageal Complication Associated with Systemic Sclerosis Open
A recognition of a poor absorption is crucial in patients with hypothyroidism and SSc to reduce the risk of the subsequent adverse events. This case suggests the importance of clinical and laboratory surveillance in patients with SSc and H…
View article: Importance of sarcopenia parameter changes after living donor liver transplantation
Importance of sarcopenia parameter changes after living donor liver transplantation Open
The systemic role of muscle tissue is strengthened by the large system of hormones, chemokines and other mediators that constitute a dense network of communication between the skeletal muscle and the liver (1,2).
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View article: Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells
Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells Open
View article: Portal Hypertension Model in Pigs
Portal Hypertension Model in Pigs Open
View article: Direct structural evidence of protein redox regulation obtained by in-cell NMR
Direct structural evidence of protein redox regulation obtained by in-cell NMR Open