Li‐En Hsieh
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View article: Nanogram of Arg 1 Delivered by extracellular vesicles restore ARG1 activity in a mouse model of ARG1-D and improve lifespan
Nanogram of Arg 1 Delivered by extracellular vesicles restore ARG1 activity in a mouse model of ARG1-D and improve lifespan Open
Arginase 1 (ARG1) deficiency (ARG1-D) is a rare genetic disorder due to loss of ARG1, the final enzyme in the urea cycle. ARG1-D hepatocytes are impaired in converting arginine into urea, resulting in elevated peripheral arginine and ammon…
View article: Multivalent Exosome Based Protein Vaccine: A “Mix and Match” Approach to Epidemic Viruses’ Challenges
Multivalent Exosome Based Protein Vaccine: A “Mix and Match” Approach to Epidemic Viruses’ Challenges Open
Background: Endemic viruses are becoming increasingly the norm, and the development of a rapid and effective vaccine is necessary. Methods: Here, we used our StealthXTM exosome platform to express either Influenza H3 (StealthXTM-Hemaggluti…
View article: Multivalent Exosome based protein vaccine: a “mix and match” approach to epidemic viruses’ challenges
Multivalent Exosome based protein vaccine: a “mix and match” approach to epidemic viruses’ challenges Open
Endemic viruses are becoming increasingly the norm, and the development of a rapid and effective vaccine is emergent. Here, we used our StealthX ™ exosome platform to express either Influenza H3 (Stealth™ X-Hemagglutinin, STX-H3) or SARS-C…
View article: T Cells in Multisystem Inflammatory Syndrome in Children (MIS-C) Have a Predominant CD4+ T Helper Response to SARS-CoV-2 Peptides and Numerous Virus-Specific CD4− CD8− Double-Negative T Cells
T Cells in Multisystem Inflammatory Syndrome in Children (MIS-C) Have a Predominant CD4+ T Helper Response to SARS-CoV-2 Peptides and Numerous Virus-Specific CD4− CD8− Double-Negative T Cells Open
We studied SARS-CoV-2-specific T cell responses in 22 subacute MIS-C children enrolled in 2021 and 2022 using peptide pools derived from SARS-CoV-2 spike or nonspike proteins. CD4+ and CD8+ SARS-CoV-2-specific T cells were detected in 5 su…
View article: Intravenous immunoglobulin induces IgG internalization by tolerogenic myeloid dendritic cells that secrete IL-10 and expand Fc-specific regulatory T cells
Intravenous immunoglobulin induces IgG internalization by tolerogenic myeloid dendritic cells that secrete IL-10 and expand Fc-specific regulatory T cells Open
Intravenous immunoglobulin (IVIG) is used as an immunomodulatory agent in many inflammatory conditions including Multisystem Inflammatory Syndrome-Children (MIS-C) and Kawasaki disease (KD). However, the exact mechanisms underlying its ant…
View article: SARS-CoV-2-specific T cell responses and immune regulation in infected pregnant women
SARS-CoV-2-specific T cell responses and immune regulation in infected pregnant women Open
We studied the T cell response to SARS-CoV-2 spike and non-spike peptide epitopes in eight convalescent pregnant women together with the immune monitoring that included innate tolerogenic dendritic cell populations important to maintain th…
View article: Characterization of SARS‐CoV‐2 and common cold coronavirus‐specific T‐cell responses in MIS‐C and Kawasaki disease children
Characterization of SARS‐CoV‐2 and common cold coronavirus‐specific T‐cell responses in MIS‐C and Kawasaki disease children Open
The immunopathogenesis of multisystem inflammatory syndrome (MIS‐C) in children that may follow exposure to SARS‐CoV‐2 is incompletely understood. Here, we studied SARS‐CoV‐2‐specific T cells in MIS‐C, Kawasaki disease (KD), and SARS‐CoV‐2…
View article: Characterization of the T Cell Response to Lactobacillus casei Cell Wall Extract in Children With Kawasaki Disease and Its Potential Role in Vascular Inflammation
Characterization of the T Cell Response to Lactobacillus casei Cell Wall Extract in Children With Kawasaki Disease and Its Potential Role in Vascular Inflammation Open
KD is an acute febrile illness and systemic vasculitis of unknown etiology among young children, which can cause coronary artery abnormalities and aneurysms (CAA) and is the leading cause of acquired heart disease among children in the US.…
View article: IgG Epitopes Processed and Presented by IgG+ B Cells Induce Suppression by Human Thymic-Derived Regulatory T Cells
IgG Epitopes Processed and Presented by IgG+ B Cells Induce Suppression by Human Thymic-Derived Regulatory T Cells Open
We described a human regulatory T cell (Treg) population activated by IgG+ B cells presenting peptides of the heavy C region (Fc) via processing of the surface IgG underlying a model for B cell–Treg cooperation in the human immune regulati…
View article: Characterization of circulating immune cells in acute Kawasaki disease suggests exposure to different antigens
Characterization of circulating immune cells in acute Kawasaki disease suggests exposure to different antigens Open
Summary Kawasaki disease (KD) is an acute pediatric vasculitis of unknown etiology that can cause coronary artery aneurysms, and is the leading cause of acquired heart disease in children. We studied aspects of the innate and adaptive immu…
View article: Development of Human‐Derived Cell Culture Lines for Recurrent Respiratory Papillomatosis
Development of Human‐Derived Cell Culture Lines for Recurrent Respiratory Papillomatosis Open
Recurrent respiratory papillomatosis (RRP) is mainly caused by human papillomavirus (HPV) 6 and 11. While various adjuvant therapies have been reported, no effective therapy has been documented to universally “cure” this disease. In the er…
View article: Pediatric tolerogenic DCs expressing CD4 and immunoglobulin‐like transcript receptor (ILT)‐4 secrete IL‐10 in response to Fc and adenosine
Pediatric tolerogenic DCs expressing CD4 and immunoglobulin‐like transcript receptor (ILT)‐4 secrete IL‐10 in response to Fc and adenosine Open
We characterized a novel population of tolerogenic myeloid dendritic cells (tmDCs) defined as CD11c + CD11b + CD14 + CD4 + and immunoglobulin‐like transcript receptor (ILT)‐4 + that are significantly more abundant in the circulation of inf…