Liam P. Keegan
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View article: Editing-independent effects of Adar in <i>Drosophila melanogaster</i>
Editing-independent effects of Adar in <i>Drosophila melanogaster</i> Open
Vertebrate ADAR RNA editing enzymes prevent cellular dsRNA from aberrantly activating antiviral dsRNA sensors. ADARs inhibit antiviral sensor activation by deaminating selected adenosines to inosines in dsRNA and by engaging in inhibitory …
View article: ADAR1: Beyond Just an RNA Editor
ADAR1: Beyond Just an RNA Editor Open
The RNA editing enzyme adenosine deaminase acting on RNA 1 (ADAR1) has recently emerged from relative obscurity to be recognized as a key player in a variety of inflammatory diseases, including cancer. This growing recognition has generate…
View article: Global analysis by LC-MS/MS of <i>N6</i> -methyladenosine and inosine in mRNA reveal complex incidence
Global analysis by LC-MS/MS of <i>N6</i> -methyladenosine and inosine in mRNA reveal complex incidence Open
The precise and unambiguous detection and quantification of internal RNA modifications represents a critical step for understanding their physiological functions. The methods of direct RNA sequencing are quickly developing allowing for the…
View article: Distinct interactomes of ADAR1 nuclear and cytoplasmic protein isoforms and their responses to interferon induction
Distinct interactomes of ADAR1 nuclear and cytoplasmic protein isoforms and their responses to interferon induction Open
The RNA editing enzyme adenosine deaminase acting on RNA 1 (ADAR1) is essential for correct functioning of innate immune responses. The ADAR1p110 isoform is mainly nuclear and ADAR1p150, which is interferon (IFN) inducible, is predominatel…
View article: Overexpression of Egr1 Transcription Regulator Contributes to Schwann Cell Differentiation Defects in Neural Crest-Specific Adar1 Knockout Mice
Overexpression of Egr1 Transcription Regulator Contributes to Schwann Cell Differentiation Defects in Neural Crest-Specific Adar1 Knockout Mice Open
Adenosine deaminase acting on RNA 1 (ADAR1) is the principal enzyme for the adenosine-to-inosine RNA editing that prevents the aberrant activation of cytosolic nucleic acid sensors by endogenous double stranded RNAs and the activation of i…
View article: Global analysis by LC-MS/MS of<i>N6</i>-methyladenosine and inosine in mRNA reveal complex incidence
Global analysis by LC-MS/MS of<i>N6</i>-methyladenosine and inosine in mRNA reveal complex incidence Open
The precise and unambiguous detection and quantification of internal RNA modifications represents a critical step for understanding their physiological functions. The methods of direct RNA sequencing are quickly developing allowing for the…
View article: An ADAR1 dsRBD3-PKR kinase domain interaction on dsRNA inhibits PKR activation
An ADAR1 dsRBD3-PKR kinase domain interaction on dsRNA inhibits PKR activation Open
Adar null mutant mouse embryos die with aberrant double-stranded RNA (dsRNA)-driven interferon induction, and Adar Mavs double mutants, in which interferon induction is prevented, die soon after birth. Protein kinase R (Pkr) is aberrantly …
View article: Amyloid precursor protein induces reactive astrogliosis
Amyloid precursor protein induces reactive astrogliosis Open
Aim Astrocytes respond to stressors by acquiring a reactive state characterized by changes in their morphology and function. Molecules underlying reactive astrogliosis, however, remain largely unknown. Given that several studies observed i…
View article: Amyloid precursor protein induces reactive astrogliosis
Amyloid precursor protein induces reactive astrogliosis Open
We present in vitro and in vivo evidence demonstrating that Amyloid Precursor Protein (APP) acts as an essential instigator of reactive astrogliosis. Cell-specific overexpression of APP in cultured astrocytes led to remodelling of the inte…
View article: WITHDRAWN: Changes in ADAR1 activity during Plasmodium infection contribute to protection from malaria
WITHDRAWN: Changes in ADAR1 activity during Plasmodium infection contribute to protection from malaria Open
Withdrawal Statement The authors have withdrawn this manuscript because they have a new manuscript that will include only part of the data presented here. Therefore, the authors do not wish this work to be cited as reference for the projec…
View article: Adenosine Deaminase Acting on RNA (ADAR) Enzymes: A Journey from Weird to Wondrous
Adenosine Deaminase Acting on RNA (ADAR) Enzymes: A Journey from Weird to Wondrous Open
The adenosine deaminase acting on RNA (ADAR) enzymes that catalyze the conversion of adenosine to inosine in double-stranded (ds)RNA are evolutionarily conserved and are essential for many biological functions including nervous system func…
View article: Aberrant activation of the innate immune sensor PKR by self dsRNA is prevented by direct interaction with ADAR1
Aberrant activation of the innate immune sensor PKR by self dsRNA is prevented by direct interaction with ADAR1 Open
Summary Loss of dsRNA editing by Adar1 leads to aberrant interferon induction in Adar null mouse embryos. Adar Mavs mutants, in which this interferon induction is prevented, die within two weeks of birth. We show here that early death of p…
View article: ADAR2 enzymes: efficient site-specific RNA editors with gene therapy aspirations
ADAR2 enzymes: efficient site-specific RNA editors with gene therapy aspirations Open
The adenosine deaminase acting on RNA (ADAR) enzymes are essential for neuronal function and innate immune control. ADAR1 RNA editing prevents aberrant activation of antiviral dsRNA sensors through editing of long, double-stranded RNAs (ds…
View article: Spatial modeling reveals nuclear phosphorylation and subcellular shuttling of YAP upon drug-induced liver injury
Spatial modeling reveals nuclear phosphorylation and subcellular shuttling of YAP upon drug-induced liver injury Open
The Hippo signaling pathway controls cell proliferation and tissue regeneration via its transcriptional effectors yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ). In this context, the canonical pat…
View article: European COST action CA16120 EPITRAN: the first four years and beyond
European COST action CA16120 EPITRAN: the first four years and beyond Open
Do not go where the path may lead, go instead where there is no path and leave a trail. ~ Ralph Waldo EmersonFigure 1. Henri Grosjean enjoying a beer with a friendHow do you measure the success of ...
View article: Interplays of different types of epitranscriptomic mRNA modifications
Interplays of different types of epitranscriptomic mRNA modifications Open
Eukaryotic mRNAs are modified by several chemical marks which have significant impacts on mRNA biology, gene expression, and cellular metabolism as well as on the survival and development of the whole organism. The most abundant and well-s…
View article: The effects of RNA editing in cancer tissue at different stages in carcinogenesis
The effects of RNA editing in cancer tissue at different stages in carcinogenesis Open
RNA editing is one of the most prevalent and abundant forms of post-transcriptional RNA modification observed in normal physiological processes and often aberrant in diseases including cancer. RNA editing changes the sequences of mRNAs, ma…
View article: Biallelic variants in <i>ADARB1</i>, encoding a dsRNA-specific adenosine deaminase, cause a severe developmental and epileptic encephalopathy
Biallelic variants in <i>ADARB1</i>, encoding a dsRNA-specific adenosine deaminase, cause a severe developmental and epileptic encephalopathy Open
Background Adenosine-to-inosine RNA editing is a co-transcriptional/post-transcriptional modification of double-stranded RNA, catalysed by one of two active adenosine deaminases acting on RNA (ADARs), ADAR1 and ADAR2. ADARB1 encodes the en…
View article: Additional file 6 of Membrane and synaptic defects leading to neurodegeneration in Adar mutant Drosophila are rescued by increased autophagy
Additional file 6 of Membrane and synaptic defects leading to neurodegeneration in Adar mutant Drosophila are rescued by increased autophagy Open
Additional file 6. Excel sheet containing source data file for Figure 1A ,1B ,1C.1D ,4A,4D,4E,6A,6F,6G,6H and 6I.
View article: Additional file 6 of Membrane and synaptic defects leading to neurodegeneration in Adar mutant Drosophila are rescued by increased autophagy
Additional file 6 of Membrane and synaptic defects leading to neurodegeneration in Adar mutant Drosophila are rescued by increased autophagy Open
Additional file 6. Excel sheet containing source data file for Figure 1A ,1B ,1C.1D ,4A,4D,4E,6A,6F,6G,6H and 6I.
View article: Activation of innate immunity by mitochondrial dsRNA in mouse cells lacking p53 protein
Activation of innate immunity by mitochondrial dsRNA in mouse cells lacking p53 protein Open
Viral and cellular double-stranded RNA (dsRNA) is recognized by cytosolic innate immune sensors, including RIG-I-like receptors. Some cytoplasmic dsRNA is commonly present in cells, and one source is mitochondrial dsRNA, which results from…
View article: The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs
The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs Open
The Sp1 transcription factor has been defined as glutamine-rich activator. The Nine amino acid TransActivation Domains (9aaTAD) have been identified in numerous transcription activators. Here, we identified the conserved 9aaTAD motif in th…
View article: ADAR RNA editing below the backbone
ADAR RNA editing below the backbone Open
ADAR RNA editing enzymes ( a denosine d e a minases acting on R NA) that convert adenosine bases to inosines were first identified biochemically 30 years ago. Since then, studies on ADARs in genetic model organisms, and evolutionary compar…
View article: A to I editing in disease is not fake news
A to I editing in disease is not fake news Open
Adenosine deaminases acting on RNA (ADARs) are zinc-containing enzymes that deaminate adenosine bases to inosines within dsRNA regions in transcripts. In short, structured dsRNA hairpins individual adenosine bases may be targeted specifica…