Liangbiao Chen
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View article: Benchmarking metabolic RNA labeling techniques for high-throughput single-cell RNA sequencing
Benchmarking metabolic RNA labeling techniques for high-throughput single-cell RNA sequencing Open
Metabolic RNA labeling with high-throughput single-cell RNA sequencing (scRNA-seq) enables precise measurement of gene expression dynamics in complex biological processes, such as cell state transitions and embryogenesis. This technique, w…
View article: Single‐Cell Transcriptome Profiling Reveals Conserved IFNγ‐IL8 Signaling‐Induced Antibacterial Neutrophil States during Bacterial Infection
Single‐Cell Transcriptome Profiling Reveals Conserved IFNγ‐IL8 Signaling‐Induced Antibacterial Neutrophil States during Bacterial Infection Open
Streptococcus agalactiae is a significant pathogen in both humans and animals, yet the immune cell subtype dynamics during infection remain poorly defined. Leveraging the high susceptibility and tractability of Nile tilapia ( Oreochromis n…
View article: Hepcidin Deficiency Disrupts Iron Homeostasis and Induces Ferroptosis in Zebrafish Liver
Hepcidin Deficiency Disrupts Iron Homeostasis and Induces Ferroptosis in Zebrafish Liver Open
Hepcidin is a key regulator of systemic iron homeostasis, which is essential for maintaining iron balance and cellular health. To investigate its role in zebrafish, we empolyed a hepcidin knockout model. Morphological and histological anal…
View article: Heat inducible nuclear translocation of Kdm6bb drives temperature dependent sex reversal in Nile tilapia
Heat inducible nuclear translocation of Kdm6bb drives temperature dependent sex reversal in Nile tilapia Open
Understanding the primary molecular events driving temperature-dependent sex reversal (TSR) has proven challenging, particularly in distinguishing these from secondary effects of sexual differentiation. The mechanisms translating temperatu…
View article: Protocol for isolating nuclei from frozen fish heart tissue for single-cell genomic assays
Protocol for isolating nuclei from frozen fish heart tissue for single-cell genomic assays Open
Unbiased isolation of intact single nuclei from complex tissues, such as cryopreserved hearts, for massively parallel single-cell genomic assays is particularly challenging. Here, we present a protocol for isolating high-quality nuclei fro…
View article: Functional study of RNA alternative splicing in zebrafish adaptation to low temperature
Functional study of RNA alternative splicing in zebrafish adaptation to low temperature Open
View article: Comprehensive Analysis of Rna-Seq Data Reveals Complex Transcript Patterns of Takifugu Obscurus Under Cold Stress
Comprehensive Analysis of Rna-Seq Data Reveals Complex Transcript Patterns of Takifugu Obscurus Under Cold Stress Open
View article: Comprehensive Analysis of Mirna and Mrna Reveals the Transcriptional Regulatory Mechanism of Spotted Sea Bass (Lateolabrax Maculatus) During Overwintering
Comprehensive Analysis of Mirna and Mrna Reveals the Transcriptional Regulatory Mechanism of Spotted Sea Bass (Lateolabrax Maculatus) During Overwintering Open
View article: Gene expression and histological observations indicate kdm6bb regulates thermal tolerance in Nile tilapia
Gene expression and histological observations indicate kdm6bb regulates thermal tolerance in Nile tilapia Open
View article: A Novel Psychrotolerant Beneficial Bacterium Isolated from Antarctic Fish Enhances Disease Resistance in Aquaculture
A Novel Psychrotolerant Beneficial Bacterium Isolated from Antarctic Fish Enhances Disease Resistance in Aquaculture Open
View article: Functional study of RNA alternative splicing in zebrafish adaptation to low temperature
Functional study of RNA alternative splicing in zebrafish adaptation to low temperature Open
View article: Optimized protocol for nuclei isolation from aquatic fish brain tissue for single-cell Genomic assays
Optimized protocol for nuclei isolation from aquatic fish brain tissue for single-cell Genomic assays Open
View article: Adaptive evolution for freshwater colonization in Coilia nasus by directional selection on osmoregulation genes
Adaptive evolution for freshwater colonization in Coilia nasus by directional selection on osmoregulation genes Open
View article: Identification of two miRNAs regulating cardiomyocyte proliferation in an Antarctic icefish
Identification of two miRNAs regulating cardiomyocyte proliferation in an Antarctic icefish Open
View article: Differential tissue immune stimulation through immersion in bacterial and viral agonists in the Antarctic Notothenia rossii
Differential tissue immune stimulation through immersion in bacterial and viral agonists in the Antarctic Notothenia rossii Open
The genome evolution of Antarctic notothenioids has been modulated by their extreme environment over millennia and more recently by human-caused constraints such as overfishing and climate change. Here we investigated the characteristics o…
View article: The First High-Quality Genome Assembly of Freshwater Pearl Mussel Sinohyriopsis cumingii: New Insights into Pearl Biomineralization
The First High-Quality Genome Assembly of Freshwater Pearl Mussel Sinohyriopsis cumingii: New Insights into Pearl Biomineralization Open
China leads the world in freshwater pearl production, an industry in which the triangle sail mussel (Sinohyriopsis cumingii) plays a pivotal role. In this paper, we report a high-quality chromosome-level genome assembly of S. cumingii with…
View article: Metabolomic-based analysis reveals bile acid-mediated ovarian failure induced by low temperature in zebrafish
Metabolomic-based analysis reveals bile acid-mediated ovarian failure induced by low temperature in zebrafish Open
As ectotherms, fish are highly sensitive to temperature fluctuations, which can profoundly impact their reproductive cycles. In this study, we investigated the fertility and histological characteristics of zebrafish ( Danio rerio) o…
View article: Gwas and Selective Sweep Analysis Reveal the Genetic Basis of Cold Tolerance in the Domesticated Pufferfish(Takifugu Obscurus)
Gwas and Selective Sweep Analysis Reveal the Genetic Basis of Cold Tolerance in the Domesticated Pufferfish(Takifugu Obscurus) Open
View article: Tissue-specific temperature dependence of RNA editing levels in zebrafish
Tissue-specific temperature dependence of RNA editing levels in zebrafish Open
View article: Transcriptome analysis reveals the molecular mechanisms of heterosis on thermal resistance in hybrid tilapia (Oreochromi niloticus ♀×Oreochromi aureus ♂)
Transcriptome analysis reveals the molecular mechanisms of heterosis on thermal resistance in hybrid tilapia (Oreochromi niloticus ♀×Oreochromi aureus ♂) Open
Heterosis is a widely observed biological phenomenon in fishes, in which hybrids exhibit superior traits to their parents. However, the underlying molecular mechanisms for heterosis are not well understood. Tilapia is an economically impor…
View article: Assessment of Nonalcoholic Fatty Liver Disease Symptoms and Gut–Liver Axis Status in Zebrafish after Exposure to Polystyrene Microplastics and Oxytetracycline, Alone and in Combination
Assessment of Nonalcoholic Fatty Liver Disease Symptoms and Gut–Liver Axis Status in Zebrafish after Exposure to Polystyrene Microplastics and Oxytetracycline, Alone and in Combination Open
Our results suggested that exposure to MPs and OTC may disrupt the gut-liver axis and be associated with NAFLD occurrence. https://doi.org/10.1289/EHP11600.
View article: A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities
A symbiotic bacterium of Antarctic fish reveals environmental adaptability mechanisms and biosynthetic potential towards antibacterial and cytotoxic activities Open
Antarctic microbes are important agents for evolutionary adaptation and natural resource of bioactive compounds, harboring the particular metabolic pathways to biosynthesize natural products. However, not much is known on symbiotic microbi…
View article: Chromosome-level genome assembly of freshwater pearl mussel, Hyriopsis cumingii, provides insights into outstanding biomineralization ability
Chromosome-level genome assembly of freshwater pearl mussel, Hyriopsis cumingii, provides insights into outstanding biomineralization ability Open
China is the largest producer of pearls, accounted for over 90% of world’s total pearl production. The triangle sail mussel (Hyriopsis cumingii), the most important species for freshwater pearl culture in China, is a well-known mussel reco…
View article: <i>Dusp1</i> regulates thermal tolerance limits in zebrafish by maintaining mitochondrial integrity
<i>Dusp1</i> regulates thermal tolerance limits in zebrafish by maintaining mitochondrial integrity Open
Temperature tolerance restricts the distribution of a species. However, the molecular and cellular mechanisms that set the thermal tolerance limits of an organism are poorly understood. Here, we report on the function of dual-specificity p…
View article: Population genomics of an icefish reveals mechanisms of glacier-driven adaptive radiation in Antarctic notothenioids
Population genomics of an icefish reveals mechanisms of glacier-driven adaptive radiation in Antarctic notothenioids Open
View article: Transcriptome Analysis of Immune Response against Streptococcus agalactiae Infection in the Nile Tilapia GIFT Strain
Transcriptome Analysis of Immune Response against Streptococcus agalactiae Infection in the Nile Tilapia GIFT Strain Open
Streptococcus agalactiae (group B streptococcus, GBS), a broad-spectrum pathogen, causes great economic losses in fish aquaculture, especially the industry of tilapia. Until now, the knowledge of the immune response mechanism against S. ag…
View article: Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death
Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death Open
Acute hypoxia in water has always been a thorny problem in aquaculture. Oxygen and iron play important roles and are interdependent in fish. Iron is essential for oxygen transport and its concentration tightly controlled to maintain the ce…
View article: Reduced Hypoxia Tolerance and Altered Gill Morphology at Elevated Temperatures May Limit the Survival of Tilapia (GIFT, Oreochromis niloticus) under Global Warming
Reduced Hypoxia Tolerance and Altered Gill Morphology at Elevated Temperatures May Limit the Survival of Tilapia (GIFT, Oreochromis niloticus) under Global Warming Open
Nile tilapia (Oreochromis niloticus) is one of the most important food fishes in global aquaculture. The optimal rearing temperature for Nile tilapia is 27–30 °C; however, in some Asian breeding areas, such as south China, water temperatur…
View article: Transcriptomic and Phenotypic Analysis of CRISPR/Cas9-Mediated gluk2 Knockout in Zebrafish
Transcriptomic and Phenotypic Analysis of CRISPR/Cas9-Mediated gluk2 Knockout in Zebrafish Open
As a subtype of kainite receptors (KARs), GluK2 plays a role in the perception of cold in the periphery sensory neuron. However, the molecular mechanism for gluk2 on the cold stress in fish has not been reported. In this article, real-time…
View article: MiR-202-3p determines embryo viability during mid-blastula transition
MiR-202-3p determines embryo viability during mid-blastula transition Open
Developmental growth is an intricate process involving the coordinated regulation of the expression of various genes, and microRNAs (miRNAs) play crucial roles in diverse processes throughout animal development. The mid-blastula transition…