Lilian Vincis Pereira Sanglard
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View article: Benchmarking methods for genome annotation using Nanopore direct RNA in a non-model crop plant
Benchmarking methods for genome annotation using Nanopore direct RNA in a non-model crop plant Open
High-quality genome annotations are essential for transcriptomic analyses investigating plant responses to environmental stress. While Nanopore long-read direct RNA (dRNA) sequencing offers a powerful approach for improving genome annotati…
View article: Isolation of Pyrenophora teres f. teres from Infected Barley Leaves and Re-inoculation of Barley Leaves in a Glasshouse v1
Isolation of Pyrenophora teres f. teres from Infected Barley Leaves and Re-inoculation of Barley Leaves in a Glasshouse v1 Open
This protocol provides a detailed, step-by-step guide for the isolation of Pyrenophora teres f. teres (Ptt) from infected barley leaves, followed by culturing for vegetative growth, sporulation (conidiophore and conidia production), and in…
View article: Mitochondrial <i>atp1</i> mRNA knockdown by a custom-designed pentatricopeptide repeat protein alters ATP synthase
Mitochondrial <i>atp1</i> mRNA knockdown by a custom-designed pentatricopeptide repeat protein alters ATP synthase Open
Spontaneous mutations are rare in mitochondria and the lack of mitochondrial transformation methods has hindered genetic analyses. We show that a custom-designed RNA-binding pentatricopeptide repeat (PPR) protein binds and specifically ind…
View article: Alteration of Mitochondrial Transcript Expression in Arabidopsis thaliana Using a Custom-Made Library of Pentatricopeptide Repeat Proteins
Alteration of Mitochondrial Transcript Expression in Arabidopsis thaliana Using a Custom-Made Library of Pentatricopeptide Repeat Proteins Open
Pentatricopeptide repeat (PPR) proteins are considered a potential tool for manipulating organelle gene expression in plants because they can recognise a wide range of different RNA sequences, and the molecular basis for this sequence reco…
View article: Spatiotemporal patterns of wheat response to <i>Pyrenophora tritici-repentis</i> in asymptomatic regions revealed by transcriptomic and X-ray fluorescence microscopy analyses
Spatiotemporal patterns of wheat response to <i>Pyrenophora tritici-repentis</i> in asymptomatic regions revealed by transcriptomic and X-ray fluorescence microscopy analyses Open
Pathogen attacks elicit dynamic and widespread molecular responses in plants. While our understanding of plant responses has advanced considerably, little is known of the molecular responses in the asymptomatic ‘green’ regions adjoining le…
View article: Knockdown of mitochondrial<i>atp1</i>mRNA by a custom-designed pentatricopeptide repeat protein alters F<sub>1</sub>F<sub>o</sub>ATP synthase
Knockdown of mitochondrial<i>atp1</i>mRNA by a custom-designed pentatricopeptide repeat protein alters F<sub>1</sub>F<sub>o</sub>ATP synthase Open
We show that a custom-designed RNA-binding protein binds and specifically induces cleavage of atp1 RNA in mitochondria, significantly decreasing the abundance of the Atp1 protein and the assembled F 1 F o ATP synthase in Arabidopsis thalia…
View article: “Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy
“Wax On, Wax Off”: In Vivo Imaging of Plant Physiology and Disease with Fourier Transform Infrared Reflectance Microspectroscopy Open
Analysis of the epicuticular wax layer on the surface of plant leaves can provide a unique window into plant physiology and responses to environmental stimuli. Well‐established analytical methodologies can quantify epicuticular wax composi…
View article: The Pentatricopeptide Repeat Protein MEF100 Is Required for the Editing of Four Mitochondrial Editing Sites in Arabidopsis
The Pentatricopeptide Repeat Protein MEF100 Is Required for the Editing of Four Mitochondrial Editing Sites in Arabidopsis Open
In Arabidopsis thaliana there are more than 600 C-to-U RNA editing events in the mitochondria and at least 44 in the chloroplasts. Pentatricopeptide repeat (PPR) proteins provide the specificity for these reactions. They recognize RNA sequ…
View article: Synchrotron X-ray fluorescence microscopy-enabled elemental mapping illuminates the ‘battle for nutrients’ between plant and pathogen
Synchrotron X-ray fluorescence microscopy-enabled elemental mapping illuminates the ‘battle for nutrients’ between plant and pathogen Open
Metal homeostasis is integral to normal plant growth and development. During plant–pathogen interactions, the host and pathogen compete for the same nutrients, potentially impacting nutritional homeostasis. Our knowledge of outcome of the …
View article: A synthetic RNA editing factor edits its target site in chloroplasts and bacteria
A synthetic RNA editing factor edits its target site in chloroplasts and bacteria Open
Targeted cytidine to uridine RNA editing is a widespread phenomenon throughout the land plant lineage. Members of the pentatricopeptide repeat (PPR) protein family act as the specificity factors in this process. These proteins consist of h…
View article: Impaired Malate and Fumarate Accumulation Due to the Mutation of the Tonoplast Dicarboxylate Transporter Has Little Effects on Stomatal Behavior
Impaired Malate and Fumarate Accumulation Due to the Mutation of the Tonoplast Dicarboxylate Transporter Has Little Effects on Stomatal Behavior Open
Malate is a central metabolite involved in a multiplicity of plant metabolic pathways, being associated with mitochondrial metabolism and playing significant roles in stomatal movements. Vacuolar malate transport has been characterized at …
View article: Photosynthetic and metabolic acclimation to repeated drought events play key roles in drought tolerance in coffee
Photosynthetic and metabolic acclimation to repeated drought events play key roles in drought tolerance in coffee Open
Over the last decades, most information on the mechanisms underlying tolerance to drought has been gained by considering this stress as a single event that happens just once in the life of a plant, in contrast to what occurs under natural …
View article: The Pentatricopeptide Repeat Protein EMB2654 Is Essential for Trans-Splicing of a Chloroplast Small Ribosomal Subunit Transcript
The Pentatricopeptide Repeat Protein EMB2654 Is Essential for Trans-Splicing of a Chloroplast Small Ribosomal Subunit Transcript Open
We report the partial complementation and subsequent comparative molecular analysis of two nonviable mutants impaired in chloroplast translation, one (emb2394) lacking the RPL6 protein, and the other (emb2654) carrying a mutation in a gene…