Limiao Chen
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View article: CRISPR/Cas9-Mediated Targeted Mutagenesis of GmAS1/2 Genes Alters Leaf Shape in Soybean
CRISPR/Cas9-Mediated Targeted Mutagenesis of GmAS1/2 Genes Alters Leaf Shape in Soybean Open
ASYMMETRIC LEAVES1 (AS1) and AS2 play essential roles in regulating leaf development in plants. However, their functional roles in soybean remain poorly understood. Here, we identified two members of the soybean AS1 gene family, GmAS1a and…
View article: Regulatory Mechanism of the GmMYB14 Transcription Factor on Auxin-Related Proteins in Soybean
Regulatory Mechanism of the GmMYB14 Transcription Factor on Auxin-Related Proteins in Soybean Open
In a previous study, GmMYB14 overexpressing (GmMYB14-OX) transgenic soybean plants displayed a semi-dwarfism and compact phenotype, which was regulated by the brassinosteroid (BR) pathway. However, the phenotype of GmMYB14-OX plants could …
View article: The phosphatase CTDSPL2 promotes proliferation, invasion, metastasis and regorafenib resistance in osteosarcoma
The phosphatase CTDSPL2 promotes proliferation, invasion, metastasis and regorafenib resistance in osteosarcoma Open
Osteosarcoma is the most common bone malignancy in children and adolescents. Patients with metastatic and recurrent osteosarcoma have poor prognosis. Regorafenib is a multi-kinase inhibitor recommended as a complement to standard chemother…
View article: Screening and identification of salt tolerance soybean varieties and germplasms
Screening and identification of salt tolerance soybean varieties and germplasms Open
Soil salinization is a globally prevalent abiotic environmental stress. The imbalance of ions caused by high concentrations of sodium chloride results in a 40% reduction in soybean yield. Soybean, as an important crop for soil quality impr…
View article: Comparative genomic and transcriptomic analyses provide new insight into symbiotic host specificity
Comparative genomic and transcriptomic analyses provide new insight into symbiotic host specificity Open
Host specificity plays important roles in expanding the host range of rhizobia, while the genetic information responsible for host specificity remains largely unexplored. In this report, the roots of four symbiotic systems with notable dif…
View article: Investigating GmDWF1 in Soybean for Its Involvement in Brassinosteroid Synthesis and Plant Height Control
Investigating GmDWF1 in Soybean for Its Involvement in Brassinosteroid Synthesis and Plant Height Control Open
Soybeans have not adopted the Green Revolution, such as reduced height for increased planting density, which have proven beneficial for cereal crops. Our research identified the soybean genes GmDWF1aand GmDWF1b, homologous to Arabidopsis A…
View article: RNA-Seq and Comparative Transcriptomic Analyses of Asian Soybean Rust Resistant and Susceptible Soybean Genotypes Provide Insights into Identifying Disease Resistance Genes
RNA-Seq and Comparative Transcriptomic Analyses of Asian Soybean Rust Resistant and Susceptible Soybean Genotypes Provide Insights into Identifying Disease Resistance Genes Open
Asian soybean rust (ASR), caused by Phakopsora pachyrhizi, is one of the most destructive foliar diseases that affect soybeans. Developing resistant cultivars is the most cost-effective, environmentally friendly, and easy strategy for cont…
View article: Identification of Quantitative Trait Locus and Candidate Genes for Drought Tolerance in a Soybean Recombinant Inbred Line Population
Identification of Quantitative Trait Locus and Candidate Genes for Drought Tolerance in a Soybean Recombinant Inbred Line Population Open
With global warming and regional decreases in precipitation, drought has become a problem worldwide. As the number of arid regions in the world is increasing, drought has become a major factor leading to significant crop yield reductions a…
View article: CRISPR/Cas9-Mediated Targeted Mutagenesis of GmUGT Enhanced Soybean Resistance Against Leaf-Chewing Insects Through Flavonoids Biosynthesis
CRISPR/Cas9-Mediated Targeted Mutagenesis of GmUGT Enhanced Soybean Resistance Against Leaf-Chewing Insects Through Flavonoids Biosynthesis Open
Leaf-chewing insects are important pests that cause yield loss and reduce seed quality in soybeans ( Glycine max ). Breeding soybean varieties that are resistant to leaf-chewing insects can minimize the need for insecticide use and reduce …
View article: Experimental Innovations in Applied Chemistry under the “Emerging Engineering Education”: The Visible Light Catalytic Water Splitting for Hydrogen Production
Experimental Innovations in Applied Chemistry under the “Emerging Engineering Education”: The Visible Light Catalytic Water Splitting for Hydrogen Production Open
The scientific research of the preparation of ZrO2@TiO2 heterojunction and their catalytic properties for photocatalytic hydrogen evolution was designed as a comprehensive experiment for the students of applied chemistry major at Central S…
View article: Genome-Wide Identification and Characterization of Soybean GmLOR Gene Family and Expression Analysis in Response to Abiotic Stresses
Genome-Wide Identification and Characterization of Soybean GmLOR Gene Family and Expression Analysis in Response to Abiotic Stresses Open
The LOR (LURP-one related) family genes encode proteins containing a conserved LOR domain. Several members of the LOR family genes are required for defense against Hyaloperonospora parasitica (Hpa) in Arabidopsis. However, there are few re…
View article: Expression pattern of GmLAX genes under different stresses in soybean drought sensitive cultivar and tolerant cultivar
Expression pattern of GmLAX genes under different stresses in soybean drought sensitive cultivar and tolerant cultivar Open
Auxin has been reported to regulate plant growth and development, as well as to mediate plant adaption to abiotic stresses, including drought. AUX/LAX family displays auxin uptake functions and comprises four highly conserved genes AUX1 an…
View article: Whole-Genome Sequencing of Bradyrhizobium diazoefficiens 113-2 and Comparative Genomic Analysis Provide Molecular Insights Into Species Specificity and Host Specificity
Whole-Genome Sequencing of Bradyrhizobium diazoefficiens 113-2 and Comparative Genomic Analysis Provide Molecular Insights Into Species Specificity and Host Specificity Open
In the present study, we sequenced the complete genome of Bradyrhizobium diazoefficiens 113-2. The genomic characteristics of six selected rhizobial strains (two fast-growing rhizobia, two medium-slow-growing rhizobia and two slow-growing …
View article: Overexpression of <i>GmMYB14</i> improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway
Overexpression of <i>GmMYB14</i> improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway Open
Summary MYB transcription factors (TFs) have been reported to regulate the biosynthesis of secondary metabolites, as well as to mediate plant adaption to abiotic stresses, including drought. However, the roles of MYB TFs in regulating plan…
View article: A GATA Transcription Factor from Soybean (Glycine max) Regulates Chlorophyll Biosynthesis and Suppresses Growth in the Transgenic Arabidopsis thaliana
A GATA Transcription Factor from Soybean (Glycine max) Regulates Chlorophyll Biosynthesis and Suppresses Growth in the Transgenic Arabidopsis thaliana Open
Chlorophyll plays an essential role in photosynthetic light harvesting and energy transduction in green tissues of higher plants and is closely related to photosynthesis and crop yield. Identification of transcription factors (TFs) involve…
View article: Evaluation of seed vigor in soybean germplasms from different eco-regions
Evaluation of seed vigor in soybean germplasms from different eco-regions Open
Seed vigor plays an essential role in soybean production. Soybean seed is sensitive to environment and easy to get seed deterioration. Generally soybean seed vigor can be maintained for less than one year and have to be multiplied every ye…
View article: Overexpression of <i>GmWRI1b</i> in soybean stably improves plant architecture and associated yield parameters, and increases total seed oil production under field conditions
Overexpression of <i>GmWRI1b</i> in soybean stably improves plant architecture and associated yield parameters, and increases total seed oil production under field conditions Open
The functions of WRINKLED1 (WRI1) transcription factor in regulating fatty acid (FA) biosynthesis are highly conserved in crop plants, including maize (Zea mays; Pouvreau et al., 2011), rapeseed (Brassica napus; Li et al., 2015), oil palm …
View article: Additional file 5 of Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis
Additional file 5 of Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis Open
Additional file 5: Table S5. Primers for Glyma.04G190700-specific RNAi analysis.
View article: Additional file 4 of Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis
Additional file 4 of Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis Open
Additional file 4: Table S4. Primers for qPCR analysis of the 28 selected GmPLCPs.
View article: Additional file 3 of Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis
Additional file 3 of Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis Open
Additional file 3: Table S3. The gene ID information of the 42 GO terms in soybean PLCPs.
View article: Additional file 2 of Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis
Additional file 2 of Genome-wide survey of soybean papain-like cysteine proteases and their expression analysis in root nodule symbiosis Open
Additional file 2: Table S2. The conserved domain analysis of soybean PLCPs by NIH/NLM/NCBI CD-search tool.