Explanipedia

Recent advances in the prevention and treatment of respiratory syncytial virus disease Open

Alexandra Sánchez-Martínez, Tom Moore, T. Freitas, Tami R. Benzaken, Shaun O’Hagan , et al. · 2025

Respiratory syncytial virus (RSV) is associated with considerable healthcare burden; as such, prevention and treatment of RSV have long been considered a priority. Historic failures in RSV vaccine development had slowed the research field.…

Genomic Analysis and Surveillance of Respiratory Syncytial Virus Using Wastewater-Based Epidemiology Open

Danielle M Allen, Marina Reyne, Pearce Allingham, Ashley Levickas, Stephen H. Bell , et al. · 2024

Respiratory syncytial virus (RSV) causes severe infections in infants, immunocompromised or elderly individuals resulting in annual epidemics of respiratory disease. Currently, limited clinical surveillance and the lack of predictable seas…

Genomic Analysis and Surveillance of Respiratory Syncytial Virus (RSV) Using Wastewater-Based Epidemiology (WBE) Open

Danielle M. Allen, Marina Reyne, Pearce Allingham, Ashley Levickas, Stephen H. Bell , et al. · 2023

Respiratory syncytial virus (RSV) causes severe infections in infants, immunocompromised or elderly individuals resulting in annual epidemics of respiratory disease. Currently, limited clinical RSV surveillance and the lack of predictable …

Respiratory syncytial virus (RSV): over 60 years of research but still so many unanswered questions Open

Simon B. Drysdale, Lindsay Broadbent · 2023

An endogenously activated antiviral state restricts SARS-CoV-2 infection in differentiated primary airway epithelial cells Open

Lindsay Broadbent, Connor Bamford, Guillermo López–Campos, Sheerien Manzoor, David Courtney , et al. · 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of the coronavirus disease-19 (COVID-19) pandemic, was identified in late 2019 and caused >5 million deaths by February 2022. To date, targeted antiviral interventions…

Comparison of SARS-CoV-2 Evolution in Paediatric Primary Airway Epithelial Cell Cultures Compared with Vero-Derived Cell Lines Open

Connor Bamford, Lindsay Broadbent, Elihú Aranday-Cortés, Mary McCabe, James McKenna , et al. · 2022

SARS-CoV-2 can efficiently infect both children and adults, albeit with morbidity and mortality positively associated with increasing host age and presence of co-morbidities. SARS-CoV-2 continues to adapt to the human population, resulting…

Inhibition of the TLR4 signalling pathway with TAK-242 reduces RSV infection and cytokine release in primary airway epithelial cells Open

Lindsay Broadbent, Grace Roberts, Jonathon D. Coey, Judit Barabas, Michael D. Shields , et al. · 2021

Respiratory syncytial virus (RSV) infection is the leading cause of hospitalisation in children worldwide, but there is still no vaccine or anti-viral treatment available. RSV has been implicated in the development of respiratory diseases …

An Endogenously activated antiviral state restricts SARS-CoV-2 infection in differentiated primary airway epithelial cells Open

Lindsay Broadbent, Connor Bamford, Guillermo López–Campos, Sheerien Manzoor, David Courtney , et al. · 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of the coronavirus disease-19 (COVID-19) pandemic, was identified in late 2019 and went on to cause over 3.3 million deaths in 15 months. To date, targeted antiviral i…

Collaboration Between Host and Viral Factors Shape SARS-CoV-2 Evolution Open

Connor Bamford, Lindsay Broadbent, Elihú Aranday-Cortés, Mary McCabe, James McKenna , et al. · 2021

SARS-CoV-2 continues to evolve, resulting in several ‘variants of concern’ with novel properties. The factors driving SARS-CoV-2 fitness and evolution in the human respiratory tract remain poorly defined. Here, we provide evidence that bot…

Comparative primary paediatric nasal epithelial cell culture differentiation and RSV-induced cytopathogenesis following culture in two commercial media Open

Lindsay Broadbent, Sheerien Manzoor, Maria C. Zarcone, Judit Barabas, Michael D. Shields , et al. · 2020

The culture of differentiated human airway epithelial cells allows the study of pathogen-host interactions and innate immune responses in a physiologically relevant in vitro model. As the use of primary cell culture has gained popularity t…

The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins Open

Olivier Touzelet, Lindsay Broadbent, Stuart D. Armstrong, Waleed Aljabr, Elaine Cloutman-Green , et al. · 2020

Comparative primary paediatric nasal epithelial cell culture differentiation and RSV-induced cytopathogenesis following culture in two commercial media Open

Lindsay Broadbent, Sheerien Manzoor, Maria C. Zarcone, Judit Barabas, Mike Shields , et al. · 2020

The culture of differentiated human airway epithelial cells allows the study of pathogen-host interactions and innate immune responses in a physiologically relevant in vitro model. As the use of primary cell culture has gained popularity t…

Comparative Therapeutic Potential of ALX-0171 and Palivizumab against Respiratory Syncytial Virus Clinical Isolate Infection of Well-Differentiated Primary Pediatric Bronchial Epithelial Cell Cultures Open

Lindsay Broadbent, Hong Guo-Parke, Lyndsey J. Ferguson, Andrena Millar, Michael D. Shields , et al. · 2019

Respiratory syncytial virus (RSV) causes severe lower respiratory tract infections in young infants. There are no RSV-specific treatments available. Ablynx has been developing an anti-RSV F-specific nanobody, ALX-0171. To characterize the …

Comparative therapeutic potential of ALX-0171 and palivizumab against RSV clinical isolate infection of well-differentiated primary pediatric bronchial epithelial cell cultures Open

Lindsay Broadbent, Hong Guo-Parke, Lyndsey J. Ferguson, Andrena Miller, Michael D. Shields , et al. · 2019

Respiratory syncytial virus (RSV) causes severe lower respiratory tract infections in young infants. There are no RSV-specific treatments available. Ablynx has been developing an anti-RSV F-specific Nanobody®, ALX-0171. To characterise the…

Airway Epithelial Derived Cytokines and Chemokines and Their Role in the Immune Response to Respiratory Syncytial Virus Infection Open

Lena Glaser, Patricia Coulter, Michael D. Shields, Olivier Touzelet, Ultan F. Power , et al. · 2019

The airway epithelium is the primary target of respiratory syncytial virus infection. It is an important component of the antiviral immune response. It contributes to the recruitment and activation of innate immune cells from the periphery…

The secretome profiling of a pediatric airway epithelium infected with hRSV identified aberrant apical/basolateral trafficking and novel immune modulating (CXCL6, CXCL16, CSF3) and antiviral (CEACAM1) proteins. Open

Olivier Touzelet, Lindsay Broadbent, Stuart D. Armstrong, Aljabr Waleed, Elaine Cloutman-Green , et al. · 2019

The respiratory epithelium comprises polarized cells at the interface between the environment and airway tissues. Polarized apical and basolateral protein secretions are a feature of airway epithelium homeostasis. Human respiratory syncyti…

The secretome profiling of a pediatric airway epithelium infected with human respiratory syncytial virus (hRSV) identified aberrant apical/basolateral trafficking and novel immune modulating (CXCL6, CXCL16, CSF3) and antiviral (CEACAM1) proteins Open

Olivier Touzelet, Lindsay Broadbent, Stuart D. Armstrong, Waleed Aljabr, Elaine Cloutman-Green , et al. · 2019

Rationale The respiratory epithelium comprises polarized cells at the interface between the environment and airway tissues. Polarized apical and basolateral protein secretions are a feature of airway epithelium homeostasis. Human respirato…

Comparative responses of paediatric airway epithelium to viral and allergen insult Open

Sheerien Manzoor, Lindsay Broadbent, Caoimhe McDermott, Stephen Main, Steve Turner , et al. · 2019

Asthma affects over a million children in the UK. Early age viral infection, allergen sensitization, atopy and genetic predisposition increases the likelihood of developing asthma. Our aim is to understand the consequences of allergen expo…

Influence of gestational and developmental age on human airway epithelial innate immune responses to Respiratory Syncytial Virus (RSV) in early life Open

Helen Groves, Lindsay Broadbent, Hong Guo-Parke, Mike Shields, Ultan F. Power · 2019

Background RSV is a major respiratory pathogen in infants, with preterm and young infants being at increased risk of severe disease. Airway epithelial cell (AEC) innate immune responses represent an important first line of defence. Develop…

Early life innate immune responses to RSV in cystic fibrosis airway epithelium Open

Patricia Coulter, Helen Groves, Lindsay Broadbent, Jonathan Dean Coey, Michael D. Shields , et al. · 2019

Respiratory syncytial virus (RSV) infection is the major cause of severe lower respiratory tract disease in young infants. Evidence suggests that cystic fibrosis (CF) is associated with significant morbidity following RSV infection. We and…

Identification of novel factors associated with severe respiratory syncytial virus disease in infants Open

Hong Guo-Parke, Helen Groves, Lindsay Broadbent, Olivier Touzelet, Isobel Douglas , et al. · 2019

Background Almost all infants are infected with RSV by 2 years. 1–3 % of RSV-infected infants are hospitalised with severe disease. Reasons for susceptibility to severe disease remain obscure. We aimed to identify factors that might explai…

Correction: Characterisation of morphological differences in well-differentiated nasal epithelial cell cultures from preterm and term infants at birth and one-year Open

Helen Groves, Hong Guo-Parke, Lindsay Broadbent, Michael D. Shields, Ultan F. Power · 2019

[This corrects the article DOI: 10.1371/journal.pone.0201328.].

Characterisation of morphological differences in well-differentiated nasal epithelial cell cultures from preterm and term infants at birth and one-year Open

Helen Groves, Hong Guo-Parke, Lindsay Broadbent, Michael D. Shields, Ultan F. Power · 2018

We describe the successful generation of newborn-derived WD-PNEC cultures and their revival from frozen. We also compared the characteristics of WD-PNECs derived from infants born at term with those born prematurely at birth and at one-yea…

The Role of Toll-like receptor 4 in respiratory syncytial virus replication, interferon lambda 1 induction, and chemokine responses Open

Lindsay Broadbent, Jonathon D. Coey, Michael D. Shields, Ultan F. Power · 2018

Respiratory syncytial virus (RSV) infection is the leading cause of severe lower respiratory tract infections (LRTI) in infants worldwide. The immune responses to RSV infection are implicated in RSV pathogenesis but RSV immunopathogenesis …

Characterisation of morphological differences in well-differentiated nasal epithelial cell cultures from preterm and term infants at birth and one-year Open

Helen Groves, Hong Guo-Parke, Lindsay Broadbent, Michael D. Shields, Ultan F. Power · 2018

Background Innate immune responses of airway epithelium are important defences against respiratory pathogens and allergens. Newborn infants are at greater risk of severe respiratory infections compared to older infants. However, very littl…

The RNA binding protein La/SS-B promotes RIG-I-mediated type I and type III IFN responses following Sendai viral infection Open

Rebecca Mahony, Lindsay Broadbent, Jacen S. Maier‐Moore, Ultan F. Power, Caroline A. Jefferies · 2017

Antibody-Induced Internalization of the Human Respiratory Syncytial Virus Fusion Protein Open

Alexander Leemans, Marjorie De Schryver, Winke Van der Gucht, Annick Heykers, Isabel Pintelon , et al. · 2017

Respiratory syncytial virus (RSV) infections remain a major cause of respiratory disease and hospitalizations among infants. Infection recurs frequently and establishes a weak and short-lived immunity. To date, RSV immunoprophylaxis and va…

Induction and Antagonism of Antiviral Responses in Respiratory Syncytial Virus-Infected Pediatric Airway Epithelium Open

Rémi Villenave, Lindsay Broadbent, Isobel Douglas, Jeremy Lyons, Peter Coyle , et al. · 2015

Airway epithelium is the primary target of many respiratory viruses. However, virus induction and antagonism of host responses by human airway epithelium remains poorly understood. To address this, we developed a model of respiratory syncy…

Respiratory syncytial virus, an ongoing medical dilemma: an expert commentary on respiratory syncytial virus prophylactic and therapeutic pharmaceuticals currently in clinical trials Open

Lindsay Broadbent, Helen Groves, Michael D. Shields, Ultan F. Power · 2015

As the most important viral cause of severe respiratory disease in infants and increasing recognition as important in the elderly and immunocompromised, respiratory syncytial virus ( RSV ) is responsible for a massive health burden worldwi…

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