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View article: Protocol for identification of sgRNA mutants using high-throughput screening technique and multiplex genome editing
Protocol for identification of sgRNA mutants using high-throughput screening technique and multiplex genome editing Open
In the CRISPR-Cas9 system, tandem expression of multiple identical single-guide RNAs (sgRNAs) often triggers homologous sequences loss, which affects multiplex genome editing efficiencies. Here, we present a protocol for high-throughput sc…