Lisa M. Larkin
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View article: Impact of Human Recombinant Irisin on Tissue-Engineered Skeletal Muscle Structure and Function
Impact of Human Recombinant Irisin on Tissue-Engineered Skeletal Muscle Structure and Function Open
Tissue engineering of exogenous skeletal muscle units (SMUs) through isolation of muscle satellite cells from muscle biopsies is a potential treatment method for acute volumetric muscle loss (VML). A current issue with this treatment proce…
View article: Engineered Tissue Graft for Repair of Injured Infraspinatus Rotator Cuff Tendon
Engineered Tissue Graft for Repair of Injured Infraspinatus Rotator Cuff Tendon Open
Rotator cuff tears constitute a vast majority of shoulder-related injuries, occurring in a wide population range and increasing in incidence with age. Current treatments for full thickness tears use suture to secure the ruptured tendon bac…
View article: Impact of Cell Seeding Density and Cell Confluence on Human Tissue Engineered Skeletal Muscle
Impact of Cell Seeding Density and Cell Confluence on Human Tissue Engineered Skeletal Muscle Open
Tissue engineering methodologies have the potential to treat volumetric muscle loss via the growth of exogenous skeletal muscle grafts from small autogenous muscle biopsies. A significant obstacle preventing the widespread use of engineere…
View article: Impact of Human Epidermal Growth Factor on Tissue-Engineered Skeletal Muscle Structure and Function
Impact of Human Epidermal Growth Factor on Tissue-Engineered Skeletal Muscle Structure and Function Open
Skeletal muscle tissue engineering technologies have the potential to treat volumetric muscle loss (VML) by growing exogenous muscle tissue. However, there has been limited success in engineering human cell-sourced skeletal muscle with str…
View article: A tissue engineering approach for repairing craniofacial volumetric muscle loss in a sheep following a 2, 4, and 6-month recovery
A tissue engineering approach for repairing craniofacial volumetric muscle loss in a sheep following a 2, 4, and 6-month recovery Open
Volumetric muscle loss (VML) is the loss of skeletal muscle that results in significant and persistent impairment of function. The unique characteristics of craniofacial muscle compared trunk and limb skeletal muscle, including differences…
View article: Repairing Volumetric Muscle Loss in the Ovine Peroneus Tertius Following a 3-Month Recovery
Repairing Volumetric Muscle Loss in the Ovine Peroneus Tertius Following a 3-Month Recovery Open
Much effort has been made to fabricate engineered tissues on a scale that is clinically relevant to humans; however, scale-up remains one of the most significant technological challenges of tissue engineering to date. To address this limit…
View article: A Comparison of Ovine Facial and Limb Muscle as a Primary Cell Source for Engineered Skeletal Muscle
A Comparison of Ovine Facial and Limb Muscle as a Primary Cell Source for Engineered Skeletal Muscle Open
Volumetric muscle loss (VML) contributes to the number of soft tissue injuries that necessitate reconstructive surgery, but treatment options are often limited by tissue availability and donor site morbidity. To combat these issues, our la…
View article: A Transgenic tdTomato Rat for Cell Migration and Tissue Engineering Applications
A Transgenic tdTomato Rat for Cell Migration and Tissue Engineering Applications Open
The growing deficit in suitable tissues for patients awaiting organ transplants demonstrates the clinical need for engineered tissues as alternative graft sources. Demonstrating safety and efficacy by tracking the migration and fate of imp…
View article: Effects of Dexamethasone Dose and Timing on Tissue-Engineered Skeletal Muscle Units
Effects of Dexamethasone Dose and Timing on Tissue-Engineered Skeletal Muscle Units Open
Our lab showed that administration of dexamethasone (DEX) stimulated myogenesis and resulted in advanced structure in our engineered skeletal muscle units (SMU). While administration of 25 nM DEX resulted in the most advanced structure, 10…
View article: Label-Free, High-Throughput Purification of Satellite Cells Using Microfluidic Inertial Separation
Label-Free, High-Throughput Purification of Satellite Cells Using Microfluidic Inertial Separation Open
Skeletal muscle satellite cells have tremendous therapeutic potential in cell therapy or skeletal muscle tissue engineering. Obtaining a sufficiently pure satellite cell population, however, presents a significant challenge. We hypothesize…
View article: Quantitative, Label-Free Evaluation of Tissue-Engineered Skeletal Muscle Through Multiphoton Microscopy
Quantitative, Label-Free Evaluation of Tissue-Engineered Skeletal Muscle Through Multiphoton Microscopy Open
The lack of tools for assessing engineered tissue viability and function in a noninvasive manner is a major regulatory and translational challenge facing tissue engineers. Label-free, nonlinear optical molecular imaging (OMI) has utilized …
View article: Role of nerve–muscle interactions and reactive oxygen species in regulation of muscle proteostasis with ageing
Role of nerve–muscle interactions and reactive oxygen species in regulation of muscle proteostasis with ageing Open
Skeletal muscle ageing is characterised by atrophy, a deficit in specific force generation, increased susceptibility to injury, and incomplete recovery after severe damage. The hypothesis that increased generation of reactive oxygen specie…
View article: Tissue‐engineered tendon constructs for rotator cuff repair in sheep
Tissue‐engineered tendon constructs for rotator cuff repair in sheep Open
Current rotator cuff repair commonly involves the use of single or double row suture techniques, and despite successful outcomes, failure rates continue to range from 20 to 95%. Failure to regenerate native biomechanical properties at the …
View article: Tissue-Engineered Tendon for Enthesis Regeneration in a Rat Rotator Cuff Model
Tissue-Engineered Tendon for Enthesis Regeneration in a Rat Rotator Cuff Model Open
Healing of rotator cuff (RC) injuries with current suture or augmented scaffold techniques fails to regenerate the enthesis and instead forms a weaker fibrovascular scar that is prone to subsequent failure. Regeneration of the enthesis is …
View article: <i>In vivo</i> structural and cellular remodeling of engineered bone–ligament–bone constructs used for anterior cruciate ligament reconstruction in sheep
<i>In vivo</i> structural and cellular remodeling of engineered bone–ligament–bone constructs used for anterior cruciate ligament reconstruction in sheep Open
Anterior cruciate ligament (ACL) ruptures rank among the most prevalent and costly sports-related injuries. Current tendon grafts used for ACL reconstruction are limited by suboptimal biomechanical properties. We have addressed these issue…
View article: Fresh and Frozen Tissue-Engineered Three-Dimensional Bone–Ligament–Bone Constructs for Sheep Anterior Cruciate Ligament Repair Following a 2-Year Implantation
Fresh and Frozen Tissue-Engineered Three-Dimensional Bone–Ligament–Bone Constructs for Sheep Anterior Cruciate Ligament Repair Following a 2-Year Implantation Open
Injuries to the anterior cruciate ligament (ACL) often require surgical reconstruction utilizing tendon grafts to restore knee function and stability. Some current graft options for ACL repair are associated with poor long-term outcomes. O…
View article: Development of a Regenerative Peripheral Nerve Interface for Control of a Neuroprosthetic Limb
Development of a Regenerative Peripheral Nerve Interface for Control of a Neuroprosthetic Limb Open
Background . The purpose of this experiment was to develop a peripheral nerve interface using cultured myoblasts within a scaffold to provide a biologically stable interface while providing signal amplification for neuroprosthetic control …
View article: Growth Factors for Skeletal Muscle Tissue Engineering
Growth Factors for Skeletal Muscle Tissue Engineering Open
Tissue-engineered skeletal muscle holds promise as a source of graft tissue for repair of volumetric muscle loss and as a model system for pharmaceutical testing. To reach this potential, engineered tissues must advance past the neonatal p…