Lisheng Kong
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View article: Effects of Explant Source and Orientation on Secondary Somatic Embryogenesis in Hevea brasiliensis
Effects of Explant Source and Orientation on Secondary Somatic Embryogenesis in Hevea brasiliensis Open
Propagation of rubber tree (Hevea brasiliensis) via secondary somatic embryogenesis (SSEis) is a reliable method. However, its efficiency is relatively low. The aim of this study was to understand more about the factors related to SSEis in…
View article: Chemical Composition and Antimicrobial Activities of the Essential Oil Extracted from the Leaves of <i>Phrynium tonkinense Gagnep</i>
Chemical Composition and Antimicrobial Activities of the Essential Oil Extracted from the Leaves of <i>Phrynium tonkinense Gagnep</i> Open
Objective: The genus Phrynium has medicinal value and is effective in relieving sore throat and mouth ulcers. However, the extraction and chemical analysis of essential oils from Phrynium tonkinense Gagnep, have not yet been reported. The …
View article: Long-Term Successional Subculture Dynamics and Their Effects on the Proliferation Efficiency, Embryogenic Potential, and Genetic Stability of Embryogenic Tissues in Larix principis-rupprechtii Mayr
Long-Term Successional Subculture Dynamics and Their Effects on the Proliferation Efficiency, Embryogenic Potential, and Genetic Stability of Embryogenic Tissues in Larix principis-rupprechtii Mayr Open
Larix principis-rupprechtii Mayr, a coniferous species indigenous to Northern China, possesses significant ecological and economic value. Somatic embryogenesis offers a pathway with significant potential for large-scale propagation, long-t…
View article: Effects of plant growth regulators and sucrose on proliferation and quality of embryogenic tissue in Picea pungens
Effects of plant growth regulators and sucrose on proliferation and quality of embryogenic tissue in Picea pungens Open
View article: Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development
Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development Open
View article: Additional file 1 of Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development
Additional file 1 of Analysis of polycomb repressive complex 2 (PRC2) subunits in Picea abies with a focus on embryo development Open
Supplementary Material 1
View article: Involvement of 5mC DNA demethylation <i>via</i> 5-aza-2'-deoxycytidine in regulating gene expression during early somatic embryo development in white spruce (<i>Picea glauca)</i>
Involvement of 5mC DNA demethylation <i>via</i> 5-aza-2'-deoxycytidine in regulating gene expression during early somatic embryo development in white spruce (<i>Picea glauca)</i> Open
DNA methylation plays a crucial role in the development of somatic embryos (SEs) through the regulation of gene expression. To examine the impact of DNA methylation on gene expression during early SE development in Picea glauca, the…
View article: Integrated Lipidomic and Transcriptomic Analysis Reveals Phospholipid Changes in Somatic Embryos of Picea asperata in Response to Partial Desiccation
Integrated Lipidomic and Transcriptomic Analysis Reveals Phospholipid Changes in Somatic Embryos of Picea asperata in Response to Partial Desiccation Open
Partial desiccation treatment (PDT) is an effective technology for promoting the germination and conversion of conifer somatic embryos (SEs). PDT, as a drought stress, induces intensive physiological responses in phospholipid metabolism, w…
View article: Variation, coordination, and trade-offs between needle structures and photosynthetic-related traits across five Picea species: consequences on plant growth
Variation, coordination, and trade-offs between needle structures and photosynthetic-related traits across five Picea species: consequences on plant growth Open
Background Picea species are distributed and planted world-wide due to their great ecological and economic values. It has been reported that Picea species vary widely in growth traits in a given environment, which reflects genetic and phen…
View article: Effects of Medium Supplements on Somatic Embryo Maturation and DNA Methylation in Pseudotsuga gaussenii Flous, a Species under Protection
Effects of Medium Supplements on Somatic Embryo Maturation and DNA Methylation in Pseudotsuga gaussenii Flous, a Species under Protection Open
Pseudotsuga gaussenii is a forest species under protection in China. Propagation via somatic embryogenesis is efficient and needed for recovering this species. In this research, effects of medium supplements, i.e., methylglyoxal-bis (guany…
View article: Cryo-Treatment Enhances the Embryogenicity of Mature Somatic Embryos via the lncRNA–miRNA–mRNA Network in White Spruce
Cryo-Treatment Enhances the Embryogenicity of Mature Somatic Embryos via the lncRNA–miRNA–mRNA Network in White Spruce Open
In conifers, somatic embryogenesis is uniquely initiated from immature embryos in a narrow time window, which is considerably hindered by the difficulty to induce embryogenic tissue (ET) from other tissues, including mature somatic embryos…
View article: Transcriptomic and physiological analysis identifies a gene network module highly associated with brassinosteroid regulation in hybrid sweetgum tissues differing in the capability of somatic embryogenesis
Transcriptomic and physiological analysis identifies a gene network module highly associated with brassinosteroid regulation in hybrid sweetgum tissues differing in the capability of somatic embryogenesis Open
Somatic embryogenesis is a preferred method for large-scale production of forest trees due to its high propagation efficiency. In this study, hybrid sweetgum leaves with phase changes from mature to embryogenic state were selected as exper…
View article: Global Transcriptome and Coexpression Network Analyses Reveal New Insights Into Somatic Embryogenesis in Hybrid Sweetgum (Liquidambar styraciflua × Liquidambar formosana)
Global Transcriptome and Coexpression Network Analyses Reveal New Insights Into Somatic Embryogenesis in Hybrid Sweetgum (Liquidambar styraciflua × Liquidambar formosana) Open
Somatic embryogenesis (SE) is a process of somatic cells that dedifferentiate to totipotent embryonic stem cells and generate embryos in vitro . Despite recent scientific headway in deciphering the difficulties of somatic embryogenesis, th…
View article: Efficient Multi-Sites Genome Editing and Plant Regeneration via Somatic Embryogenesis in Picea glauca
Efficient Multi-Sites Genome Editing and Plant Regeneration via Somatic Embryogenesis in Picea glauca Open
Conifers are the world's major source of timber and pulpwood and have great economic and ecological value. Currently, little research on the application of CRISPR/Cas9, the commonly used genome-editing tool in angiosperms, has been reporte…
View article: Plant Regeneration via Somatic Embryogenesis in Larix principis-rupprechtii Mayr
Plant Regeneration via Somatic Embryogenesis in Larix principis-rupprechtii Mayr Open
Prince Rupprecht’s larch (Larix principis-rupprechtii Mayr) is a native conifer in North China with great economic and ecological values. Somatic embryogenesis (SE) is a powerful tool for the mass clonal propagation in plants. In this stud…
View article: Transcriptomic, Metabolomic, and Physiological Analyses Reveal That the Culture Temperatures Modulate the Cryotolerance and Embryogenicity of Developing Somatic Embryos in Picea glauca
Transcriptomic, Metabolomic, and Physiological Analyses Reveal That the Culture Temperatures Modulate the Cryotolerance and Embryogenicity of Developing Somatic Embryos in Picea glauca Open
Cryopreservation is one of the key technologies for the mass propagation of conifers via somatic embryogenesis. Cryotolerance and embryogenecity of conifer somatic embryos (SEs) could be affected by different temperature treatments, for wh…
View article: Growth and Physiological Responses of Norway Spruce (Picea abies (L.) H. Karst) Supplemented with Monochromatic Red, Blue and Far-Red Light
Growth and Physiological Responses of Norway Spruce (Picea abies (L.) H. Karst) Supplemented with Monochromatic Red, Blue and Far-Red Light Open
Monochromatic red light (R) supplementation is more efficient than blue light (B) in promoting Norway spruce (Picea abies (L.) H. Karst) growth. Transcriptome analysis has revealed that R and B may regulate stem growth by regulating phytoh…
View article: Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey
Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey Open
View article: Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei
Potential function of CbuSPL and gene encoding its interacting protein during flowering in Catalpa bungei Open
Background “Bairihua”, a variety of the Catalpa bungei , has a large amount of flowers and a long flowering period which make it an excellent material for flowering researches in trees. SPL is one of the hub genes that regulate both flower…
View article: Transcriptomics and Proteomics Reveal the Cellulose and Pectin Metabolic Processes in the Tension Wood (Non-G-Layer) of Catalpa bungei
Transcriptomics and Proteomics Reveal the Cellulose and Pectin Metabolic Processes in the Tension Wood (Non-G-Layer) of Catalpa bungei Open
Catalpa bungei is an economically important tree with high-quality wood and highly valuable to the study of wood formation. In this work, the xylem microstructure of C. bungei tension wood (TW) was observed, and we performed transcriptomic…
View article: Additional file 20 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey
Additional file 20 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey Open
Additional file 20: Table S11. Summary of AS events from DEGs in NF.
View article: Additional file 19 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey
Additional file 19 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey Open
Additional file 19: Table S10. Summary of AS events from DEGs in EF.
View article: Additional file 21 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey
Additional file 21 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey Open
Additional file 21: Table S12. Summary of primers used in this study.
View article: Additional file 14 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey
Additional file 14 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey Open
Additional file 14: Table S5. Top 20 KEGG pathways significantly enriched with DEGs in three different stages between EF and NF.
View article: Additional file 10 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey
Additional file 10 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey Open
Additional file 10: Table S1. Summary of Illumina short reads.
View article: Additional file 18 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey
Additional file 18 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey Open
Additional file 18: Table S9. Summary of identification of splicing junctions using NGS and SMRT data.
View article: Additional file 12 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey
Additional file 12 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey Open
Additional file 12: Table S3. Top 20 KEGG pathways significantly enriched with DEGs during floral transition in EF.
View article: Additional file 13 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey
Additional file 13 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey Open
Additional file 13: Table S4. All predicted transcription factors in EF during floral transition.
View article: Additional file 15 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey
Additional file 15 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey Open
Additional file 15: Table S6. Summary of all genes from the MEdarkturquoise module.
View article: Additional file 17 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey
Additional file 17 of Multi-omics sequencing provides insight into floral transition in Catalpa bungei. C.A. Mey Open
Additional file 17: Table S8. The correlation coefficients of expression of mRNAs between the EF and NF during three developmental periods. r is the correlation coefficient of expression of the mRNAs.