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View article: A rapid and efficient red-light-activated Cre recombinase system for genome engineering in mammalian cells and transgenic mice
A rapid and efficient red-light-activated Cre recombinase system for genome engineering in mammalian cells and transgenic mice Open
The Cre-loxP recombination system enables precise genome engineering; however, existing photoactivatable Cre tools suffer from several limitations, including low DNA recombination efficiency, background activation, slow activation kinetics…
Drp1 Proteins Released from Hydrolysis-Driven Scaffold Disassembly Trigger Nucleotide-Dependent Membrane Remodeling to Promote Scission Open
Dynamin-related protein (Drp1) drives mitochondrial fission, dysregulation of which leads to neurodegenerative, metabolic, and apoptotic disorders. The precise mechanism of fission completion is unclear. One prevailing model is based on GT…
Dynamin-like Proteins Combine Mechano-constriction and Membrane Remodeling to Enable Two-Step Mitochondrial Fission via a “Snap-through” Instability Open
Mitochondrial fission is controlled by dynamin-like proteins, the dysregulation of which is correlated with diverse diseases. Fission dynamin-like proteins are GTP hydrolysis-driven mechanoenzymes that self-oligomerize into helical structu…
Drp1 Proteins Released from Hydrolysis-driven Scaffold Disassembly Trigger Nucleotide-dependent Membrane Remodeling to Promote Scission Open
Dynamin-related protein (Drp1) drives mitochondrial fission, dysregulation of which leads to neurodegenerative, metabolic, and apoptotic disorders. The precise mechanism of fission completion is unclear. One prevailing model is based on GT…
View article: A rapid and efficient red-light-activated Cre recombinase system for genome engineering in mammalian cells and transgenic mice
A rapid and efficient red-light-activated Cre recombinase system for genome engineering in mammalian cells and transgenic mice Open
The Cre-loxP recombination system enables precise genome engineering; however, existing photoactivatable Cre tools suffer from several limitations, including low DNA recombination efficiency, background activation, slow activation kinetics…
Functional optic tract rewiring via subtype- and target-specific axonal regeneration and presynaptic activity enhancement Open
Mechanisms underlying functional axonal rewiring after adult mammalian central nervous system (CNS) injuries remain unclear partially due to limited models. Here we develop a mouse intracranial pre–olivary pretectal nucleus (OPN) optic tra…
Optogenetic control of mitochondrial aggregation and function Open
The balance of mitochondrial fission and fusion plays an important role in maintaining the stability of cellular homeostasis. Abnormal mitochondrial fission and fragmentation have been shown to be associated with oxidative stress, which ca…
View article: Astragaloside IV alleviates GDM via regulating gut microbiota and gut microbiota metabolomic
Astragaloside IV alleviates GDM via regulating gut microbiota and gut microbiota metabolomic Open
Background Gestational diabetes mellitus (GDM), a severe pregnancy disorder, is a temporary form of diabetes that occurs during gestation. Astragaloside IV (AS IV), a natural and effective composition of Astragalus membranaceus , shows pha…
Clickable, Thermally Responsive Hydrogels Enabled by Recombinant Spider Silk Protein and Spy Chemistry for Sustained Neurotrophin Delivery Open
The ability to deliver protein therapeutics in a minimally invasive, safe, and sustained manner, without resorting to viral delivery systems, will be crucial for treating a wide range of chronic injuries and diseases. Among these challenge…
View article: Prenatal High‐Sucrose Diet Induced Vascular Dysfunction of Renal Interlobar Arteries in the Offspring via PPARγ‐RXRg‐ROS/Akt Signaling
Prenatal High‐Sucrose Diet Induced Vascular Dysfunction of Renal Interlobar Arteries in the Offspring via PPARγ‐RXRg‐ROS/Akt Signaling Open
Scope Prenatal nutrition imbalance correlates with developmental origin of cardiovascular diseases; however whether maternal high‐sucrose diet (HS) during pregnancy causes vascular damage in renal interlobar arteries (RIA) from offspring s…
Selective activation of photoactivatable fluorescent protein based on binary holography Open
The ability to deliver laser doses to different target locations with high spatial and temporal resolution has been a long-sought goal in photo-stimulation and optogenetics research via, for example, photoactivatable proteins. These light-…
View article: Using an ER-specific optogenetic mechanostimulator to understand the mechanosensitivity of the endoplasmic reticulum
Using an ER-specific optogenetic mechanostimulator to understand the mechanosensitivity of the endoplasmic reticulum Open
The ability of cells to perceive and respond to mechanical cues is essential for numerous biological activities. Emerging evidence indicates important contributions of organelles to cellular mechanosensitivity and mechanotransduction. Howe…
View article: Force-induced tail-autotomy mitochondrial fission and biogenesis of matrix-excluded mitochondrial-derived vesicles for quality control
Force-induced tail-autotomy mitochondrial fission and biogenesis of matrix-excluded mitochondrial-derived vesicles for quality control Open
Mitochondria constantly fuse and divide for mitochondrial inheritance and functions. Here, we identified a distinct type of naturally occurring fission, tail-autotomy fission, wherein a tail-like thin tubule protrudes from the mitochondria…
Compact Morpho‐Molecular Microscopy for Live‐cell Imaging and Material Characterization Open
The Compact Morpho‐Molecular Microscopy (CM 3 ) is proposed and demonstrated for multi‐functional imaging and characterization of living cells and material structures by simultaneously offering quantitative phase imaging (QPI), dispersion …
View article: Mechanical force induces DRP1-dependent asymmetrical mitochondrial fission for quality control
Mechanical force induces DRP1-dependent asymmetrical mitochondrial fission for quality control Open
Mitochondria are membrane-bound organelles that perform diverse critical biological functions. They undergo constant fission and fusion, which are important for mitochondrial inheritance, functions, and quality control. While tremendous ef…
xSCYTE: Express Single-frame Cytometer through Tomographic Phase Open
Rapid, comprehensive, and accurate cell phenotyping without compromising viability, is crucial to many important biomedical applications, including stem-cell therapy, drug screening, and liquid biopsy. Typical image cytometry methods acqui…
Optogenetic activation of intracellular signaling based on light-inducible protein-protein homo-interactions Open
Dynamic protein-protein interactions are essential for proper cell functioning. Homo-interaction events-physical interactions between the same type of proteins-represent a pivotal subset of protein-protein interactions that are widely expl…
Optical activation of TrkB receptors Open
Brain-derived neurotrophic factor (BDNF), via activation of tropomyosin receptor kinase B (TrkB), plays a critical role in neuronal proliferation, differentiation, survival, and death. Dysregulation of TrkB signaling is implicated in neuro…
Membrane curvature underlies actin reorganization in response to nanoscale surface topography Open
Significance Despite a large body of work, little is known about the origin or underlying mechanisms of how cells interact with surface topographic patterns, which has resulted in a primarily phenomenological approach in studying cell–nano…
Optical Activation of TrkA Signaling Open
Nerve growth factor/tropomyosin receptor kinase A (NGF/TrkA) signaling plays a key role in neuronal development, function, survival, and growth. The pathway is implicated in neurodegenerative disorders including Alzheimer's disease, chroni…
Optical activation of TrkA signaling Open
Nerve growth factor/tropomyosin receptor kinase A (NGF/TrkA) signaling plays a key role in neuronal development, function, survival, and growth. The pathway is implicated in neurodegenerative disorders including Alzheimer’s disease, chroni…
Understanding CRY2 interactions for optical control of intracellular signaling Open
Arabidopsis cryptochrome 2 (CRY2) can simultaneously undergo light-dependent CRY2–CRY2 homo-oligomerization and CRY2–CIB1 hetero-dimerization, both of which have been widely used to optically control intracellular processes. Applications u…
Revealing the Cell–Material Interface with Nanometer Resolution by Focused Ion Beam/Scanning Electron Microscopy Open
The interface between cells and nonbiological surfaces regulates cell attachment, chronic tissue responses, and ultimately the success of medical implants or biosensors. Clinical and laboratory studies show that topological features of the…
Revealing the cell-material interface with nanometer resolution by FIB-SEM Open
The interface between biological cells and non-biological surfaces profoundly influences cellular activities, chronic tissue responses, and ultimately the success of medical implants. Materials in contact with cells can be plastics, metal,…
The Timing of Raf/ERK and AKT Activation in Protecting PC12 Cells against Oxidative Stress Open
Acute brain injuries such as ischemic stroke or traumatic brain injury often cause massive neural death and irreversible brain damage with grave consequences. Previous studies have established that a key participant in the events leading t…