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View article: Synthetic biology-inspired development of live attenuated influenza vaccines
Synthetic biology-inspired development of live attenuated influenza vaccines Open
Live attenuated influenza vaccines (LAIVs) provide robust, cross-protective immunity but have traditionally been developed empirically and are associated with safety concerns. Recent advances in rational design enable precise genetic modif…
View article: Natural Infection of Omicron BA.5.2 in Patients Provides Broad Immune Responses Against SARS-CoV-2
Natural Infection of Omicron BA.5.2 in Patients Provides Broad Immune Responses Against SARS-CoV-2 Open
The implementation of COVID-19 policy and the rapid development of SARS-CoV-2 vaccines in the early pandemic significantly contained numerous outbreaks and reduced the severity and mortality of COVID-19. However, the population immunity in…
View article: Host Serine Proteases and Antiviral Innate Immunity as Potential Therapeutic Targets in Influenza A Virus Infection-Induced COPD Exacerbations
Host Serine Proteases and Antiviral Innate Immunity as Potential Therapeutic Targets in Influenza A Virus Infection-Induced COPD Exacerbations Open
Lung manifestations of chronic obstructive pulmonary disease (COPD) are often exacerbated by influenza A virus infections; however, the underlying mechanisms remain largely unknown, and hence therapeutic options are limited. Using a physio…
View article: Inflammation and epithelial–mesenchymal transition in a <scp>CFTR</scp> ‐depleted human bronchial epithelial cell line revealed by proteomics and human organ‐on‐a‐chip
Inflammation and epithelial–mesenchymal transition in a <span>CFTR</span> ‐depleted human bronchial epithelial cell line revealed by proteomics and human organ‐on‐a‐chip Open
Cystic fibrosis (CF) is a genetic disease caused by mutations in the CF transmembrane conductance regulator ( CFTR ) gene, leading to chronic, unresolved inflammation of the airways due to uncontrolled recruitment of polymorphonuclear leuk…
View article: PROTAR Vaccine 2.0 generates influenza vaccines by degrading multiple viral proteins
PROTAR Vaccine 2.0 generates influenza vaccines by degrading multiple viral proteins Open
Manipulating viral protein stability using the cellular ubiquitin-proteasome system (UPS) represents a promising approach for developing live-attenuated vaccines. The first-generation proteolysis-targeting (PROTAR) vaccine had limitations,…
View article: Cross-species recognition of two porcine coronaviruses to their cellular receptor aminopeptidase N of dogs and seven other species
Cross-species recognition of two porcine coronaviruses to their cellular receptor aminopeptidase N of dogs and seven other species Open
Porcine deltacoronavirus (PDCoV) and transmissible gastroenteritis coronavirus (TGEV), the two causative agents of porcine diarrhea, have been reported to be at risk of cross-species transmission, including to humans. However, the potentia…
View article: A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse Model
A Subunit Vaccine Harboring the Fusion Capsid Proteins of Porcine Circovirus Types 2, 3, and 4 Induces Protective Immune Responses in a Mouse Model Open
Coinfections with porcine circovirus types 2, 3, and 4 (PCV2, PCV3, and PCV4) are increasingly being detected in the swine industry. However, there is no commercially available vaccine which prevents coinfection with PCV2, PCV3, and PCV4. …
View article: Combining Scalable Organ Chip Platform with Deep Learning-Based Imaging Analysis for Cancer Therapeutic Screening
Combining Scalable Organ Chip Platform with Deep Learning-Based Imaging Analysis for Cancer Therapeutic Screening Open
Functional precision oncology represents an emerging approach to genomic approaches by testing treatment options directly on patient-derived models. Current assays, including the use of patient-derived xenograft (PDX) and patient-derived o…
View article: Rapid Prototyping of Thermoplastic Microfluidic 3D Cell Culture Devices by Creating Regional Hydrophilicity Discrepancy (Adv. Sci. 7/2024)
Rapid Prototyping of Thermoplastic Microfluidic 3D Cell Culture Devices by Creating Regional Hydrophilicity Discrepancy (Adv. Sci. 7/2024) Open
Rapid Prototyping Thermoplastic Organ Chips In article number 2304332 by Yu Shrike Zhang, Xin Xie, and co‐workers, an approach to pattern fluid guides inside microchannels is reported, resulting in rapid and consistent hydrogel flow in ful…
View article: DNA origami vaccine (DoriVac) nanoparticles improve both humoral and cellular immune responses to infectious diseases
DNA origami vaccine (DoriVac) nanoparticles improve both humoral and cellular immune responses to infectious diseases Open
Current SARS-CoV-2 vaccines have demonstrated robust induction of neutralizing antibodies and CD4+ T cell activation, however CD8+ responses are variable, and the duration of immunity and protection against variants are limited. Here we re…
View article: Rapid Prototyping of Thermoplastic Microfluidic 3D Cell Culture Devices by Creating Regional Hydrophilicity Discrepancy
Rapid Prototyping of Thermoplastic Microfluidic 3D Cell Culture Devices by Creating Regional Hydrophilicity Discrepancy Open
Microfluidic 3D cell culture devices that enable the recapitulation of key aspects of organ structures and functions in vivo represent a promising preclinical platform to improve translational success during drug discovery. Essential to th…
View article: Infection of Omicron BA.5.2 in patients provides broad immune responses against SARS-CoV-2
Infection of Omicron BA.5.2 in patients provides broad immune responses against SARS-CoV-2 Open
The host immunity induced by wide spread of Omicron variants and its influence on emerging SARS-CoV-2 variants are attracting broad attention. Here we report that the natural infection with Omicron BA.5.2 induce broad cross-reactive immuni…
View article: Infection of Omicron BA.5.2 in patients provides broad immune responses against SARS-CoV-2
Infection of Omicron BA.5.2 in patients provides broad immune responses against SARS-CoV-2 Open
The host immunity induced by wide spread of Omicron variants and its influence on emerging SARS-CoV-2 variants are attracting broad attention. Here we report that the natural infection with Omicron BA.5.2 induce broad cross-reactive immuni…
View article: Infection of Omicron BA.5.2 in patients provides broad immune responses against SARS-CoV-2
Infection of Omicron BA.5.2 in patients provides broad immune responses against SARS-CoV-2 Open
The host immunity induced by wide spread of Omicron variants and its influence on emerging SARS-CoV-2 variants are attracting broad attention. Here we report that the natural infection with Omicron BA.5.2 induce broad cross-reactive immuni…
View article: Infection of Omicron BA.5.2 in patients provides broad immune responses against SARS-CoV-2
Infection of Omicron BA.5.2 in patients provides broad immune responses against SARS-CoV-2 Open
The host immunity induced by wide spread of Omicron variants and its influence on emerging SARS-CoV-2 variants are attracting broad attention. Here we report that the natural infection with Omicron BA.5.2 induce broad cross-reactive immuni…
View article: Rapid Prototyping of Thermoplastic Microfluidic 3D Cell Culture Devices by Creating Regional Hydrophilicity Discrepancy
Rapid Prototyping of Thermoplastic Microfluidic 3D Cell Culture Devices by Creating Regional Hydrophilicity Discrepancy Open
Microfluidic three-dimensional cell culture devices that enable the recapitulation of key aspects of organ structures and functions in vivo represent a promising preclinical platform to improve translational success during drug discovery. …
View article: Advancements in organs-on-chips technology for viral disease and anti-viral research
Advancements in organs-on-chips technology for viral disease and anti-viral research Open
Disease models that can accurately recapitulate human pathophysiology during infection and clinical response to antiviral therapeutics are still lacking, which represents a major barrier in drug development. The emergence of human Organs-o…
View article: Optimization and applications of an in vivo bioluminescence imaging model of influenza A virus infections
Optimization and applications of an in vivo bioluminescence imaging model of influenza A virus infections Open
• The in vivo BLI model of IAV infections can simplify the determination of viral load in living animals. • The in vivo BLI model of IAV infections allow longitudinal measurements of virus infection/spread in living animals. • The in vivo …
View article: PROTAC vaccine: A new way to live attenuated vaccines
PROTAC vaccine: A new way to live attenuated vaccines Open
While the SARS-CoV-2 pandemic is attracting attention from all over the world, influenza remains a major challenge to global health. Despite the use of existing vaccines, influenza still causes 3–5 million severe cases and .3–.65 million d…
View article: 3D Lung Tissue Models for Studies on SARS-CoV-2 Pathophysiology and Therapeutics
3D Lung Tissue Models for Studies on SARS-CoV-2 Pathophysiology and Therapeutics Open
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing the coronavirus disease 2019 (COVID-19), has provoked more than six million deaths worldwide and continues to pose a major threat to global health. Enormous efforts have…
View article: SNTA1-deficient human cardiomyocytes are associated with increased structural components, calcium handling disorder, and shorter field potential duration
SNTA1-deficient human cardiomyocytes are associated with increased structural components, calcium handling disorder, and shorter field potential duration Open
Background α-1-syntrophin (SNTA1), a protein encoded by SNTA1 , is highly expressed in human cardiomyocytes. Mutations in SNTA1 are associated with arrhythmia and cardiomyopathy. Previous research on SNTA1 has been based on nonhuman cardio…
View article: Self-assembling short immunostimulatory duplex RNAs with broad spectrum antiviral activity
Self-assembling short immunostimulatory duplex RNAs with broad spectrum antiviral activity Open
The current COVID-19 pandemic highlights the need for broad-spectrum antiviral therapeutics. Here we describe a new class of self-assembling immunostimulatory short duplex RNAs that potently induce production of type I and type III interfe…
View article: Clinically Relevant Influenza Virus Evolution Reconstituted in a Human Lung Airway-on-a-Chip
Clinically Relevant Influenza Virus Evolution Reconstituted in a Human Lung Airway-on-a-Chip Open
The rapid evolution of viruses, such as influenza viruses and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is challenging the use and development of antivirals and vaccines. Studies of within-host viral evolution can contr…