Lora Pless
YOU?
Author Swipe
View article: Healthcare worker long-sleeved attire contamination: a prospective observational study
Healthcare worker long-sleeved attire contamination: a prospective observational study Open
Objective: Estimate bacterial pathogen contamination of healthcare workers’ (HCW) long-sleeved attire. Design: Prospective observational study. Setting: Tertiary care hospital. Participants: HCWs wearing long-sleeved attire providing direc…
View article: Genomic epidemiology of healthcare-associated respiratory virus infections in Pittsburgh, Pennsylvania, 2018–2020
Genomic epidemiology of healthcare-associated respiratory virus infections in Pittsburgh, Pennsylvania, 2018–2020 Open
Background: Respiratory virus transmission in healthcare settings is not well understood. To investigate the transmission dynamics of common healthcare-associated respiratory virus infections, we performed retrospective whole genome sequen…
View article: ACCIO: An Assembly-Based Tool Enabling Plasmid Detection
ACCIO: An Assembly-Based Tool Enabling Plasmid Detection Open
2. Abstract Plasmids are extrachromosomal mobile genetic elements that often carry genes responsible for antimicrobial resistance. Plasmid epidemiology aims to track the evolution and spread of plasmids, but the field currently faces signi…
View article: Enhanced discrimination of <i>Clostridioides difficile</i> transmission using whole-genome sequencing and <i>in silico</i> multi-locus variable number tandem repeat analysis
Enhanced discrimination of <i>Clostridioides difficile</i> transmission using whole-genome sequencing and <i>in silico</i> multi-locus variable number tandem repeat analysis Open
Whole-genome sequencing (WGS) is used to establish genetic relatedness of bacteria and track outbreaks in healthcare settings. While WGS provides sufficient discriminatory power to make inferences about genetic relatedness and transmission…
View article: Whole Genome Sequencing to Study SARS-CoV-2 Transmission between University Students and the Surrounding Community in Pittsburgh, Pennsylvania, 2020-2021
Whole Genome Sequencing to Study SARS-CoV-2 Transmission between University Students and the Surrounding Community in Pittsburgh, Pennsylvania, 2020-2021 Open
SARS-CoV-2 transmission was investigated between university students and the surrounding community using whole genome sequencing. Fourteen putative transmission clusters were identified. Proximity assessed using ZIP codes showed that clust…
View article: Emergence and Epidemiology of Dominant Variants of Human Metapneumovirus in the United States between 2016 and 2021
Emergence and Epidemiology of Dominant Variants of Human Metapneumovirus in the United States between 2016 and 2021 Open
Human metapneumovirus (HMPV) causes acute respiratory disease worldwide and is the second leading cause of lower respiratory infection and hospitalization in young children in the US. There is no licensed vaccine or therapeutic. HMPV mutat…
View article: Genomic Epidemiology of Healthcare-Associated Respiratory Virus Infections
Genomic Epidemiology of Healthcare-Associated Respiratory Virus Infections Open
Background Respiratory virus transmission in healthcare settings is not well understood. To investigate the transmission dynamics of common healthcare-associated respiratory virus infections, we performed retrospective whole genome sequenc…
View article: Bacteriocin production facilitates nosocomial emergence of vancomycin-resistant Enterococcus faecium
Bacteriocin production facilitates nosocomial emergence of vancomycin-resistant Enterococcus faecium Open
Gastrointestinal colonization by the nosocomial pathogen vancomycin-resistant Enterococcus faecium (VREfm) can lead to bloodstream infections with high mortality rates. Shifts in VREfm lineages found within healthcare settings occur, but r…
View article: 641. Real-Time Whole Genome Sequencing Surveillance of Healthcare Associated SARS-CoV-2, Respiratory Syncytial Virus (RSV) and Influenza
641. Real-Time Whole Genome Sequencing Surveillance of Healthcare Associated SARS-CoV-2, Respiratory Syncytial Virus (RSV) and Influenza Open
Background Seasonal respiratory viruses contribute to substantial healthcare burden, marked by elevated rates of hospitalizations, morbidity and mortality. The transmission dynamics of respiratory viruses in adult acute care hospitals are …
View article: 295. Methods for Cost-efficient Real-time Whole Genome Sequencing Surveillance for Enhanced Detection of Outbreaks in a Hospital Setting
295. Methods for Cost-efficient Real-time Whole Genome Sequencing Surveillance for Enhanced Detection of Outbreaks in a Hospital Setting Open
Background Healthcare associated infections (HAI) are common hospital complications associated with increased length of stay, economic burden, morbidity, and mortality. Traditionally, HAI outbreaks were detected using reactive whole genome…
View article: Transmission of group A Streptococcus in long-term care: An outbreak investigation
Transmission of group A Streptococcus in long-term care: An outbreak investigation Open
We report 2 outbreaks comprising a cluster of 18 group A Streptococcal infections across 2 nursing facilities. Point prevalence surveillance testing identified 8 of 18 cases, and whole genome sequencing validated transmission pathways. Inf…
View article: Genomic Surveillance for Enhanced Healthcare Outbreak Detection and Control
Genomic Surveillance for Enhanced Healthcare Outbreak Detection and Control Open
Background Current methods are insufficient alone for outbreak detection in hospitals. Real-time genomic surveillance using offers the potential to detect otherwise unidentified outbreaks. We initiated and evaluated the Enhanced Detection …
View article: Bacteriocin production facilitates nosocomial emergence of vancomycin-resistant<i>Enterococcus faecium</i>
Bacteriocin production facilitates nosocomial emergence of vancomycin-resistant<i>Enterococcus faecium</i> Open
Vancomycin-resistant Enterococcus faecium (VREfm) is a prevalent healthcare-acquired pathogen. Gastrointestinal colonization can lead to difficult-to-treat bloodstream infections with high mortality rates. Prior studies have investigated V…
View article: Enhanced Discrimination of<i>Clostridioides difficile</i>Transmission Using Whole Genome Sequencing and<i>In-Silico</i>Multi-locus Variable Number Tandem Repeat Analysis
Enhanced Discrimination of<i>Clostridioides difficile</i>Transmission Using Whole Genome Sequencing and<i>In-Silico</i>Multi-locus Variable Number Tandem Repeat Analysis Open
Whole genome sequencing (WGS) is used to establish genetic relatedness of bacteria and track outbreaks in healthcare settings. While WGS provides sufficient discriminatory power to make inferences about genetic relatedness and transmission…
View article: Real-time Whole Genome Sequencing Surveillance as an Effective Outbreak Detection and Mitigation Tool
Real-time Whole Genome Sequencing Surveillance as an Effective Outbreak Detection and Mitigation Tool Open
Background: Detection of outbreaks traditionally relies on passive surveillance, and often misidentify or miss outbreaks. Whole genome sequencing (WGS) surveillance has emerged as a proactive measure, enabling early detection of outbreaks …
View article: Genomic sequencing surveillance of patients colonized with vancomycin-resistant<i>Enterococcus</i>(VRE) improves detection of hospital-associated transmission
Genomic sequencing surveillance of patients colonized with vancomycin-resistant<i>Enterococcus</i>(VRE) improves detection of hospital-associated transmission Open
Background Vancomycin-resistant enterococcal (VRE) infections pose significant challenges in healthcare. Transmission dynamics of VRE are complex, often involving patient colonization and subsequent transmission through various healthcare-…
View article: Methods for cost-efficient, whole genome sequencing surveillance for enhanced detection of outbreaks in a hospital setting
Methods for cost-efficient, whole genome sequencing surveillance for enhanced detection of outbreaks in a hospital setting Open
Introduction Outbreaks of healthcare-associated infections (HAI) result in substantial patient morbidity and mortality; mitigation efforts by infection prevention teams have the potential to curb outbreaks and prevent transmission to addit…
View article: Real-time genomic epidemiologic investigation of a multispecies plasmid-associated hospital outbreak of NDM-5-producing Enterobacterales infections
Real-time genomic epidemiologic investigation of a multispecies plasmid-associated hospital outbreak of NDM-5-producing Enterobacterales infections Open
Our investigation revealed a complex, multispecies outbreak of NDM involving multiple plasmid transfer and bacterial transmission events. We highlight the utility of combining traditional IP&C and prospective genomic methods in identifying…
View article: ICE volume 45 issue 2 Cover and Front matter
ICE volume 45 issue 2 Cover and Front matter Open
An abstract is not available for this content so a preview has been provided. As you have access to this content, a full PDF is available via the ‘Save PDF’ action button.
View article: Prolonged bacterial carriage and hospital transmission detected by whole genome sequencing surveillance
Prolonged bacterial carriage and hospital transmission detected by whole genome sequencing surveillance Open
An abstract is not available for this content. As you have access to this content, full HTML content is provided on this page. A PDF of this content is also available in through the 'Save PDF' action button.
View article: Impact of discontinuation of contact precautions on surveillance- and whole genome sequencing-defined methicillin-resistant <i>Staphylococcus aureus</i> healthcare-associated infections
Impact of discontinuation of contact precautions on surveillance- and whole genome sequencing-defined methicillin-resistant <i>Staphylococcus aureus</i> healthcare-associated infections Open
Objective: Prior studies evaluating the impact of discontinuation of contact precautions (DcCP) on methicillin-resistant Staphylococcus aureus (MRSA) outcomes have characterized all healthcare-associated infections (HAIs) rather than those…
View article: The utility of whole-genome sequencing to inform epidemiologic investigations of SARS-CoV-2 clusters in acute-care hospitals
The utility of whole-genome sequencing to inform epidemiologic investigations of SARS-CoV-2 clusters in acute-care hospitals Open
Objective: To evaluate the utility of selective reactive whole-genome sequencing (WGS) in aiding healthcare-associated cluster investigations. Design: Mixed-methods quality-improvement study. Setting: Thes study was conducted across 8 acut…
View article: 742. Strain Replacement of Vancomycin-Resistant <i>Enterococcus faecium</i> over 6 years at a Large Tertiary Care Center
742. Strain Replacement of Vancomycin-Resistant <i>Enterococcus faecium</i> over 6 years at a Large Tertiary Care Center Open
Background E. faecium is a common hospital-acquired bacteria in the United States, with a high prevalence of vancomycin resistance seen within this species. Hospital-adapted vancomycin-resistant E. faecium (VREfm) lineages, such as ST17 an…
View article: Two Artificial Tears Outbreak-Associated Cases of Extensively Drug-Resistant <i>Pseudomonas aeruginosa</i> Detected Through Whole Genome Sequencing–Based Surveillance
Two Artificial Tears Outbreak-Associated Cases of Extensively Drug-Resistant <i>Pseudomonas aeruginosa</i> Detected Through Whole Genome Sequencing–Based Surveillance Open
We describe 2 cases of extensively drug-resistant Pseudomonas aeruginosa infection caused by a strain of public health concern, as it was recently associated with a nationwide outbreak of contaminated artificial tears. Both cases were dete…
View article: Genomic Epidemiologic Investigation of a Multispecies Hospital Outbreak of NDM-5-Producing Enterobacterales Infections
Genomic Epidemiologic Investigation of a Multispecies Hospital Outbreak of NDM-5-Producing Enterobacterales Infections Open
Background New Delhi metallo-β-lactamase (NDM) represents an emergent mechanism of carbapenem resistance associated with high mortality and limited antimicrobial treatment options. Because the bla NDM resistance gene is often carried on pl…
View article: Environmental contamination of postmortem blood cultures detected by whole-genome sequencing surveillance
Environmental contamination of postmortem blood cultures detected by whole-genome sequencing surveillance Open
An abstract is not available for this content. As you have access to this content, full HTML content is provided on this page. A PDF of this content is also available in through the ‘Save PDF’ action button.
View article: Real-time whole-genome sequencing surveillance for outbreak detection and intervention
Real-time whole-genome sequencing surveillance for outbreak detection and intervention Open
Background: Detecting healthcare-associated transmission and outbreaks often relies on reactive whole-genome sequencing (WGS), which occurs after the suspected transmission has occurred. Additionally, reactive WGS frequently misidentifies …