Explanipedia

P-020 Tracking zinc and calcium distribution in human sperm: insights from nano-elemental imaging on sperm hyperactivation and capacitation Open

Cristina Tufoni, Alessandra Gianoncelli, Simone Sala, Maik Kahnt, Sara Bozzer , et al. · 2025

Study question What is the role and dynamics of zinc and calcium ions redistribution during human sperm capacitation and how does it affect sperm maturation and fertilization? Summary answer Zinc and calcium redistribution during sperm cap…

Endothelial cell supplementation promotes xenograft revascularization during short-term ovarian tissue transplantation Open

Mariagiulia Spazzapan, Silvia Pegoraro, Roman Vuerich, Gabriella Zito, Andrea Balduit , et al. · 2025

Bromine Distribution in Healthy and Diseased Ovarian Tissues Investigated by X‐Ray Fluorescence Microscopy: An Exploratory Study Open

Alessandra Gianoncelli, Anna Flamigni, Sebastian Schöder, Cristina Tufoni, Valentina Bonino , et al. · 2025

Bromine (Br) is found in nature primarily as part of inorganic salts, with a significant presence in seawater, and is also found in soil, contributing to its natural occurrence in human and animal diets. As an element in the human body, br…

High-resolution X-ray phase-contrast tomography of human placenta with different wavefront markers Open

Sara Savatović, Davis Laundon, Fabio De Marco, Mirko Riedel, Jörg U. Hammel , et al. · 2025

The effect of near-infrared Photobiomodulation therapy on the ion content of 50B11 sensory neurons measured through XRF analysis Open

Luisa Zupin, Alessandra Gianoncelli, Fulvio Celsi, Valentina Bonanni, George Kourousias , et al. · 2024

Photobiomodulation therapy (PBMT) is a form of treatment commonly used for routine clinical applications, such as wound healing of the skin and reduction of inflammation. Additionally, PBMT has been explored for its potential in pain relie…

Proangiogenic properties of complement protein C1q can contribute to endometriosis Open

Chiara Agostinis, Miriam Toffoli, Gabriella Zito, Andrea Balduit, Silvia Pegoraro , et al. · 2024

Endometriosis (EM) is defined as the engraftment and proliferation of functional endometrial-like tissue outside the uterine cavity, leading to a chronic inflammatory condition. While the precise etiology of EM remains elusive, recent stud…

Morphological and lipid metabolism alterations in macrophages exposed to model environmental nanoplastics traced by high-resolution synchrotron techniques Open

Federica Zingaro, Alessandra Gianoncelli, Giacomo Ceccone, Giovanni Birarda, Domenico Cassano , et al. · 2023

The release of nanoplastics (NPs) in the environment is a significant health concern for long-term exposed humans. Although their usage has certainly revolutionized several application fields, at nanometer size, NPs can easily interact at …

Upside-Down Preference in the Forskolin-Induced In Vitro Differentiation of 50B11 Sensory Neurons: A Morphological Investigation by Label-Free Non-Linear Microscopy Open

Luisa Zupin, Sotiris Psilodimitrakopoulos, Fulvio Celsi, Lina Papadimitriou, Anthi Ranella , et al. · 2023

In this study, we revealed a peculiar morphological feature of 50B11 nociceptive sensory neurons in in vitro culture related to the forskolin-induced differentiation of these cells growing upside-down on cover glass supports. Multi-photon …

Impact of Sample Preparation Methods on Single-Cell X-ray Microscopy and Light Elemental Analysis Evaluated by Combined Low Energy X-ray Fluorescence, STXM and AFM Open

Lucia Merolle, Lorella Pascolo, Luisa Zupin, Pietro Parisse, Valentina Bonanni , et al. · 2023

Background: Although X-ray fluorescence microscopy is becoming a widely used technique for single-cell analysis, sample preparation for this microscopy remains one of the main challenges in obtaining optimal conditions for the measurements…

Morphological and Chemical Investigation of Ovarian Structures in a Bovine Model by Contrast-Enhanced X-ray Imaging and Microscopy Open

Alessandra Gianoncelli, Gabriela Sena, George Kourousias, Ernesto Pascotto, Paul Tafforeau , et al. · 2023

An improved understanding of an ovary’s structures is highly desirable to support advances in folliculogenesis knowledge and reproductive medicine, with particular attention to fertility preservation options for prepubertal girls with mali…

Detention and mapping of iron and toxic environmental elements in human ovarian endometriosis: A suggested combined role Open

Lorella Pascolo, Maria Pachetti, Anna Camillo, Alice Cernogoraz, Clara Rizzardi , et al. · 2022

Impact of sample preparation methods on single cell X-ray microscopy and light elemental analysis evaluated by combined Low Energy X-ray Fluorescence, STXM and AFM Open

Lucia Merolle, Lorella Pascolo, Luisa Zupin, Pietro Parisse, Valentina Bonanni , et al. · 2022

Background X-ray Fluorescence (XRF) microscopy has become a widespread method to observe the location of elemental components within cell. Although it is becoming a widely used technique in many laboratories and synchrotron facilities, sam…

Strategies and Perspectives for UV Resonance Raman Applicability in Clinical Analyses of Human Sperm RNA Open

Maria Pachetti, Francesco D’Amico, Luisa Zupin, Stefania Luppi, Monica Martinelli , et al. · 2021

Developing a deeper knowledge about the impact of DNA and RNA epigenetic mutations on sperm production and fertilization performance is essential for selecting best quality samples in Assisted Reproductive Technologies (ART). Indeed, sperm…

UV Resonance Raman explores protein structural modification upon fibrillation and ligand interaction Open

Maria Pachetti, Francesco D’Amico, Lorella Pascolo, Stefania Pucciarelli, Alessandro Gessini , et al. · 2021

SARS-CoV-2 Short-Time Infection Produces Relevant Cytopathic Effects in Vero E6 Cell Line Open

Luisa Zupin, Francesco Fontana, Rossella Gratton, Margherita Milani, Libera Clemente , et al. · 2021

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is mainly transmitted through respiratory droplets from positive subjects to susceptible hosts or by direct contact with an infected individual. Our study focuses on the in …

Improving a Rapid Alignment Method of Tomography Projections by a Parallel Approach Open

Francesco Guzzi, George Kourousias, Alessandra Gianoncelli, Lorella Pascolo, Andrea Sorrentino , et al. · 2021

The high resolution of synchrotron cryo-nano tomography can be easily undermined by setup instabilities and sample stage deficiencies such as runout or backlash. At the cost of limiting the sample visibility, especially in the case of bio-…

Soft X-ray Microscopy Techniques for Medical and Biological Imaging at TwinMic—Elettra Open

Alessandra Gianoncelli, Valentina Bonanni, Gianluca Gariani, Francesco Guzzi, Lorella Pascolo , et al. · 2021

Progress in nanotechnology calls for material probing techniques of high sensitivity and resolution. Such techniques are also used for high-impact studies of nanoscale materials in medicine and biology. Soft X-ray microscopy has been succe…

Code and data concerning a fast tomography alignment algorithm Open

Francesco Guzzi, George Kourousias, Alessandra Gianoncelli, Lorella Pascolo, Andrea Sorrentino , et al. · 2021

Code and data for a publication on a fast tomography alignment algorithm. Raw data have been kindly provided by Prof. Dr. Francesco Brun (University of Trieste, Elettra Sincrotrone Trieste)

Scanning Probe Microscopies: Imaging and Biomechanics in Reproductive Medicine Research Open

Laura Andolfi, Alice Battistella, Michele Zanetti, Marco Lazzarino, Lorella Pascolo , et al. · 2021

Basic and translational research in reproductive medicine can provide new insights with the application of scanning probe microscopies, such as atomic force microscopy (AFM) and scanning near-field optical microscopy (SNOM). These microsco…

Blue photobiomodulation LED therapy impacts SARS‐CoV‐2 by limiting its replication in Vero cells Open

Luisa Zupin, Rossella Gratton, Francesco Fontana, Libera Clemente, Lorella Pascolo , et al. · 2021

The study of any intervention able to counteract SARS‐CoV‐2 pandemic is considerably envisaged. It was previously shown, in in vitro models of infections, that the LED blue light is able to decrease the viral load of HSV‐1 and ZIKV. In our…

FTIR Spectroscopy to Reveal Lipid and Protein Changes Induced on Sperm by Capacitation: Bases for an Improvement of Sample Selection in ART Open

Maria Pachetti, Luisa Zupin, Irene Venturin, Elisa Mitri, Rita Boscolo , et al. · 2020

Although being a crucial step for Assisted Reproduction Technologies (ART) success, to date sperm selection is based only on morphology, motility and concentration characteristics. Considering the many possible alterations, there is a grea…

Renin Angiotensin System, COVID-19 and Male Fertility: Any Risk for Conceiving? Open

Lorella Pascolo, Gabriella Zito, Luisa Zupin, Stefania Luppi, Elena Giolo , et al. · 2020

The current knowledge concerning the connection between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the renin–angiotensin system (RAS) system in the male reproductive apparatus is still limited, so dedicated studies ar…

SARS-CoV-2 and the next generations: which impact on reproductive tissues? Open

Luisa Zupin, Lorella Pascolo, Gabriella Zito, Giuseppe Ricci, Sérgio Crovella · 2020

Investigation of genomic DNA methylation by ultraviolet resonant Raman spectroscopy Open

Francesco D’Amico, Paolo Zucchiatti, Katia Latella, Maria Pachetti, Alessandro Gessini , et al. · 2020

Cytosine plays a preeminent role in DNA methylation, an epigenetic mechanism that regulates gene expression, the misregulation of which can lead to severe diseases. Several methods are nowadays employed for assessing the global DNA methyla…

TMPRSS2 and ACE2 Coexpression in SARS-CoV-2 Salivary Glands Infection Open

Lorella Pascolo, Luisa Zupin, M. Melato, Paola Maura Tricarico, Sérgio Crovella · 2020

Xu et al. (2020) suggest that salivary glands are a potential \ntarget of severe acute respiratory syndrome–coronavirus 2 \n(SARS-CoV-2) infection, since the expression of angiotensin \nI–converting enzyme 2 (ACE2), the receptor needed for…

Hard and soft X-ray imaging to resolve human ovarian cortical structures Open

Lorella Pascolo, Gabriela Sena, Alessandra Gianoncelli, Alice Cernogoraz, Geroge Kourousias , et al. · 2019

Laboratory and synchrotron X-ray tomography are powerful tools for non-invasive studies of biological samples at micrometric resolution. In particular, the development of phase contrast imaging is enabling the visualization of sample detai…

Cover Image Open

Iria M. Rio‐Echevarria, Jessica Ponti, Alessia Bogni, Douglas Gilliland, Matteo Altissimo , et al. · 2019

The cover image is based on the Research Article XRF mapping and TEM analysis of coated and uncoated silica nanoparticles in A549 cells and human monocytes, by Iria M. Rio-Echevarria et al., https://doi.org/10.1002/xrs.2999. Acknowledgment…

Gadolinium tissue deposition in the periodontal ligament of mice with reduced renal function exposed to Gd-based contrast agents Open

Riccarda Delfino, Matteo Biasotto, Riccardo Candido, Matteo Altissimo, Marco Stebel , et al. · 2018

Nano-imaging of environmental dust in human lung tissue by soft and hard X-ray fluorescence microscopy Open

Alessandra Gianoncelli, Clara Rizzardi, Damien Salomon, Vincenzo Canzonieri, Lorella Pascolo · 2018

Light element distribution in fresh and frozen–thawed human ovarian tissues: a preliminary study Open

Lorella Pascolo, Irene Venturin, Alessandra Gianoncelli, Roberta Bortul, Gabriella Zito , et al. · 2018

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