Lorena Alamillo
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View article: Longevity of cardiac and skeletal muscle proteins is dependent on tissue and subcellular compartmentation patterns
Longevity of cardiac and skeletal muscle proteins is dependent on tissue and subcellular compartmentation patterns Open
Myocytes are exceptionally long-lived cells that must maintain proteome integrity over decades while adjusting for changes in functional output and metabolic demand. We used in vivo stable isotope labeling combined with mass spectrometry p…
View article: Deuterium labeling enables proteome-wide turnover kinetics analysis in cell culture
Deuterium labeling enables proteome-wide turnover kinetics analysis in cell culture Open
Protein turnover is a critical component of gene expression regulation and cellular homeostasis, yet methods for measuring turnover rates that are scalable and applicable to different models are still needed. We introduce an improved D2O (…
View article: Identifying and controlling inactive and active conformations of a serine protease
Identifying and controlling inactive and active conformations of a serine protease Open
Serine proteases have been proposed to dynamically sample inactive and active conformations, but direct evidence at atomic resolution has remained elusive. Using nuclear magnetic resonance (NMR), we identified a single residue, D164, in ex…
View article: Deuterium labeling enables proteome wide turnover kinetics analysis in cell culture
Deuterium labeling enables proteome wide turnover kinetics analysis in cell culture Open
Summary The half-life of proteins is tightly regulated and underlies many cellular processes. It remains unclear the extent to which proteins are dynamically synthesized and degraded in different cell types and cell states. We introduce an…
View article: Protein Turnover Dynamics Analysis With Subcellular Spatial Resolution
Protein Turnover Dynamics Analysis With Subcellular Spatial Resolution Open
Protein synthesis and degradation (i.e., turnover) forms an important part of protein homeostasis and has been implicated in many age-associated diseases. Different cellular locations, such as organelles and membraneless compartments, ofte…