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View article: Linking extreme light availability to cellular function in algae-dominated communities on the Greenland Ice Sheet
Linking extreme light availability to cellular function in algae-dominated communities on the Greenland Ice Sheet Open
Glacier ice algae of the streptophyte genus Ancylonema bloom on glaciers globally, including the Greenland Ice Sheet. These algae survive under extreme high light conditions in the summer, as well as under very low light or total darkness …
View article: Leaf litter material limits the elimination of organic micropollutants in redox-differentiated lab-scale bank filtration columns
Leaf litter material limits the elimination of organic micropollutants in redox-differentiated lab-scale bank filtration columns Open
Organic micropollutants (OMP) in surface waters can leak into groundwater via bank filtration impacting the quality of drinking water produced from this groundwater. OMP concentrations are often greatly reduced during the passage, however,…
View article: Isolation of Dehalococcoides mccartyi strain (NIT-OBY) and identification of a reductive dehalogenase dechlorinating cis-1,3-dichloropropene but not trans-1,3-dichloropropene to non-toxic propene
Isolation of Dehalococcoides mccartyi strain (NIT-OBY) and identification of a reductive dehalogenase dechlorinating cis-1,3-dichloropropene but not trans-1,3-dichloropropene to non-toxic propene Open
The popular soil fumigant 1,3-dichloropropene (1,3-D) is widely used worldwide and one of the top five most-used pesticides in the United States. However, 1,3-D is classified as a Group 2B carcinogen and understanding its environmental fat…
View article: Sequential exposure to anoxic/oxic conditions leads to biotransformation and detoxification of sitagliptin in urban hyporheic zones
Sequential exposure to anoxic/oxic conditions leads to biotransformation and detoxification of sitagliptin in urban hyporheic zones Open
Pharmaceuticals are increasingly recognized as contaminants of concern in aquatic environments. Sitagliptin, an antidiabetic drug that carries a trifluoromethyl group, which is a precursor of the persistent trifluoroacetic acid, is excrete…
View article: Survival strategies of supraglacial algae-dominated communities in the transition from high light to continual darkness on the Greenland Ice Sheet
Survival strategies of supraglacial algae-dominated communities in the transition from high light to continual darkness on the Greenland Ice Sheet Open
Glacier ice algae of the streptophyte genus Ancylonema live on glaciers globally, including the Greenland Ice Sheet, and bloom despite low temperatures, low nutrient availability, and very high light intensities. In polar regions, the long…
View article: Fractionating proteins with nitrite-reducing activity in “Candidatus Kuenenia stuttgartiensis” strain CSTR1
Fractionating proteins with nitrite-reducing activity in “Candidatus Kuenenia stuttgartiensis” strain CSTR1 Open
The anammox bacteria “ Candidatus Kuenenia stuttgartiensis” ( Ca. Kuenenia) are able to gain energy by combining ammonium and nitrite to produce nitrogen gas, which is an ecologically and technically significant activity process. In this r…
View article: Roles of acyl carrier proteins in ladderane fatty acid producing-organisms
Roles of acyl carrier proteins in ladderane fatty acid producing-organisms Open
Ladderanes are highly strained hydrocarbons consisting of two or more linearly concatenated cyclobutane rings. Strikingly, ladderane moieties are part of unique fatty acids and fatty alcohols that are exclusively found in the membrane lipi…
View article: <i>De novo</i>peptide databases enable protein-based stable isotope probing of microbial communities with up to species-level resolution
<i>De novo</i>peptide databases enable protein-based stable isotope probing of microbial communities with up to species-level resolution Open
Background Protein-based stable isotope probing (Protein-SIP) is a powerful approach that can directly link individual taxa to activity and substrate assimilation, elucidating metabolic pathways and trophic relationships within microbial c…
View article: Revealing taxonomy, activity, and substrate assimilation in mixed bacterial communities by GroEL-proteotyping-based stable isotope probing
Revealing taxonomy, activity, and substrate assimilation in mixed bacterial communities by GroEL-proteotyping-based stable isotope probing Open
Protein-based stable isotope probing (protein-SIP) can link microbial taxa to substrate assimilation. Traditionally, protein-SIP requires a sample-specific metagenome-derived database for samples with unknown composition. Here, we describe…
View article: Reductive dehalogenase of Dehalococcoides mccartyi strain CBDB1 reduces cobalt- containing metal complexes enabling anodic respiration
Reductive dehalogenase of Dehalococcoides mccartyi strain CBDB1 reduces cobalt- containing metal complexes enabling anodic respiration Open
Microorganisms capable of direct or mediated extracellular electron transfer (EET) have garnered significant attention for their various biotechnological applications, such as bioremediation, metal recovery, wastewater treatment, energy ge…
View article: A Novel Sulfatase for Acesulfame Degradation in Wastewater Treatment Plants as Evidenced from <i>Shinella</i> Strains
A Novel Sulfatase for Acesulfame Degradation in Wastewater Treatment Plants as Evidenced from <i>Shinella</i> Strains Open
The artificial sweetener acesulfame is a persistent pollutant in wastewater worldwide. So far, only a few bacterial isolates were recently found to degrade acesulfame efficiently. In Bosea and Chelatococcus strains, a Mn2+-dependent metall…
View article: MecE, MecB, and MecC proteins orchestrate methyl group transfer during dichloromethane fermentation
MecE, MecB, and MecC proteins orchestrate methyl group transfer during dichloromethane fermentation Open
Dichloromethane (DCM), a common hazardous industrial chemical, is anaerobically metabolized by four bacterial genera: Dehalobacter , Dehalobacterium , Ca . Dichloromethanomonas, and Ca . Formimonas. However, the pivotal methyltransferases …
View article: Systems biology of electrogenic Pseudomonas putida - multi-omics insights and metabolic engineering for enhanced 2-ketogluconate production
Systems biology of electrogenic Pseudomonas putida - multi-omics insights and metabolic engineering for enhanced 2-ketogluconate production Open
Background Pseudomonas putida KT2440 has emerged as a promising host for industrial bioproduction. However, its strictly aerobic nature limits the scope of applications. Remarkably, this microbe exhibits high bioconversion efficiency when …
View article: Sequential Anaerobic–Aerobic Treatment Enhances Sulfamethoxazole Removal: From Batch Cultures to Observations in a Large-Scale Wastewater Treatment Plant
Sequential Anaerobic–Aerobic Treatment Enhances Sulfamethoxazole Removal: From Batch Cultures to Observations in a Large-Scale Wastewater Treatment Plant Open
Sulfamethoxazole (SMX) passes through conventional wastewater treatment plants (WWTPs) mainly unaltered. Under anoxic conditions sulfate-reducing bacteria can transform SMX but the fate of the transformation products (TPs) and their preval…
View article: Simultaneous ammonium removal and methane production against nitrite inhibition by coupling anammox bacteria activity in biogas digester
Simultaneous ammonium removal and methane production against nitrite inhibition by coupling anammox bacteria activity in biogas digester Open
Biogas fermentation is a widely used technology of commercial and environmental importance, but the presence of nitrogenous compounds can strongly impact reactor productivity. Here, we investigated methane production in the presence of nit…
View article: A novel sulfatase for acesulfame degradation in wastewater treatment plants as evidenced from<i>Shinella</i>strains
A novel sulfatase for acesulfame degradation in wastewater treatment plants as evidenced from<i>Shinella</i>strains Open
The artificial sweetener acesulfame is a persistent pollutant in wastewater worldwide. So far, only a few bacterial isolates were recently found to degrade acesulfame efficiently. In Bosea and Chelatococcus strains, a Mn 2+ -dependent meta…
View article: Escherichia coli adapts metabolically to 6- and 7-fluoroindole, enabling proteome-wide fluorotryptophan substitution
Escherichia coli adapts metabolically to 6- and 7-fluoroindole, enabling proteome-wide fluorotryptophan substitution Open
Nature has scarcely evolved a biochemistry around fluorine. However, modern science has shown that fluorinated organic molecules are suitable building blocks for biopolymers, from peptides and proteins up to entire organisms. Here, we cond…
View article: Dehalococcoides mccartyi strain CBDB1 takes up protons from the cytoplasm to reductively dehalogenate organohalides indicating a new modus of proton motive force generation
Dehalococcoides mccartyi strain CBDB1 takes up protons from the cytoplasm to reductively dehalogenate organohalides indicating a new modus of proton motive force generation Open
Proton translocation across the cytoplasmic membrane is a vital process for all organisms. Dehalococcoides strains are strictly anaerobic organohalide respiring bacteria that lack quinones and cytochromes but express a large membrane-bound…
View article: Characterization of phage vB_EcoS-EE09 infecting E. coli DSM613 Isolated from Wastewater Treatment Plant Effluent and Comparative Proteomics of the Infected and Non-Infected Host
Characterization of phage vB_EcoS-EE09 infecting E. coli DSM613 Isolated from Wastewater Treatment Plant Effluent and Comparative Proteomics of the Infected and Non-Infected Host Open
Phages influence microbial communities, can be applied in phage therapy, or may serve as bioindicators, e.g., in (waste)water management. We here characterized the Escherichia phage vB_EcoS-EE09 isolated from an urban wastewater treatment …
View article: GroEL-Proteotyping of Bacterial Communities Using Tandem Mass Spectrometry
GroEL-Proteotyping of Bacterial Communities Using Tandem Mass Spectrometry Open
Profiling bacterial populations in mixed communities is a common task in microbiology. Sequencing of 16S small subunit ribosomal-RNA (16S rRNA) gene amplicons is a widely accepted and functional approach but relies on amplification primers…
View article: <i>Escherichia coli</i>adapts metabolically to 6- and 7-fluoroindole, enabling proteome-wide fluorotryptophan substitution
<i>Escherichia coli</i>adapts metabolically to 6- and 7-fluoroindole, enabling proteome-wide fluorotryptophan substitution Open
Nature has scarcely evolved a biochemistry around fluorine. However, modern science proved fluorinated organic molecules to be suitable building blocks for biopolymers, from peptides and proteins up to entire organisms. Here, we conducted …
View article: An Efficient and Scalable Method for the Production of Immunogenic SARS-CoV-2 Virus-like Particles (VLP) from a Mammalian Suspension Cell Line
An Efficient and Scalable Method for the Production of Immunogenic SARS-CoV-2 Virus-like Particles (VLP) from a Mammalian Suspension Cell Line Open
The rapid evolution of new SARS-CoV-2 variants poses a continuing threat to human health. Vaccination has become the primary therapeutic intervention. The goal of the current work was the construction of immunogenic virus-like particles (V…
View article: Yeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamide synthesis on elongation factor 2
Yeast gene KTI13 (alias DPH8) operates in the initiation step of diphthamide synthesis on elongation factor 2 Open
In yeast, Elongator-dependent tRNA modifications are regulated by the Kti11•Kti13 dimer and hijacked for cell killing by zymocin, a tRNase ribotoxin. Kti11 (alias Dph3) also controls modification of elongation factor 2 (EF2) with diphthami…
View article: Recently evolved combination of unique sulfatase and amidase genes enables bacterial degradation of the wastewater micropollutant acesulfame worldwide
Recently evolved combination of unique sulfatase and amidase genes enables bacterial degradation of the wastewater micropollutant acesulfame worldwide Open
Xenobiotics often challenge the principle of microbial infallibility. One example is acesulfame introduced in the 1980s as zero-calorie sweetener, which was recalcitrant in wastewater treatment plants until the early 2010s. Then, efficient…
View article: Respiratory protein interactions in <scp> <i>Dehalobacter</i> </scp> sp. strain <scp>8M</scp> revealed through genomic and native proteomic analyses
Respiratory protein interactions in <span> <i>Dehalobacter</i> </span> sp. strain <span>8M</span> revealed through genomic and native proteomic analyses Open
Dehalobacter ( Firmicutes ) encompass obligate organohalide‐respiring bacteria used for bioremediation of groundwater contaminated with halogenated organics. Various aspects of their biochemistry remain unknown, including the identities an…
View article: GroEL-proteotyping of bacterial communities by tandem mass spectrometry
GroEL-proteotyping of bacterial communities by tandem mass spectrometry Open
Profiling bacterial populations in mixed communities is a common task in microbiology. 16 S rRNA-based sequencing is a widely accepted and functional approach but relies on amplification primers and cannot quantify isotope incorporation. T…
View article: Spatial organization and proteome of a dual-species cyanobacterial biofilm alter among N <sub>2</sub> -fixing and non-fixing conditions
Spatial organization and proteome of a dual-species cyanobacterial biofilm alter among N <sub>2</sub> -fixing and non-fixing conditions Open
Many disciplines have become increasingly interested in cyanobacteria, due to their ability to fix CO 2 while using water and sunlight as electron and energy sources. Further, several species of cyanobacteria are also capable of fixing mol…