Lorenzo Moroni
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View article: In vitro assessment of pirfenidone anti-fibrotic properties in a humanized 3D bioprinted kidney tubulointerstitium model
In vitro assessment of pirfenidone anti-fibrotic properties in a humanized 3D bioprinted kidney tubulointerstitium model Open
View article: A versatile device for in vitro magnetomechanical scaffold stimulation and enhancement of osteochondral differentiation of hMSCs
A versatile device for in vitro magnetomechanical scaffold stimulation and enhancement of osteochondral differentiation of hMSCs Open
Physical stimuli have received significant attention, owing to their capacity to create more biomimetic niches. While dynamic mechanical loading has shown promise in promoting osteogenic and chondrogenic differentiation, magnetic fields ha…
View article: Embedded 3D Printing of Graphene Oxide‐Containing, Chemically Crosslinkable Poly(Ethylene Glycol) Inks
Embedded 3D Printing of Graphene Oxide‐Containing, Chemically Crosslinkable Poly(Ethylene Glycol) Inks Open
The incorporation of graphene‐based materials into hydrogels enhances their mechanical, electroconductive, and antimicrobial properties, offering significant potential for biomedical applications. However, 3D printing graphene‐containing i…
View article: Soft Scaffolds for Nerve Repair: Investigating Glycerol‐Plasticized Chitosan Microstructures With In Vitro Complex Models
Soft Scaffolds for Nerve Repair: Investigating Glycerol‐Plasticized Chitosan Microstructures With In Vitro Complex Models Open
Peripheral nerve injuries are a significant clinical concern, often resulting in incomplete functional recovery due to the limitations of current treatments. Biomaterial‐based scaffolds that mimic the mechanical and topographical features …
View article: Bioprinted Constructs in the Regulatory Landscape: Current State and Future Perspectives
Bioprinted Constructs in the Regulatory Landscape: Current State and Future Perspectives Open
Bioprinting has become one of the leading topics in biomedical research in the past decade, as demonstrated by the great surge in publications and proliferation of bioprinting facilities worldwide. Bioprinting has gained widespread popular…
View article: Engineering antithrombogenic surfaces in synthetic small-diameter vascular grafts: A review of passive strategies
Engineering antithrombogenic surfaces in synthetic small-diameter vascular grafts: A review of passive strategies Open
Small-diameter vascular grafts are indispensable in coronary and peripheral artery bypass surgeries to reestablish the blood flow when blood vessels block. Despite advances in biomaterials' design, current clinical practice still relies he…
View article: Revealing the Auxetic Behavior of Biomimetic Multimaterial and Region‐Specific Nanofibrous Fascicle‐Inspired Scaffolds via Synchrotron Multiscale Digital Volume Correlation: Innovative Building Blocks for the Enthesis Regeneration
Revealing the Auxetic Behavior of Biomimetic Multimaterial and Region‐Specific Nanofibrous Fascicle‐Inspired Scaffolds via Synchrotron Multiscale Digital Volume Correlation: Innovative Building Blocks for the Enthesis Regeneration Open
Enthesis lesions are one of the prevalent causes of injuries in the tendon tissue. The gradient of mineralization, extracellular matrix organization and auxetic mechanical properties, make enthesis regeneration challenging. Innovative elec…
View article: Dimethyl Sulfoxide Conditions Induced Pluripotent Stem Cells for more Efficient Nephron Progenitor and Kidney Organoid Differentiation
Dimethyl Sulfoxide Conditions Induced Pluripotent Stem Cells for more Efficient Nephron Progenitor and Kidney Organoid Differentiation Open
View article: Packed for Ossification: High-Density Bioprinting of hPDC Spheroids in HAMA for Endochondral Ossification
Packed for Ossification: High-Density Bioprinting of hPDC Spheroids in HAMA for Endochondral Ossification Open
Long bone fractures are primarily repaired through endochondral ossification, a process in which a soft cartilage template forms at the injury site and is gradually replaced by bone. While bone has an innate self-healing capacity, this pro…
View article: The Route to Artery Mimetics: Hybrid Bioinks for Embedded Bioprinting of Multimaterial Cylindrical Models
The Route to Artery Mimetics: Hybrid Bioinks for Embedded Bioprinting of Multimaterial Cylindrical Models Open
The integration of biomaterials with living cells and stimuli‐responsive materials can be employed to create bioinks capable of generating 3D in vitro models that better recapitulate native tissues. A cellularized, multilayered cylindrical…
View article: Xanthan Gum–Iron System: Natural, Mechanically Tunable, Bioactive, and Magnetic-Responsive Hydrogels for Biomedical Engineering Applications
Xanthan Gum–Iron System: Natural, Mechanically Tunable, Bioactive, and Magnetic-Responsive Hydrogels for Biomedical Engineering Applications Open
Xanthan gum (XG) has performed far better than other polysaccharides for industrial purposes, e.g., food, pharmaceutical, and cosmetic applications, due to its outstanding thickening effect, pseudoplastic rheological properties, and non-to…
View article: Mechanical Reinforced and Self-healing Hydrogels: Bioprinted Biomimetic Methacrylated Collagen Peptide-Xanthan Gum Constructs for Ligament Regeneration.
Mechanical Reinforced and Self-healing Hydrogels: Bioprinted Biomimetic Methacrylated Collagen Peptide-Xanthan Gum Constructs for Ligament Regeneration. Open
Collagen peptide (COP) is water soluble, bioactive, and tends to be a promising alternative to collagen for tissue regeneration. However, its low viscosity and lack of readily polymerizable groups hinder its bioprinting and limit its wide …
View article: GIPPO: A Graph-based, Iterative, Printing-Path Optimization Slicer for Architected Lattices
GIPPO: A Graph-based, Iterative, Printing-Path Optimization Slicer for Architected Lattices Open
Architected materials of significant geometric complexity offer exceptional mechanical properties that often surpass those of their constituent materials. However, their fabrication through extrusion-based 3D printing remains hindered by s…
View article: Experimentally-informed in silico design of melt-electrowritten scaffolds for tissue engineering applications
Experimentally-informed in silico design of melt-electrowritten scaffolds for tissue engineering applications Open
View article: Marine-derived sulfated polysaccharides enhance hemocompatibility and endothelialization of nanofibrous PCL for vascular graft applications
Marine-derived sulfated polysaccharides enhance hemocompatibility and endothelialization of nanofibrous PCL for vascular graft applications Open
View article: Electrospun Polymer Fiber Mats for Persulfide Prodrug Delivery
Electrospun Polymer Fiber Mats for Persulfide Prodrug Delivery Open
Persulfides (RSSH) and hydrogen sulfide (H2S) are endogenously produced bioactive molecules that serve important roles in vasculogenesis and tissue repair processes. Through the physical incorporation of RSSH/H2S dono…
View article: Geometrical constraints dictate assembly and phenotype of human iPSC-derived motoneuronal spheroids
Geometrical constraints dictate assembly and phenotype of human iPSC-derived motoneuronal spheroids Open
Our approach enables the scalable production of size-controlled MNP spheroids and underscores the effect of geometrical confinement on regulating motor neuron differentiation.
View article: Acoustically Responsive Nanofibrous Scaffolds with 3D Hierarchy for Tympanic Membrane Regeneration
Acoustically Responsive Nanofibrous Scaffolds with 3D Hierarchy for Tympanic Membrane Regeneration Open
Mesenchymal stromal cell (MSC)‐based therapies have emerged as a promising approach for repairing tympanic membrane (TM) injuries. However, the role of essential biochemical, biophysical, and biomechanical cues in guiding MSC differentiati…
View article: A Tissue Engineering's Guide to Biomimicry
A Tissue Engineering's Guide to Biomimicry Open
Biomimicry is a broadly used term in many fields such as in architecture and industrial design to pharmacology and many others. Biomimicry tries to replicate a product or process that occurs in the natural environment. However, due to the …
View article: 3D bioprinting in tissue engineering: current state-of-the-art and challenges towards system standardization and clinical translation
3D bioprinting in tissue engineering: current state-of-the-art and challenges towards system standardization and clinical translation Open
Over the past decade, three-dimensional (3D) bioprinting has made significant progress, transforming into a key innovation in tissue engineering. Despite the early strides, critical challenges remain in 3D bioprinting that must be addresse…
View article: Review of Disruptive Technologies in 3D Bioprinting
Review of Disruptive Technologies in 3D Bioprinting Open
Purpose of Review The purpose of this review is to share insights from recognized experts in 3D biopriniting on the recent advances in these technologies discussed during a recent workshop held in conjunction with the 2024 ISS National Lab…
View article: Microfluidic bioprinting as a tool to produce hiPSCs-derived renal organoids
Microfluidic bioprinting as a tool to produce hiPSCs-derived renal organoids Open
Chronic kidney disease affects 10% of the global population and often progresses to end-stage renal disease, where dialysis or renal transplant are the only therapies, though neither is a permanent solution. Regenerative medicine, particul…
View article: Pericyte‐Assisted Vascular Lumen Organization in a Novel Dynamic Human Blood‐Brain Barrier‐on‐Chip Model
Pericyte‐Assisted Vascular Lumen Organization in a Novel Dynamic Human Blood‐Brain Barrier‐on‐Chip Model Open
Organ‐on‐Chip (OoC) technology provides a powerful platform for neurovascular research, enabling the precise replication of the blood‐brain barrier (BBB) microenvironment, including its 3D architecture and the influence of dynamic blood fl…
View article: RNA extraction from cells, spheroids, or organoids encapsulated in alginate norbornene using Maxwell® RSC Plant RNA Kit v1
RNA extraction from cells, spheroids, or organoids encapsulated in alginate norbornene using Maxwell® RSC Plant RNA Kit v1 Open
This protocol details the isolation of RNA from cells, spheroids, or organoids encapsulated in alginate norbornene hydrogels using the Maxwell® RSC Plant RNA Kit and automated Maxwell® RSC instrument. The cells, spheroids, or organoids are…
View article: The Importance of Coating Surface and Composition for Attachment and Survival of Neuronal Cells Under Mechanical Stimulation
The Importance of Coating Surface and Composition for Attachment and Survival of Neuronal Cells Under Mechanical Stimulation Open
Cell culture of neuronal cells places high demands on the surface for these cells to adhere to and grow on. Native extracellular matrix (ECM) proteins are often applied to the cell culture surface. The substrate is even more important when…
View article: Understanding the structure and mechanics of the sheep calcaneal enthesis: a relevant animal model to design scaffolds for tissue engineering applications
Understanding the structure and mechanics of the sheep calcaneal enthesis: a relevant animal model to design scaffolds for tissue engineering applications Open
View article: Hybrid bioinks for embedded bioprinting of an artery model
Hybrid bioinks for embedded bioprinting of an artery model Open
The integration of biomaterials with living cells and stimuli-responsive materials can be employed to create bioinks capable of generating 3D in vitro models that better recapitulate native tissues. We introduce a multilayered artery model…
View article: Designing Dynamic Hydrogels: The Interplay of Cross-Linker Length, Valency, and Reaction Kinetics in Hydrazone-Based Networks
Designing Dynamic Hydrogels: The Interplay of Cross-Linker Length, Valency, and Reaction Kinetics in Hydrazone-Based Networks Open
Hydrogels designed using dynamic (reversible) chemistry are prominent tools in diverse research areas as they grant access to time-dependent mechanical properties (self-healing and viscoelasticity), which are inaccessible via purely covale…
View article: Bioprinting of Alginate-Norbornene bioinks to create a versatile platform for kidney in vitro modeling
Bioprinting of Alginate-Norbornene bioinks to create a versatile platform for kidney in vitro modeling Open
Chronic kidney diseases affect a significant portion of the global population and their prevalence is expected to increase in the coming years. Advanced in vitro models are crucial for understanding disease onset and for improving d…
View article: RNA extraction from organoids encapsulated in alginate norbornene using TRIzol Reagent v1
RNA extraction from organoids encapsulated in alginate norbornene using TRIzol Reagent v1 Open
This protocol describes the isolation of high-quality RNA from organoids encapsulated in alginate norbornene hydrogels. Organoids are first released from the hydrogel matrix using alginate lyase digestion, followed by homogenization in TRI…