Lorraine Eley
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View article: Remodelling of supernumerary leaflet primordia leads to bicuspid aortic valve caused by loss of primary cilia
Remodelling of supernumerary leaflet primordia leads to bicuspid aortic valve caused by loss of primary cilia Open
Aims Bicuspid aortic valve (BAV), where two valve leaflets are found instead of the usual three, affects 1–2% of the general population and is associated with significant morbidity and mortality. Despite its frequency, the majority of case…
View article: Remodelling of supernumerary leaflet primordia leads to bicuspid aortic valve (BAV) caused by loss of primary cilia
Remodelling of supernumerary leaflet primordia leads to bicuspid aortic valve (BAV) caused by loss of primary cilia Open
Aims Bicuspid aortic valve (BAV), where two valve leaflets are found instead of the usual three, affects 1-2% of the general population and is associated with significant morbidity and mortality. Despite its frequency, the majority of case…
View article: Beyond genomic studies of congenital heart defects through systematic modelling and phenotyping
Beyond genomic studies of congenital heart defects through systematic modelling and phenotyping Open
Congenital heart defects (CHDs), the most common congenital anomalies, are considered to have a significant genetic component. However, despite considerable efforts to identify pathogenic genes in patients with CHDs, few gene variants have…
View article: Zebrafish arterial valve development occurs through direct differentiation of second heart field progenitors
Zebrafish arterial valve development occurs through direct differentiation of second heart field progenitors Open
Aims Bicuspid aortic valve (BAV) is the most common congenital heart defect, affecting at least 2% of the population. The embryonic origins of BAV remain poorly understood, with few assays for validating patient variants, limiting the iden…
View article: Zebrafish arterial valve development occurs through direct differentiation of second heart field progenitors
Zebrafish arterial valve development occurs through direct differentiation of second heart field progenitors Open
Aims Bicuspid Aortic Valve (BAV) is the most common congenital heart defect, affecting at least 2% of the population. The embryonic origins of BAV remain poorly understood, limiting the identification of assays for validating patient varia…
View article: eNOS plays essential roles in the developing heart and aorta linked to disruption of Notch signalling
eNOS plays essential roles in the developing heart and aorta linked to disruption of Notch signalling Open
eNOS (NOS3) is the enzyme that generates nitric oxide, a signalling molecule and regulator of vascular tone. Loss of eNOS function is associated with increased susceptibility to atherosclerosis, hypertension, thrombosis and stroke. Aortopa…
View article: Spatial transcriptomics reveals novel genes during the remodelling of the embryonic human arterial valves
Spatial transcriptomics reveals novel genes during the remodelling of the embryonic human arterial valves Open
Abnormalities of the arterial valves, including bicuspid aortic valve (BAV) are amongst the most common congenital defects and are a significant cause of morbidity as well as predisposition to disease in later life. Despite this, and compo…
View article: Development of the arterial roots and ventricular outflow tracts
Development of the arterial roots and ventricular outflow tracts Open
The separation of the outflow tract of the developing heart into the systemic and pulmonary arterial channels remains controversial and poorly understood. The definitive outflow tracts have three components. The developing outflow tract, i…
View article: Functional analysis of germline <i>VANGL2</i> variants using rescue assays of <i>vangl2</i> knockout zebrafish
Functional analysis of germline <i>VANGL2</i> variants using rescue assays of <i>vangl2</i> knockout zebrafish Open
Developmental studies have shown that the evolutionarily conserved Wnt Planar Cell Polarity (PCP) pathway is essential for the development of a diverse range of tissues and organs including the brain, spinal cord, heart and sensory organs,…
View article: Spatial transcriptomics reveals novel genes during the remodelling of the embryonic human arterial valves
Spatial transcriptomics reveals novel genes during the remodelling of the embryonic human arterial valves Open
Abnormalities of the arterial valves, including bicuspid aortic valve (BAV) are amongst the most common congenital defects and are a significant cause of morbidity as well as predisposition to disease in later life. Despite this, and compo…
View article: The Left Ventricular Myocardium in Hypoplastic Left Heart Syndrome
The Left Ventricular Myocardium in Hypoplastic Left Heart Syndrome Open
Hypoplastic left heart syndrome (HLHS) is a collective term applied to severe congenital cardiac malformations, characterised by a combination of abnormalities mainly affecting the left ventricle, associated valves, and ascending aorta. Al…
View article: Sequential action of JNK genes establishes the embryonic left-right axis
Sequential action of JNK genes establishes the embryonic left-right axis Open
The establishment of the left-right axis is crucial for the placement, morphogenesis and function of internal organs. Left-right specification is proposed to be dependent on cilia-driven fluid flow in the embryonic node. Planar cell polari…
View article: Development of the Human Arterial Valves: Understanding Bicuspid Aortic Valve
Development of the Human Arterial Valves: Understanding Bicuspid Aortic Valve Open
Abnormalities in the arterial valves are some of the commonest congenital malformations, with bicuspid aortic valve (BAV) occurring in as many as 2% of the population. Despite this, most of what we understand about the development of the a…
View article: Exercise, programmed cell death and exhaustion of cardiomyocyte proliferation in aging zebrafish
Exercise, programmed cell death and exhaustion of cardiomyocyte proliferation in aging zebrafish Open
Exercise may ameliorate the eventual heart failure inherent in human aging. In this study, we use zebrafish to understand how aging and exercise affect cardiomyocyte turnover and myocardial remodelling. We show that cardiomyocyte prolifera…
View article: New Concepts in the Development and Malformation of the Arterial Valves
New Concepts in the Development and Malformation of the Arterial Valves Open
Although in many ways the arterial and atrioventricular valves are similar, both being derived for the most part from endocardial cushions, we now know that the arterial valves and their surrounding structures are uniquely dependent on pro…
View article: Isolation and next generation sequencing of archival formalin‐fixed DNA
Isolation and next generation sequencing of archival formalin‐fixed DNA Open
DNA from archived organs is presumed unsuitable for genomic studies because of excessive formalin‐fixation. As next generation sequencing (NGS) requires short DNA fragments, and Uracil‐N‐glycosylase (UNG) can be used to overcome deaminatio…
View article: Alternative splicing of jnk1a in zebrafish determines first heart field ventricular cardiomyocyte numbers through modulation of hand2 expression
Alternative splicing of jnk1a in zebrafish determines first heart field ventricular cardiomyocyte numbers through modulation of hand2 expression Open
The planar cell polarity pathway is required for heart development and whilst the functions of most pathway members are known, the roles of the jnk genes in cardiac morphogenesis remain unknown as mouse mutants exhibit functional redundanc…
View article: Co-Morbidity of Irritable Bowel Syndrome and Chronic Pelvic Pain: a One-Year Follow-Up Study
Co-Morbidity of Irritable Bowel Syndrome and Chronic Pelvic Pain: a One-Year Follow-Up Study Open
Background: Significant co-morbidity between Irritable Bowel Syndrome (IBS) and chronic pelvic pain (CPP) has been reported (35-39%). IBS affects 10-20% of the general population, and is the most common condition diagnosed by gastroenterol…
View article: Development and maturation of the fibrous components of the arterial roots in the mouse heart
Development and maturation of the fibrous components of the arterial roots in the mouse heart Open
The arterial roots are important transitional regions of the heart, connecting the intrapericardial components of the aortic and pulmonary trunks with their ventricular outlets. They house the arterial (semilunar) valves and, in the case o…
View article: Wound healing assays on cell migration and localization of focal adhesions in growth-arrested <i>inv<sup>+/+</sup></i> and <i>inv<sup>−/−</sup></i> MEFs.
Wound healing assays on cell migration and localization of focal adhesions in growth-arrested <i>inv<sup>+/+</sup></i> and <i>inv<sup>−/−</sup></i> MEFs. Open
Graphs of time-lapse data represent mean values of four independent experiments ± S.E.M. (p<0.001, one-way ANOVA). Cells were serum starved for 24 h and allowed to recover 1 h post wounding, hence t = 0 h in the data sets relates to the i…