Luca Dolfi
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View article: Analysis of microRNA expression reveals convergent evolution of the molecular control of diapause in annual killifishes
Analysis of microRNA expression reveals convergent evolution of the molecular control of diapause in annual killifishes Open
Background Diapause is a condition of developmental arrest in anticipation of adverse environmental conditions present in many diverse taxa. Diapause is a key adaptation that enabled the colonization of ephemeral habitats subject to the al…
View article: Refeeding-associated AMPKγ1 complex activity is a hallmark of health and longevity
Refeeding-associated AMPKγ1 complex activity is a hallmark of health and longevity Open
Late-life-initiated dietary interventions show limited efficacy in extending longevity or mitigating frailty, yet the underlying causes remain unclear. Here we studied the age-related fasting response of the short-lived killifish Nothobran…
View article: In Vitro Fertilization of the African Turquoise Killifish<i>Nothobranchius furzeri</i>
In Vitro Fertilization of the African Turquoise Killifish<i>Nothobranchius furzeri</i> Open
The ability to perform in vitro fertilization, together with sperm cryopreservation, greatly facilitates the long-term laboratory maintenance of wild-type and transgenic model organisms and helps prevent genetic drift. It is also useful in…
View article: A computational framework for testing hypotheses of the minimal mechanical requirements for cell aggregation using early annual killifish embryogenesis as a model
A computational framework for testing hypotheses of the minimal mechanical requirements for cell aggregation using early annual killifish embryogenesis as a model Open
Introduction: Deciphering the biological and physical requirements for the outset of multicellularity is limited to few experimental models. The early embryonic development of annual killifish represents an almost unique opportunity to inv…
View article: Nonlethal Blood Sampling from the Killifish<i>Nothobranchius furzeri</i>
Nonlethal Blood Sampling from the Killifish<i>Nothobranchius furzeri</i> Open
Blood withdrawal is a common procedure performed on laboratory animals to monitor key processes and indicators of fish health and physiology, such as hematopoiesis, hemostasis, and lipid and glucose metabolism. Moreover, the ability to ext…
View article: Refeeding-associated AMPK<sub>γ1</sub>complex activity is a hallmark of health and longevity
Refeeding-associated AMPK<sub>γ1</sub>complex activity is a hallmark of health and longevity Open
Late-life-initiated dietary interventions negligibly extend longevity or reduce frailty, yet the reason remains unknown. We investigated the age-related changes associated with the fasting response in adipose tissue of the short-lived kill…
View article: Analysis of microRNA expression reveals convergent evolution of the molecular control of diapause in annual fish
Analysis of microRNA expression reveals convergent evolution of the molecular control of diapause in annual fish Open
BackgroundDiapause is a key adaptation that enabled the colonization of ephemeral habitats subject to the alternation of dry and wet seasons by annual killifishes. Upon desiccation of the ponds, killifish embryos remain vital but quiescent…
View article: Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri
Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri Open
Over the last decade, the African turquoise killifish, Nothobranchius furzeri, has emerged as an important model system for the study of vertebrate biology and ageing. N. furzeri propagation, however, can pose challenges due to the short w…
View article: Optimization of cryopreservation and<i>in vitro</i>fertilization techniques for the African turquoise killifish<i>Nothobranchius furzeri</i>
Optimization of cryopreservation and<i>in vitro</i>fertilization techniques for the African turquoise killifish<i>Nothobranchius furzeri</i> Open
Over the last decade, the African turquoise killifish, Nothobranchius furzeri , has emerged as an important model system for the study of vertebrate biology and ageing. However, rearing this fish in captivity can pose challenges, due to th…
View article: Cell cycle dynamics during diapause entry and exit in an annual killifish revealed by FUCCI technology
Cell cycle dynamics during diapause entry and exit in an annual killifish revealed by FUCCI technology Open
Background Annual killifishes are adapted to surviving and reproducing over alternating dry and wet seasons. During the dry season, all adults die and desiccation-resistant embryos remain encased in dry mud for months or years in a state o…
View article: MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging
MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging Open
Our results demonstrate that age-dependent miR-29 upregulation is an adaptive mechanism that counteracts the expression of some aging-related phenotypes and its anti-aging activity is primarily exerted by regulating intracellular iron home…
View article: Additional file 11: of MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging
Additional file 11: of MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging Open
Supporting Data: Raw quantitative PCR data relative to Figs. 1b and 6câ e, g, h. (XLSX 50 kb)
View article: Additional file 5: of MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging
Additional file 5: of MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging Open
Complete list of genes coherently or incoherently regulated by aging and sponge-29. (XLSX 43 kb)
View article: Additional file 3: of MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging
Additional file 3: of MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging Open
RNA-seq data analysis (DEG list). (XLSX 156 kb)
View article: MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging
MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging Open
Iron is an essential metal cofactor for enzymes involved in many cellular functions such as energy generation and cell proliferation. However, excessive iron concentration leads to increased oxidative stress and toxicity. As such, iron hom…
View article: MicroRNAs provide the first evidence of genetic link between diapause and aging in vertebrates
MicroRNAs provide the first evidence of genetic link between diapause and aging in vertebrates Open
Summary Diapause and aging are controlled by overlapping genetic mechanisms in C.elegans and these include microRNAs (miRNAs). Here, we investigated miRNA regulation in embryos of annual killifish that naturally undergo diapause to overcom…