Luca Regazzoni
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View article: In situ-generated volumetric dried plasma spots for the analysis of edaravone and metabolites in animal models
In situ-generated volumetric dried plasma spots for the analysis of edaravone and metabolites in animal models Open
View article: Fractional Coprecipitation of Drugs and Natural Extracts with Zinc Hydroxide
Fractional Coprecipitation of Drugs and Natural Extracts with Zinc Hydroxide Open
Zinc hydroxide has been reported as an effective precipitating reagent for removing proteins in biological samples. This procedure is quite effective for removing interfering proteins before the chromatographic separation of small organic …
View article: Protein Precipitation by Metal Hydroxides as a Convenient and Alternative Sample Preparation Procedure for Bioanalysis
Protein Precipitation by Metal Hydroxides as a Convenient and Alternative Sample Preparation Procedure for Bioanalysis Open
Protein precipitation is widely used for sample preparation ahead of liquid chromatography. This step is required to analyze small molecules without the interference of proteins contained in the matrix. Organic solvents and acidic chemical…
View article: Exploring Secondary Amine Carnosine Derivatives: Design, Synthesis, and Properties
Exploring Secondary Amine Carnosine Derivatives: Design, Synthesis, and Properties Open
Carnosine is a naturally occurring dipeptide that has been advocated by some authors as an interesting scaffold for the development of potential therapeutic agents in view of the positive outcomes of its supplementation in animal models of…
View article: State of the Art in the Development of Human Serum Carnosinase Inhibitors
State of the Art in the Development of Human Serum Carnosinase Inhibitors Open
Human serum carnosinase is an enzyme that operates the preferential hydrolysis of dipeptides with a C-terminus histidine. Only higher primates excrete such an enzyme in serum and cerebrospinal fluid. In humans, the serum hydrolytic rate ha…
View article: Protein Precipitation by Transition Metal Hydroxides as a Convenient and Alternative Sample Preparation Procedure for Bioanalysis
Protein Precipitation by Transition Metal Hydroxides as a Convenient and Alternative Sample Preparation Procedure for Bioanalysis Open
View article: A Green and Cost-Effective Chromatographic Method for the Measurement of the Activity of Human Serum Carnosinase
A Green and Cost-Effective Chromatographic Method for the Measurement of the Activity of Human Serum Carnosinase Open
Carnosinase (i.e., CN1; E.C. 3.4.13.20) is an enzyme found in the sera of higher primates. CN1 preferentially catalyzes the hydrolysis of natural, orally adsorbed histidine dipeptides like carnosine (i.e., β-alanyl-L-histidine). This is th…
View article: A Novel Chromatographic Method to Assess the Binding Ability towards Dicarbonyls
A Novel Chromatographic Method to Assess the Binding Ability towards Dicarbonyls Open
Human exposure to dicarbonyls occurs via ingestion (e.g., food), inhalation (e.g., electronic cigarettes) and dysregulation of endogenous metabolic pathways (e.g., glycolysis). Dicarbonyls are electrophiles able to induce carbonylation of …
View article: A Novel Chromatographic Method to Assess the Binding Ability towards Dicarbonyls
A Novel Chromatographic Method to Assess the Binding Ability towards Dicarbonyls Open
Dicarbonyls are electrophiles able to generate the so-called advanced glycation end-products (AGEs). Human exposure occurs via ingestion (e.g., food), inhalation (e.g., electronic cigarettes) and dysregulation of endogenous metabolic pathw…
View article: Volumetric Absorptive Microsampling (VAMS) for Targeted LC-MS/MS Determination of Tryptophan-Related Biomarkers
Volumetric Absorptive Microsampling (VAMS) for Targeted LC-MS/MS Determination of Tryptophan-Related Biomarkers Open
L-Tryptophan (TRP) metabolites and related biomarkers play crucial roles in physiological functions, and their imbalances are implicated in central nervous system pathologies and neurodegenerative diseases such as amyotrophic lateral scler…
View article: Chromatographic measurement of 3-hydroxyanthranilate 3,4-dioxygenase activity reveals that edaravone can mitigate the formation of quinolinic acid through a direct enzyme inhibition
Chromatographic measurement of 3-hydroxyanthranilate 3,4-dioxygenase activity reveals that edaravone can mitigate the formation of quinolinic acid through a direct enzyme inhibition Open
View article: Cyclo(His-Pro) Exerts Protective Carbonyl Quenching Effects through Its Open Histidine Containing Dipeptides
Cyclo(His-Pro) Exerts Protective Carbonyl Quenching Effects through Its Open Histidine Containing Dipeptides Open
Cyclo(His-Pro) (CHP) is a cyclic dipeptide which is endowed with favorable pharmacokinetic properties combined with a variety of biological activities. CHP is found in a number of protein-rich foods and dietary supplements. While being sta…
View article: The ergogenic effect of acute carnosine and anserine supplementation: dosing, timing, and underlying mechanism
The ergogenic effect of acute carnosine and anserine supplementation: dosing, timing, and underlying mechanism Open
These studies reveal that acute ingestion of 30 mg.kg-1 of both carnosine and anserine, 60 minutes before a high intensity exercise, can potentially improve performance, such as short cycling sprints or maximal muscle contractio…
View article: Liquid phase separation techniques for the characterization of monoclonal antibodies and bioconjugates
Liquid phase separation techniques for the characterization of monoclonal antibodies and bioconjugates Open
View article: Characterization of Catecholaldehyde Adducts with Carnosine and <scp>l</scp>-Cysteine Reveals Their Potential as Biomarkers of Catecholaminergic Stress
Characterization of Catecholaldehyde Adducts with Carnosine and <span>l</span>-Cysteine Reveals Their Potential as Biomarkers of Catecholaminergic Stress Open
[Image: see text] Monoamine oxidase (MAO) catalyzes the oxidative deamination of dopamine and norepinephrine to produce 3,4-dihydroxyphenylacetaldehyde (DOPAL) and 3,4-dihydroxyphenylglycolaldehyde (DOPEGAL), respectively. Both of these al…
View article: Synthesis and characterization of 13C labeled carnosine derivatives for isotope dilution mass spectrometry measurements in biological matrices
Synthesis and characterization of 13C labeled carnosine derivatives for isotope dilution mass spectrometry measurements in biological matrices Open
View article: Acute preexercise supplementation of combined carnosine and anserine enhances initial maximal power of Wingate tests in humans
Acute preexercise supplementation of combined carnosine and anserine enhances initial maximal power of Wingate tests in humans Open
Current results reveal that carnosine and anserine competitively bind to the highly active carnosinase enzyme in human plasma. Acute combined carnosine and anserine supplementation is therefore described as novel strategy to raise plasma a…
View article: Chromatographic Characterization and In Vitro Bioactivity Evaluation of Lactobacillus helveticus Hydrolysates upon Fermentation of Different Substrates
Chromatographic Characterization and In Vitro Bioactivity Evaluation of Lactobacillus helveticus Hydrolysates upon Fermentation of Different Substrates Open
Among various food sources, milk proteins remain the major vector for functional peptides endowed with several biological activities. Particularly, the proteolytic activity of lactic acid bacteria during milk fermentation has been one of t…
View article: Towards the Inhibition of Protein–Protein Interactions (PPIs) in STAT3: Insights into a New Class of Benzothiadiazole Derivatives
Towards the Inhibition of Protein–Protein Interactions (PPIs) in STAT3: Insights into a New Class of Benzothiadiazole Derivatives Open
Signal transducer and activator of transcription 3 (STAT3) is a validated anticancer target due to the relationship between its constitutive activation and malignant tumors. Through a virtual screening approach on the STAT3-SH2 domain, 5,6…
View article: Two novel fish paralogs provide insights into the Rid family of imine deaminases active in pre-empting enamine/imine metabolic damage
Two novel fish paralogs provide insights into the Rid family of imine deaminases active in pre-empting enamine/imine metabolic damage Open
View article: Dataset related to the article "Pro-oxidant and pro-inflammatory effects of glycated albumin on cardiomyocytes"
Dataset related to the article "Pro-oxidant and pro-inflammatory effects of glycated albumin on cardiomyocytes" Open
This record contains raw data related to the article "Pro-oxidant and pro-inflammatory effects of glycated albumin on cardiomyocytes" A B S T R A C T Human serum albumin (HSA) is the most abundant circulating protein in the body and presen…
View article: Dataset related to the article "Pro-oxidant and pro-inflammatory effects of glycated albumin on cardiomyocytes"
Dataset related to the article "Pro-oxidant and pro-inflammatory effects of glycated albumin on cardiomyocytes" Open
This record contains raw data related to the article "Pro-oxidant and pro-inflammatory effects of glycated albumin on cardiomyocytes" A B S T R A C T Human serum albumin (HSA) is the most abundant circulating protein in the body and presen…
View article: In Vitro Aging of Human Skin Fibroblasts: Age-Dependent Changes in 4-Hydroxynonenal Metabolism
In Vitro Aging of Human Skin Fibroblasts: Age-Dependent Changes in 4-Hydroxynonenal Metabolism Open
Evidence suggests that the increased production of free radicals and reactive oxygen species lead to cellular aging. One of the consequences is lipid peroxidation generating reactive aldehydic products, such as 4-hydroxynonenal (HNE) that …
View article: Tuning PFKFB3 Bisphosphatase Activity Through Allosteric Interference
Tuning PFKFB3 Bisphosphatase Activity Through Allosteric Interference Open
View article: Profiling Vaccinium macrocarpon components and metabolites in human urine and the urine ex-vivo effect on Candida albicans adhesion and biofilm-formation
Profiling Vaccinium macrocarpon components and metabolites in human urine and the urine ex-vivo effect on Candida albicans adhesion and biofilm-formation Open
View article: Differentially Expressed Proteins in Primary Endothelial Cells Derived From Patients With Acute Myocardial Infarction
Differentially Expressed Proteins in Primary Endothelial Cells Derived From Patients With Acute Myocardial Infarction Open
Endothelial dysfunction is one of the primary factors in the onset and progression of atherothrombosis resulting in acute myocardial infarction (AMI). However, the pathological and cellular mechanisms of endothelial dysfunction in AMI have…
View article: Synthesis and application of isotope-labeled carnosine in LCMS/MS
Synthesis and application of isotope-labeled carnosine in LCMS/MS Open
View article: Pro-oxidant and pro-inflammatory effects of glycated albumin on cardiomyocytes
Pro-oxidant and pro-inflammatory effects of glycated albumin on cardiomyocytes Open
View article: Ripe and Raw Pu-Erh Tea: LC-MS Profiling, Antioxidant Capacity and Enzyme Inhibition Activities of Aqueous and Hydro-Alcoholic Extracts
Ripe and Raw Pu-Erh Tea: LC-MS Profiling, Antioxidant Capacity and Enzyme Inhibition Activities of Aqueous and Hydro-Alcoholic Extracts Open
Herein, we reported a detailed profiling of soluble components of two fermented varieties of Chinese green tea, namely raw and ripe pu-erh. The identification and quantification of the main components was carried out by means of mass spect…
View article: Phenolic profile and biological activity of table grapes (<i>Vitis vinifera L</i>.)
Phenolic profile and biological activity of table grapes (<i>Vitis vinifera L</i>.) Open
Table grapes are largely produced and consumed in the Mediterranean area. Furthermore, in the last years, the commercial interest in table grapes and other non-alcoholic grape products has notably increased worldwide. In addition to the nu…