Lucas Waldburger
YOU?
Author Swipe
View article: Multiplexed profiling of transcriptional regulators in plant cells
Multiplexed profiling of transcriptional regulators in plant cells Open
Transcriptional regulators play key roles in plant growth, development and environmental responses; however, understanding how their regulatory activity is encoded at the protein level has been hindered by a lack of multiplexed large-scale…
View article: Author Correction: Binary vector copy number engineering improves Agrobacterium-mediated transformation
Author Correction: Binary vector copy number engineering improves Agrobacterium-mediated transformation Open
View article: Binary vector origin predictably determines <i>Agrobacterium</i> -mediated transformation outcome across eukaryotic kingdoms
Binary vector origin predictably determines <i>Agrobacterium</i> -mediated transformation outcome across eukaryotic kingdoms Open
Agrobacterium -mediated transformation (AMT) is the primary means of genetic engineering in plants and many fungi, but the factors that control transformation outcomes—efficiency, transgene insertion number, and transgene integrity—remain …
View article: Diversity-driven biochemical survey reveals dimeric structural origin of rubisco
Diversity-driven biochemical survey reveals dimeric structural origin of rubisco Open
Rubisco is the entry point of nearly all organic carbon into the biosphere and is present in all domains of life. Despite its global importance, biochemical studies of this enzyme superfamily have been limited to a relatively narrow set of…
View article: Engineering Polyketide Stereocenters with Ketoreductase Domain Exchanges
Engineering Polyketide Stereocenters with Ketoreductase Domain Exchanges Open
Polyketide synthases (PKSs) are versatile biosynthetic megasynthases capable of producing a diverse range of natural products with many applications, including in pharmaceuticals. The stereochemical precision of PKSs makes them a powerful …
View article: Evolutionary discovery and characterization of fungal transcriptional activators using active learning
Evolutionary discovery and characterization of fungal transcriptional activators using active learning Open
Biological discovery and design are increasingly being guided by predictive models in place of costly experimentation. However, existing datasets are often biased by overrepresentation from model organisms, leading to failures in evolution…
View article: Causes and consequences of experimental variation in <i>Nicotiana benthamiana</i> transient expression
Causes and consequences of experimental variation in <i>Nicotiana benthamiana</i> transient expression Open
Infiltration of Agrobacterium tumefaciens into Nicotiana benthamiana has become a foundational technique in plant biology, enabling efficient delivery of transgenes in planta with technical ease, robust signal, and relatively high throughp…
View article: Multiplexed profiling of transcriptional regulators in plant cells
Multiplexed profiling of transcriptional regulators in plant cells Open
Transcriptional regulators play key roles in plant growth, development, and environmental responses; however, understanding how their regulatory activity is encoded at the protein level has been hindered by a lack of multiplexed large–scal…
View article: Leveraging a synthetic biology approach to enhance BCG-mediated expansion of Vγ9Vδ2 T cells
Leveraging a synthetic biology approach to enhance BCG-mediated expansion of Vγ9Vδ2 T cells Open
There is an urgent need to develop a more efficacious anti-tuberculosis vaccine as the current live-attenuated vaccine strain BCG fails to prevent pulmonary infection in adults. Our long-term goal is to test whether increasing the immunoge…
View article: Binary vector copy number engineering improves Agrobacterium-mediated transformation
Binary vector copy number engineering improves Agrobacterium-mediated transformation Open
View article: Expression of a mammalian RNA demethylase increases flower number and floral stem branching in <scp> <i>Arabidopsis thaliana</i> </scp>
Expression of a mammalian RNA demethylase increases flower number and floral stem branching in <span> <i>Arabidopsis thaliana</i> </span> Open
RNA methylation plays a central regulatory role in plant biology and is a relatively new target for plant improvement efforts. In nearly all cases, perturbation of the RNA methylation machinery results in deleterious phenotypes. However, a…
View article: Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana
Expression of a mammalian RNA demethylase increases flower number and floral stem branching in Arabidopsis thaliana Open
RNA methylation plays a central regulatory role in plant biology and is a relatively new target for plant improvement efforts. In nearly all cases, perturbation of the RNA methylation machinery results in deleterious phenotypes. However, a…
View article: Genetically refactored<i>Agrobacterium</i>-mediated transformation
Genetically refactored<i>Agrobacterium</i>-mediated transformation Open
Members of Agrobacterium are costly plant pathogens while also essential tools for plant transformation. Though Agrobacterium -mediated transformation (AMT) has been heavily studied, its polygenic nature and its complex transcriptional reg…
View article: Transcriptome architecture of the three main lineages of agrobacteria
Transcriptome architecture of the three main lineages of agrobacteria Open
Agrobacteria are a diverse, polyphyletic group of prokaryotes with multipartite genomes capable of transferring DNA into the genomes of host plants, making them an essential tool in plant biotechnology. Despite their utility in plant trans…
View article: The pGinger Family of Expression Plasmids
The pGinger Family of Expression Plasmids Open
Metabolic engineering and synthetic biology are predicated on the precise control of gene expression. As synthetic biology expands beyond model organisms, more tools will be required that function robustly in a wide range of bacterial host…
View article: Tandem repeats in giant archaeal Borg elements undergo rapid evolution and create new intrinsically disordered regions in proteins
Tandem repeats in giant archaeal Borg elements undergo rapid evolution and create new intrinsically disordered regions in proteins Open
Borgs are huge, linear extrachromosomal elements associated with anaerobic methane-oxidizing archaea. Striking features of Borg genomes are pervasive tandem direct repeat (TR) regions. Here, we present six new Borg genomes and investigate …
View article: The pGinger family of expression plasmids
The pGinger family of expression plasmids Open
The pGinger suite of expression plasmids comprises 43 plasmids that will enable precise constitutive and inducible gene expression in a wide range of gram-negative bacterial species. Constitutive vectors are composed of 16 synthetic consti…
View article: Perspectives for self-driving labs in synthetic biology
Perspectives for self-driving labs in synthetic biology Open
Self-driving labs (SDLs) combine fully automated experiments with artificial intelligence (AI) that decides the next set of experiments. Taken to their ultimate expression, SDLs could usher a new paradigm of scientific research, where the …
View article: Perspectives for self-driving labs in synthetic biology
Perspectives for self-driving labs in synthetic biology Open
Self-driving labs (SDLs) combine fully automated experiments with artificial intelligence (AI) that decides the next set of experiments. Taken to their ultimate expression, SDLs could usher a new paradigm of scientific research, where the …
View article: Borg tandem repeats undergo rapid evolution and are under strong selection to create new intrinsically disordered regions in proteins
Borg tandem repeats undergo rapid evolution and are under strong selection to create new intrinsically disordered regions in proteins Open
Borgs are huge, linear extrachromosomal elements associated with anaerobic methane-oxidizing archaea. Striking features of Borg genomes are pervasive tandem direct repeat (TR) regions. Here, we present six new Borg genomes and investigate …
View article: Publisher Correction: Modular and tunable biological feedback control using a de novo protein switch
Publisher Correction: Modular and tunable biological feedback control using a de novo protein switch Open