Lucien E. Weiss
YOU?
Author Swipe
View article: Sticking to Green: Sustainable Solutions for Next-Generation Microelectronic Packaging with Plant-Derived and Bacterial Adhesives
Sticking to Green: Sustainable Solutions for Next-Generation Microelectronic Packaging with Plant-Derived and Bacterial Adhesives Open
View article: α-Synuclein driven cell susceptibility in Parkinson’s disease
α-Synuclein driven cell susceptibility in Parkinson’s disease Open
Early cellular events in Parkinson’s disease (PD) remain elusive. While aggregation of α-synuclein (αSyn) into Lewy bodies marks advanced pathology, smaller αSyn oligomers have been implicated in prodromal stages. Here we map αSyn oligomer…
View article: Author Correction: THY1-mediated mechanisms converge to drive YAP activation in skin homeostasis and repair
Author Correction: THY1-mediated mechanisms converge to drive YAP activation in skin homeostasis and repair Open
View article: Large-scale visualization of α-synuclein oligomers in Parkinson’s disease brain tissue
Large-scale visualization of α-synuclein oligomers in Parkinson’s disease brain tissue Open
Parkinson’s disease (PD) is a neurodegenerative condition characterized by the presence of intraneuronal aggregates containing fibrillar ɑ-synuclein known as Lewy bodies. These large end-stage species are formed by smaller soluble protein …
View article: Detecting directed motion and confinement in single-particle trajectories using hidden variables
Detecting directed motion and confinement in single-particle trajectories using hidden variables Open
Single-particle tracking is a powerful tool for understanding protein dynamics and characterizing microenvironments. As the motion of unconstrained nanoscale particles is governed by Brownian diffusion, deviations from this behavior are bi…
View article: Author response: Detecting directed motion and confinement in single-particle trajectories using hidden variables
Author response: Detecting directed motion and confinement in single-particle trajectories using hidden variables Open
View article: High-throughput DNA repair monitoring in Saccharomyces cerevisiae suggests SSB- and DSB-induced chromatin reconfiguration
High-throughput DNA repair monitoring in Saccharomyces cerevisiae suggests SSB- and DSB-induced chromatin reconfiguration Open
DNA repair is critical for cellular function and genomic stability across organisms. Yeast mating-type switching serves as an established model for studying DNA break repair and chromosome dynamics. However, real-time tracking of mating-ty…
View article: α-Synuclein driven cell susceptibility in Parkinson’s disease
α-Synuclein driven cell susceptibility in Parkinson’s disease Open
Early cellular events in Parkinson’s disease (PD) remain elusive. While aggregation of α-synuclein (αSyn) into Lewy bodies marks advanced pathology, smaller αSyn oligomers have been implicated in prodromal stages. Here we map αSyn oligomer…
View article: Condition-dependent, amorphous protein agglomerates control cytoplasmic rheology
Condition-dependent, amorphous protein agglomerates control cytoplasmic rheology Open
Summary Molecular crowding in the bacterial cytoplasm restricts the diffusion of large molecules, impacting cellular processes. However, how nutrient availability influences cytoplasmic rheology is not well understood. With single-particle…
View article: Patch deconvolution for Fourier light-field microscopy
Patch deconvolution for Fourier light-field microscopy Open
Imaging flow cytometry using Fourier light-field microscopy enables high-throughput three-dimensional cellular imaging, capable of capturing thousands of events per second. However, volumetric reconstruction speed remains orders of magnitu…
View article: Vortex light field microscopy: 3D spectral single-molecule imaging with a twist
Vortex light field microscopy: 3D spectral single-molecule imaging with a twist Open
3D single-molecule imaging reveals nanoscale structures in cell volumes but is limited by the need for spectrally distinct fluorophores. We address this limitation with vortex light field microscopy (VLFM), a 3D spectral single-molecule lo…
View article: Nuclear basket proteins regulate the distribution and mobility of nuclear pore complexes in budding yeast
Nuclear basket proteins regulate the distribution and mobility of nuclear pore complexes in budding yeast Open
Nuclear pore complexes (NPCs) mediate all traffic between the nucleus and the cytoplasm and are among the most stable protein assemblies in cells. Budding yeast cells carry two variants of NPCs which differ in the presence or absence of th…
View article: Fast analytical method to integrate multivariate Gaussians over hidden variables
Fast analytical method to integrate multivariate Gaussians over hidden variables Open
View article: High-throughput DNA repair monitoring in <i>Saccharomyces cerevisiae</i> reveals SSB- and DSB-induced chromatin reconfiguration
High-throughput DNA repair monitoring in <i>Saccharomyces cerevisiae</i> reveals SSB- and DSB-induced chromatin reconfiguration Open
DNA repair is critical for cellular function and genomic stability across organisms. Yeast mating-type switching serves as an established model for studying DNA break repair and chromatin dynamics. However, real-time tracking of mating-typ…
View article: Detecting directed motion and confinement in single-particle trajectories using hidden variables
Detecting directed motion and confinement in single-particle trajectories using hidden variables Open
Single-particle tracking is a powerful tool for understanding protein dynamics and characterizing microenvironments. As the motion of unconstrained nanoscale particles is governed by Brownian diffusion, deviations from this behavior are bi…
View article: Detecting directed motion and confinement in single-particle trajectories using hidden variables
Detecting directed motion and confinement in single-particle trajectories using hidden variables Open
Single-particle tracking is a powerful tool for understanding protein dynamics and characterizing microenvironments. As the motion of unconstrained nanoscale particles is governed by Brownian diffusion, deviations from this behavior are bi…
View article: Detecting directed motion and confinement in single-particle trajectories using hidden variables
Detecting directed motion and confinement in single-particle trajectories using hidden variables Open
Single-particle tracking is a powerful tool for understanding protein dynamics and characterizing microenvironments. As the motion of unconstrained nanoscale particles is governed by Brownian diffusion, deviations from this behavior are bi…
View article: RASP: Optimal Single Puncta Detection in Complex Cellular Backgrounds
RASP: Optimal Single Puncta Detection in Complex Cellular Backgrounds Open
Super-resolution and single-molecule microscopy are increasingly applied to complex biological systems. A major challenge of this approach is that fluorescent puncta must be detected in the low signal, high noise, heterogeneous background …
View article: Large-scale visualisation of α-synuclein oligomers in Parkinson's disease brain tissue
Large-scale visualisation of α-synuclein oligomers in Parkinson's disease brain tissue Open
Parkinson's disease (PD) is a common neurodegenerative condition characterised by the presence in the brain of large intraneuronal aggregates, known as Lewy bodies and Lewy neurites, containing fibrillar α-synuclein. According to the amylo…
View article: RASP: Optimal single fluorescent puncta detection in complex cellular backgrounds
RASP: Optimal single fluorescent puncta detection in complex cellular backgrounds Open
Super-resolution and single-molecule microscopy are increasingly applied to complex biological systems. A major challenge of this approach is that fluorescent puncta must be detected in the low signal, high noise, heterogeneous background …
View article: Nuclear basket proteins regulate the distribution and mobility of nuclear pore complexes in budding yeast
Nuclear basket proteins regulate the distribution and mobility of nuclear pore complexes in budding yeast Open
Nuclear pore complexes (NPCs) mediate all traffic between the nucleus and the cytoplasm and are among the most stable protein assemblies in cells. Budding yeast cells carry two variants of NPCs which differ in the presence or absence of th…
View article: Single-molecule imaging in the primary cilium
Single-molecule imaging in the primary cilium Open
View article: Synthetic cells with self-activating optogenetic proteins communicate with natural cells
Synthetic cells with self-activating optogenetic proteins communicate with natural cells Open
Development of regulated cellular processes and signaling methods in synthetic cells is essential for their integration with living materials. Light is an attractive tool to achieve this, but the limited penetration depth into tissue of vi…
View article: Quantifying cell-cycle-dependent chromatin dynamics during interphase by live 3D tracking
Quantifying cell-cycle-dependent chromatin dynamics during interphase by live 3D tracking Open
The study of cell cycle progression and regulation is important to our understanding of fundamental biophysics, aging, and disease mechanisms. Local chromatin movements are generally considered to be constrained and relatively consistent d…
View article: High-Throughput Imaging of CRISPR- and Recombinant Adeno-Associated Virus–Induced DNA Damage Response in Human Hematopoietic Stem and Progenitor Cells
High-Throughput Imaging of CRISPR- and Recombinant Adeno-Associated Virus–Induced DNA Damage Response in Human Hematopoietic Stem and Progenitor Cells Open
CRISPR-Cas technology has revolutionized gene editing, but concerns remain due to its propensity for off-target interactions. This, combined with genotoxicity related to both CRISPR-Cas9-induced double-strand breaks and transgene delivery,…
View article: Multiplexed PSF Engineering for Three-Dimensional Multicolor Particle Tracking
Multiplexed PSF Engineering for Three-Dimensional Multicolor Particle Tracking Open
Three-dimensional spatiotemporal tracking of microscopic particles in multiple colors is a challenging optical imaging task. Existing approaches require a trade-off between photon efficiency, field of view, mechanical complexity, spectral …
View article: 3D printable diffractive optical elements by liquid immersion
3D printable diffractive optical elements by liquid immersion Open
View article: Microscopic scan-free surface profiling over extended axial ranges by point-spread-function engineering
Microscopic scan-free surface profiling over extended axial ranges by point-spread-function engineering Open
Illuminated points reveal 3D microscopic surface topography.
View article: Learning an optimal PSF-pair for ultra-dense 3D localization microscopy
Learning an optimal PSF-pair for ultra-dense 3D localization microscopy Open
A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…
View article: Publisher Correction: DeepSTORM3D: dense 3D localization microscopy and PSF design by deep learning
Publisher Correction: DeepSTORM3D: dense 3D localization microscopy and PSF design by deep learning Open