Ludger Johannes
YOU?
Author Swipe
View article: Spatial N-glycan rearrangement on α5β1 integrin nucleates galectin-3 oligomers to determine endocytic fate
Spatial N-glycan rearrangement on α5β1 integrin nucleates galectin-3 oligomers to determine endocytic fate Open
Membrane glycoproteins frequently adopt different conformations when altering between active and inactive states. Here, we discover a molecular switch that exploits dynamic spatial rearrangements of N-glycans during such conformational tra…
View article: Galectin-3 Mediated Endocytosis of the Orphan G-Protein-Coupled Receptor GPRC5A
Galectin-3 Mediated Endocytosis of the Orphan G-Protein-Coupled Receptor GPRC5A Open
Galectins, a family of glycan-binding proteins, play crucial roles in various cellular functions, acting at both intracellular and extracellular levels. Among them, Galectin-3 (Gal-3) stands out as a unique member, possessing an intrinsica…
View article: Galectin-3 Mediated Endocytosis of the Orphan G-Protein-Coupled Receptor GPRC5A
Galectin-3 Mediated Endocytosis of the Orphan G-Protein-Coupled Receptor GPRC5A Open
Galectins, a family of glycan-binding proteins, play crucial roles in various cellular functions, acting at both intracellular and extracellular levels. Among them, Galectin-3 (Gal-3) stands out as a unique member, possessing an intrinsica…
View article: Acidification on the plasma membrane
Acidification on the plasma membrane Open
The pH balance between extracellular and intracellular space is crucial for a multitude of cellular processes. Real-time observation of pH fluctuations in the range 4-9 in live cells and tissues in a sensitive, non-invasive manner has beco…
View article: PSL Chemical Biology Symposia: The Increasing Impact of Chemistry in Life Sciences
PSL Chemical Biology Symposia: The Increasing Impact of Chemistry in Life Sciences Open
This symposium is the 6th Paris Sciences & Lettres (PSL) Chemical Biology meeting (2015, 2016, 2019, 2023, 2024, 2025) being held at Institut Curie. This initiative originally started in 2013 at Institut de Chimie des Substances Naturelles…
View article: Next-generation small molecule inhibitors of clathrin function acutely inhibit endocytosis
Next-generation small molecule inhibitors of clathrin function acutely inhibit endocytosis Open
Clathrin-mediated endocytosis (CME) is the predominant endocytic pathway in eukaryotic cells and a major regulator of cell physiology as it facilitates the internalization of receptors, channels, and transporters and viral entry. The clath…
View article: Publisher Correction: Growth factor-triggered de-sialylation controls glycolipid-lectin-driven endocytosis
Publisher Correction: Growth factor-triggered de-sialylation controls glycolipid-lectin-driven endocytosis Open
View article: Clathrin-independent endocytosis and retrograde transport in cancer cells promote cytotoxic CD8 T cell activation
Clathrin-independent endocytosis and retrograde transport in cancer cells promote cytotoxic CD8 T cell activation Open
Endophilin A3-mediated clathrin-independent endocytosis (EndoA3-mediated CIE) mediates the internalization of immunoglobulin-like proteins, including key immune synapse components. Here, we identify ICAM1 as a novel EndoA3-dependent cargo,…
View article: Clathrin-independent endocytosis and retrograde transport in cancer cells promote cytotoxic CD8 T cell activation
Clathrin-independent endocytosis and retrograde transport in cancer cells promote cytotoxic CD8 T cell activation Open
Endophilin A3-mediated clathrin-independent endocytosis (EndoA3-mediated CIE) mediates the internalization of immunoglobulin-like proteins, including key immune synapse components. Here, we identify ICAM1 as a novel EndoA3-dependent cargo,…
View article: Differential predictive value of resident memory CD8 <sup>+</sup> T cell subpopulations in patients with non-small-cell lung cancer treated by immunotherapy
Differential predictive value of resident memory CD8 <sup>+</sup> T cell subpopulations in patients with non-small-cell lung cancer treated by immunotherapy Open
Background A high density of resident memory T cells (T RM ) in tumors correlates with improved clinical outcomes in immunotherapy-treated patients. In most clinical studies, T RM are defined by the CD103 marker. However, it is clearly est…
View article: Mammalian cell-based production of glycans, glycopeptides and glycomodules
Mammalian cell-based production of glycans, glycopeptides and glycomodules Open
Access to defined glycans and glycoconjugates is pivotal for discovery, dissection, and harnessing of a range of biological functions orchestrated by cellular glycosylation processes and the glycome. We previously employed genetic glycoeng…
View article: Clathrin-independent endocytosis and retrograde transport in cancer cells promote cytotoxic CD8 T cell activation
Clathrin-independent endocytosis and retrograde transport in cancer cells promote cytotoxic CD8 T cell activation Open
Endophilin A3-mediated clathrin-independent endocytosis (EndoA3-mediated CIE) mediates the internalization of immunoglobulin-like proteins, including key immune synapse components. Here, we identify ICAM1 as a novel EndoA3-dependent cargo,…
View article: Exploration into Galectin-3 Driven Endocytosis and Lattices
Exploration into Galectin-3 Driven Endocytosis and Lattices Open
Essentially all plasma membrane proteins are glycosylated, and their activity is regulated by tuning their cell surface dynamics. This is achieved by glycan-binding proteins of the galectin family that either retain glycoproteins within la…
View article: Cytokine profile of anti-spike CD4+T cells predicts humoral and CD8+T cell responses after anti-SARS-CoV-2 mRNA vaccination
Cytokine profile of anti-spike CD4+T cells predicts humoral and CD8+T cell responses after anti-SARS-CoV-2 mRNA vaccination Open
View article: Differential predictive value of resident memory CD8<sup>+</sup>T cell subpopulations in non-small-cell lung cancer patients treated by immunotherapy
Differential predictive value of resident memory CD8<sup>+</sup>T cell subpopulations in non-small-cell lung cancer patients treated by immunotherapy Open
A high density of resident memory T cells (T RM ) in tumors correlates with improved clinical outcomes in immunotherapy-treated patients. However, in preclinical models, only some subpopulations of T RM are associated with cancer vaccine e…
View article: SLC3A2 N-glycosylation and Golgi remodeling regulate SLC7A amino acid exchangers and stress mitigation
SLC3A2 N-glycosylation and Golgi remodeling regulate SLC7A amino acid exchangers and stress mitigation Open
View article: Spatial N-glycan rearrangement on α<sub>5</sub>β<sub>1</sub>integrin nucleates galectin-3 oligomers to determine endocytic fate
Spatial N-glycan rearrangement on α<sub>5</sub>β<sub>1</sub>integrin nucleates galectin-3 oligomers to determine endocytic fate Open
Summary Membrane glycoproteins frequently adopt different conformations when altering between active and inactive states. Here, we discover a molecular switch that exploits dynamic spatial rearrangements of N-glycans during such conformati…
View article: Cytokine profile of anti-spike CD4+T cells predicts humoral and CD8+T cell responses after anti-SARS-CoV-2 mRNA vaccination
Cytokine profile of anti-spike CD4+T cells predicts humoral and CD8+T cell responses after anti-SARS-CoV-2 mRNA vaccination Open
Coordinating immune responses – humoral and cellular – is vital for protection against severe Covid-19. Our study evaluates a multicytokine CD4+T cell signature’s predictive potential for post-vaccinal serological and CD8+T cell responses.…
View article: Growth factor-induced desialylation for the fast control of endocytosis
Growth factor-induced desialylation for the fast control of endocytosis Open
Summary It is commonly assumed that the glycan makeup of glycoproteins that reach the cell surface is final and static. Here, we challenge this notion by the discovery of a molecular switch that induces acute and reversible changes of glyc…
View article: A synthetic delivery vector for mucosal vaccination
A synthetic delivery vector for mucosal vaccination Open
View article: CCDC 1046867: Experimental Crystal Structure Determination
CCDC 1046867: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: CCDC 1046866: Experimental Crystal Structure Determination
CCDC 1046866: Experimental Crystal Structure Determination Open
An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available …
View article: A synthetic delivery vector for mucosal vaccination
A synthetic delivery vector for mucosal vaccination Open
The success of mRNA-based vaccines during the Covid-19 pandemic has highlighted the value of this new platform for vaccine development against infectious disease. However, the CD8 + T cell response remains modest with mRNA vaccines, and th…
View article: Engineered Synthetic STxB for Enhanced Cytosolic Delivery
Engineered Synthetic STxB for Enhanced Cytosolic Delivery Open
Many molecular targets for cancer therapy are located in the cytosol. Therapeutic macromolecules are generally not able to spontaneously translocate across membranes to reach these cytosolic targets. Therefore a strong need exists for tool…
View article: Data from Tumor Targeting with Bacterial Shiga Toxin B Subunit in Genetic Porcine Models for Colorectal Cancer and Osteosarcoma
Data from Tumor Targeting with Bacterial Shiga Toxin B Subunit in Genetic Porcine Models for Colorectal Cancer and Osteosarcoma Open
The B subunit of bacterial Shiga toxin (STxB) is nontoxic and has low immunogenicity. Its receptor, the glycosphingolipid Gb3/CD77, is overexpressed on the cell surface of human colorectal cancer. We tested whether genetic porci…
View article: Supplementary Figure S3 from Synergy of Radiotherapy and a Cancer Vaccine for the Treatment of HPV-Associated Head and Neck Cancer
Supplementary Figure S3 from Synergy of Radiotherapy and a Cancer Vaccine for the Treatment of HPV-Associated Head and Neck Cancer Open
Combination treatment induces a long-term anti-metastatic immune response
View article: Supplementary Figure from Tumor Targeting with Bacterial Shiga Toxin B Subunit in Genetic Porcine Models for Colorectal Cancer and Osteosarcoma
Supplementary Figure from Tumor Targeting with Bacterial Shiga Toxin B Subunit in Genetic Porcine Models for Colorectal Cancer and Osteosarcoma Open
Supplementary Figure from Tumor Targeting with Bacterial Shiga Toxin B Subunit in Genetic Porcine Models for Colorectal Cancer and Osteosarcoma
View article: Data from Gastric Adenocarcinomas Express the Glycosphingolipid Gb<sub>3</sub>/CD77: Targeting of Gastric Cancer Cells with Shiga Toxin B-Subunit
Data from Gastric Adenocarcinomas Express the Glycosphingolipid Gb<sub>3</sub>/CD77: Targeting of Gastric Cancer Cells with Shiga Toxin B-Subunit Open
The B-subunit of the bacterial Shiga toxin (STxB), which is nontoxic and has low immunogenicity, can be used for tumor targeting of breast, colon, and pancreatic cancer. Here, we tested whether human gastric cancers, which are among the mo…
View article: Supplementary Methods, Figures 1-5, Tables 1-3 from Tumor-Specific Targeting of Pancreatic Cancer with Shiga Toxin B-Subunit
Supplementary Methods, Figures 1-5, Tables 1-3 from Tumor-Specific Targeting of Pancreatic Cancer with Shiga Toxin B-Subunit Open
PDF file - 313K
View article: Supplementary Figure 4 from Gastric Adenocarcinomas Express the Glycosphingolipid Gb<sub>3</sub>/CD77: Targeting of Gastric Cancer Cells with Shiga Toxin B-Subunit
Supplementary Figure 4 from Gastric Adenocarcinomas Express the Glycosphingolipid Gb<sub>3</sub>/CD77: Targeting of Gastric Cancer Cells with Shiga Toxin B-Subunit Open
STxB retains binding capacity to Gb3 at low pH in St3051 cells