Luis F. Schachner
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View article: Nucleosome context regulates chromatin reader preference
Nucleosome context regulates chromatin reader preference Open
Chromatin is more than a simple genome packaging system but rather locally distinguished by histone post-translational modifications (PTMs) that can directly change nucleosome structure and/or be “read” by chromatin-associated proteins to …
View article: Nucleosome context regulates chromatin reader preference
Nucleosome context regulates chromatin reader preference Open
Chromatin is more than a simple genome packaging system but rather locally distinguished by histone post-translational modifications (PTMs) that can directly change nucleosome structure and/or be “read” by chromatin-associated proteins to …
View article: Direct Readout of Multivalent Chromatin Reader-Nucleosome Interactions by Nucleosome Mass Spectrometry
Direct Readout of Multivalent Chromatin Reader-Nucleosome Interactions by Nucleosome Mass Spectrometry Open
Histone post-translational modifications (PTMs) often serve as distinct recognition sites for the recruitment of chromatin-associated proteins (CAPs) for epigenome regulation. While CAP-PTM interactions have been extensively studied using …
View article: Nucleosome context regulates chromatin reader preference
Nucleosome context regulates chromatin reader preference Open
Chromatin is more than a simple genome packaging system, and instead locally distinguished by histone post-translational modifications (PTMs) that can directly change nucleosome structure and / or be “read” by chromatin-associated proteins…
View article: Therapeutically targeting the classical complement pathway with antisense oligonucleotides in Alzheimer’s disease
Therapeutically targeting the classical complement pathway with antisense oligonucleotides in Alzheimer’s disease Open
The complement classical pathway (CP) is a key mediator of synapse loss and neurodegeneration in mouse models of Alzheimer’s (AD) and other neurodegenerative diseases. We analyzed human brain proteomics and found consistent elevations of a…
View article: Exposing the molecular heterogeneity of glycosylated biotherapeutics
Exposing the molecular heterogeneity of glycosylated biotherapeutics Open
View article: Exposing the molecular heterogeneity of glycosylated biotherapeutics
Exposing the molecular heterogeneity of glycosylated biotherapeutics Open
Glycosylated biotherapeutics are an emerging class of drugs with high molecular heterogeneity, which can affect their safety and efficacy. Characterizing this heterogeneity is crucial for drug development and quality assessment, but existi…
View article: Exposing the molecular heterogeneity of glycosylated biotherapeutics
Exposing the molecular heterogeneity of glycosylated biotherapeutics Open
Glycosylated biotherapeutics are an emerging class of drugs with high molecular heterogeneity, which can affect their safety and efficacy. Characterizing this heterogeneity is crucial for drug development and quality assessment, but existi…
View article: High-Throughput, Quantitative Analysis of Peptide-Exchanged MHCI Complexes by Native Mass Spectrometry
High-Throughput, Quantitative Analysis of Peptide-Exchanged MHCI Complexes by Native Mass Spectrometry Open
Immune monitoring in cancer immunotherapy involves screening CD8+ T-cell responses against neoantigens, the tumor-specific peptides presented by Major histocompatibility complex Class I (MHCI) on the cell surface. High-throughput immune mo…
View article: Revving an Engine of Human Metabolism: Activity Enhancement of Triosephosphate Isomerase via Hemi-Phosphorylation
Revving an Engine of Human Metabolism: Activity Enhancement of Triosephosphate Isomerase via Hemi-Phosphorylation Open
Triosephosphate isomerase (TPI) performs the 5th step in glycolysis, operates near the limit of diffusion, and is involved in "moonlighting" functions. Its dimer was found singly phosphorylated at Ser20 (pSer20) in human cells, with this p…
View article: Reassembling protein complexes after controlled disassembly by top-down mass spectrometry in native mode
Reassembling protein complexes after controlled disassembly by top-down mass spectrometry in native mode Open
View article: Separation and Characterization of Endogenous Nucleosomes by Native Capillary Zone Electrophoresis–Top-Down Mass Spectrometry
Separation and Characterization of Endogenous Nucleosomes by Native Capillary Zone Electrophoresis–Top-Down Mass Spectrometry Open
We report a novel platform [native capillary zone electrophoresis-top-down mass spectrometry (nCZE-TDMS)] for the separation and characterization of whole nucleosomes, their histone subunits, and post-translational modifications (PTMs). As…
View article: Decoding the protein composition of whole nucleosomes with Nuc-MS
Decoding the protein composition of whole nucleosomes with Nuc-MS Open
View article: Protocol for Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS: Sample Preparation, Data Acquisition and Analysis
Protocol for Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS: Sample Preparation, Data Acquisition and Analysis Open
The disassembly and digestion of nucleosome particles in current proteomics approaches forfeits correlations among histones and blurs nucleosome-level information. We developed Nuc-MS which analyzes whole nucleosomes and displays their his…
View article: Separation and Characterization of Endogenous Nucleosomes by Native Capillary Zone Electrophoresis – Top-Down Mass Spectrometry (nCZE-TDMS)
Separation and Characterization of Endogenous Nucleosomes by Native Capillary Zone Electrophoresis – Top-Down Mass Spectrometry (nCZE-TDMS) Open
We report a novel platform (native capillary zone electrophoresis – top-down mass spectrometry; nCZE-TDMS) for the separation and characterization of whole nucleosomes, their histone subunits, and PTMs. As the repeating unit of chromatin, …
View article: Development of novel methods for non-canonical myeloma protein analysis with an innovative adaptation of immunofixation electrophoresis, native top-down mass spectrometry, and middle-down <i>de novo</i> sequencing
Development of novel methods for non-canonical myeloma protein analysis with an innovative adaptation of immunofixation electrophoresis, native top-down mass spectrometry, and middle-down <i>de novo</i> sequencing Open
Objectives Multiple myeloma (MM) is a malignant plasma cell neoplasm, requiring the integration of clinical examination, laboratory and radiological investigations for diagnosis. Detection and isotypic identification of the monoclonal prot…
View article: Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS
Decoding the Protein Composition of Whole Nucleosomes with Nuc-MS Open
Nuc-MS characterizes histone modifications and variants directly from intact endogenous nucleosomes. Preserving whole nucleosome particles enables precise interrogation of their protein content, as for H3.3-containing nucleosomes which had…
View article: Using 10,000 Fragment Ions to Inform Scoring in Native Top-down Proteomics
Using 10,000 Fragment Ions to Inform Scoring in Native Top-down Proteomics Open
Protein fragmentation is a critical component of top-down proteomics, enabling gene-specific protein identification and full proteoform characterization. The factors that influence protein fragmentation include precursor charge, structure,…
View article: Individual Ion Mass Spectrometry Enhances the Sensitivity and Sequence Coverage of Top-Down Mass Spectrometry
Individual Ion Mass Spectrometry Enhances the Sensitivity and Sequence Coverage of Top-Down Mass Spectrometry Open
Charge detection mass spectrometry (CDMS) is mainly utilized to determine the mass of intact molecules. Previous applications of CDMS have determined the mass-to-charge ratio and the charge of large polymers, DNA molecules, and native prot…
View article: Native vs Denatured: An in Depth Investigation of Charge State and Isotope Distributions
Native vs Denatured: An in Depth Investigation of Charge State and Isotope Distributions Open
New tools and techniques have dramatically accelerated the field of structural biology over the past several decades. One potent and relatively new technique that is now being utilized by an increasing number of laboratories is the combina…
View article: Best practices and benchmarks for intact protein analysis for top-down mass spectrometry
Best practices and benchmarks for intact protein analysis for top-down mass spectrometry Open
View article: Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase
Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase Open
View article: Standard Proteoforms and Their Complexes for Native Mass Spectrometry
Standard Proteoforms and Their Complexes for Native Mass Spectrometry Open
Native mass spectrometry (nMS) is a technique growing at the interface of analytical chemistry, structural biology, and proteomics that enables the detection and partial characterization of non-covalent protein assemblies. Currently, the s…
View article: A novel crosslinking protocol stabilizes amyloid β oligomers capable of inducing Alzheimer's‐associated pathologies
A novel crosslinking protocol stabilizes amyloid β oligomers capable of inducing Alzheimer's‐associated pathologies Open
Amyloid β oligomers (AβOs) accumulate early in Alzheimer's disease ( AD ) and experimentally cause memory dysfunction and the major pathologies associated with AD , for example, tau abnormalities, synapse loss, oxidative damage, and cognit…
View article: Top-down characterization of endogenous protein complexes with native proteomics
Top-down characterization of endogenous protein complexes with native proteomics Open
View article: Top-down proteomics: Where we are, where we are going?
Top-down proteomics: Where we are, where we are going? Open