Luisa Massai
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View article: Characterization of a Luminescence-Based Serum Bactericidal Activity Assay for Human Sera Against a Panel of Salmonella Strains
Characterization of a Luminescence-Based Serum Bactericidal Activity Assay for Human Sera Against a Panel of Salmonella Strains Open
Salmonellosis remains a major cause of morbidity and mortality in low- and middle-income countries, despite the availability of effective vaccines against Salmonella enterica serovar Typhi (S. Typhi). In response, substantial efforts have …
View article: Development and Characterization of a Ten-Plex Assay to Measure Klebsiella pneumoniae Antigen-Specific IgG in Human Sera
Development and Characterization of a Ten-Plex Assay to Measure Klebsiella pneumoniae Antigen-Specific IgG in Human Sera Open
Klebsiella pneumoniae is a leading cause of nosocomial infections, neonatal sepsis, and childhood mortality worldwide. A drastic rise in antibiotic-resistant isolates poses an urgent threat to humanity, and the World Health Organization (W…
View article: Early life serological profiles and the development of natural protective humoral immunity to<i>Streptococcus pyogenes</i>in a high burden setting
Early life serological profiles and the development of natural protective humoral immunity to<i>Streptococcus pyogenes</i>in a high burden setting Open
Streptococcus pyogenes leads to 500,000 deaths annually; many due to rheumatic heart disease in low-income settings. Limited understanding of natural protective immunity to S. pyogenes hinders vaccine development. We describe the evolution…
View article: The Salmonella Paratyphi A O-Antigen Glycoconjugate Vaccine Is Able to Induce Antibodies with Bactericidal Activity Against a Panel of Clinical Isolates
The Salmonella Paratyphi A O-Antigen Glycoconjugate Vaccine Is Able to Induce Antibodies with Bactericidal Activity Against a Panel of Clinical Isolates Open
Background: Typhoid and paratyphoid fevers represent a global health burden, especially in Southern Asia, exacerbated by the increase in antimicrobial resistance. While vaccines against Salmonella Typhi have been successfully introduced, a…
View article: Characterization of an IL-8 cleavage inhibition assay to determine the functionality of anti-SpyCEP antibodies in human sera
Characterization of an IL-8 cleavage inhibition assay to determine the functionality of anti-SpyCEP antibodies in human sera Open
Exposure to Group A Streptococcus leads to a broad spectrum of disease and sequelae, as the bacterium employs a wide range of virulence factors to facilitate colonization of the host, propagation and onward transmission, disrupting both in…
View article: Design of a Glycoconjugate Vaccine Against Salmonella Paratyphi A
Design of a Glycoconjugate Vaccine Against Salmonella Paratyphi A Open
Background/Objectives: Typhoid and paratyphoid fever together are responsible for millions of cases and thousands of deaths per year, most of which occur in children in South and Southeast Asia. While typhoid conjugate vaccines (TCVs) are …
View article: Development and characterization of high-throughput serological assays to measure magnitude and functional immune response against S. Paratyphi A in human samples
Development and characterization of high-throughput serological assays to measure magnitude and functional immune response against S. Paratyphi A in human samples Open
Typhoid and Paratyphoid fever cause a global health burden, especially for the children of Southern Asia. The impact of the disease is further exacerbated by the dramatic increase of antimicrobial resistance. While vaccines against Salmone…
View article: Adjuvanted Modified Bacterial Antigens for Single-Dose Vaccines
Adjuvanted Modified Bacterial Antigens for Single-Dose Vaccines Open
Alum is the most used vaccine adjuvant, due to its safety, low cost and adjuvanticity to various antigens. However, the mechanism of action of alum is complex and not yet fully understood, and the immune responses elicited can be weak and …
View article: Qualification of an enzyme-linked immunosorbent assay for quantification of anti-Vi IgG in human sera
Qualification of an enzyme-linked immunosorbent assay for quantification of anti-Vi IgG in human sera Open
Effective vaccines against Salmonella Typhi, targeting the Vi capsular polysaccharide, have been developed and are being introduced into routine immunization in endemic countries. Vi conjugated vaccines are also being tested in new multi-c…
View article: O-Antigen decorations in Salmonella enterica play a key role in eliciting functional immune responses against heterologous serovars in animal models
O-Antigen decorations in Salmonella enterica play a key role in eliciting functional immune responses against heterologous serovars in animal models Open
Introduction Different serovars of Salmonella enterica cause systemic diseases in humans including enteric fever, caused by S. Typhi and S. Paratyphi A, and invasive nontyphoidal salmonellosis (iNTS), caused mainly by S. Typhimurium and S.…
View article: Testing a Recombinant Form of Tetanus Toxoid as a Carrier Protein for Glycoconjugate Vaccines
Testing a Recombinant Form of Tetanus Toxoid as a Carrier Protein for Glycoconjugate Vaccines Open
Glycoconjugate vaccines play a major role in the prevention of infectious diseases worldwide, with significant impact on global health, enabling the polysaccharides to induce immunogenicity in infants and immunological memory. Tetanus toxo…
View article: GMMA as an Alternative Carrier for a Glycoconjugate Vaccine against Group A Streptococcus
GMMA as an Alternative Carrier for a Glycoconjugate Vaccine against Group A Streptococcus Open
Group A Streptococcus (GAS) causes about 500,000 annual deaths globally, and no vaccines are currently available. The Group A Carbohydrate (GAC), conserved across all GAS serotypes, conjugated to an appropriate carrier protein, represents …
View article: Development and Characterisation of a Four-Plex Assay to Measure Streptococcus pyogenes Antigen-Specific IgG in Human Sera
Development and Characterisation of a Four-Plex Assay to Measure Streptococcus pyogenes Antigen-Specific IgG in Human Sera Open
The measurement of antibodies to vaccine antigens is crucial for research towards a safe and effective vaccine for Streptococcus pyogenes (Strep A). We describe the establishment and detailed characterisation of a four-plex assay to measur…
View article: Impact of O-Acetylation on S. flexneri 1b and 2a O-Antigen Immunogenicity in Mice
Impact of O-Acetylation on S. flexneri 1b and 2a O-Antigen Immunogenicity in Mice Open
Shigellosis is a diarrheal disease caused prevalently by Shigella flexneri and S. sonnei and representing a major global health risk, particularly in developing countries. Bacterial O-antigen (OAg) is the primary target of the host immune …