Lukas Bernauer
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View article: Utilizing native nanodiscs to isolate active TRPC3 channels and expand structural analysis capabilities
Utilizing native nanodiscs to isolate active TRPC3 channels and expand structural analysis capabilities Open
Recent advances in structural biology have provided insights into TRPC3, a TRP family member involved in various (patho)physiological processes. However, the lack of structural information on the channel’s open pore hampers understanding o…
View article: Human Sterols Are Overproduced, Stored and Excreted in Yeasts
Human Sterols Are Overproduced, Stored and Excreted in Yeasts Open
Sterols exert a profound influence on numerous cellular processes, playing a crucial role in both health and disease. However, comprehending the effects of sterol dysfunction on cellular physiology is challenging. Consequently, numerous pr…
View article: Sterol interactions influence the function of Wsc sensors
Sterol interactions influence the function of Wsc sensors Open
The Wsc1, Wsc2, and Wsc3 proteins are essential cell surface sensors that respond to cell wall perturbation by activating the cell wall integrity pathway (CWIP). We show here that in situ production of cholesterol (in place of ergosterol) …
View article: Applying the auxin-based degron system for the inducible, reversible and complete protein degradation in Komagataella phaffii
Applying the auxin-based degron system for the inducible, reversible and complete protein degradation in Komagataella phaffii Open
The auxin-inducible degron (AID) system is a useful technique to rapidly deplete any protein of interest "on-demand." In this study, we successfully established the AID system for the "biotech" yeast Komagataella phaffii. First, we …
View article: Komagataella phaffii as Emerging Model Organism in Fundamental Research
Komagataella phaffii as Emerging Model Organism in Fundamental Research Open
Komagataella phaffii ( Pichia pastoris ) is one of the most extensively applied yeast species in pharmaceutical and biotechnological industries, and, therefore, also called the biotech yeast. However, thanks to more advanced strain enginee…
View article: 'Applying the Auxin-Based Degron System for the Inducible, Reversible and Complete Protein Degradation in <i>Komagataella phaffii</i>'
'Applying the Auxin-Based Degron System for the Inducible, Reversible and Complete Protein Degradation in <i>Komagataella phaffii</i>' Open