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View article: Increased purity and refolding yield of bacterial inclusion bodies by recursive high pressure homogenization
Increased purity and refolding yield of bacterial inclusion bodies by recursive high pressure homogenization Open
Therapeutic proteins are often produced intracellularly in the bacterium Escherichia coli in aggregated form, known as inclusion bodies, which must be solubilized and refolded to recover the biologically active product. However, the indust…
View article: Morphological and physiological characterization of filamentous Lentzea aerocolonigenes: Comparison of biopellets by microscopy and flow cytometry
Morphological and physiological characterization of filamentous Lentzea aerocolonigenes: Comparison of biopellets by microscopy and flow cytometry Open
Cell morphology of filamentous microorganisms is highly interesting during cultivations as it is often linked to productivity and can be influenced by process conditions. Hence, the characterization of cell morphology is of major importanc…
View article: A Chemometric Tool to Monitor and Predict Cell Viability in Filamentous Fungi Bioprocesses Using UV Chromatogram Fingerprints
A Chemometric Tool to Monitor and Predict Cell Viability in Filamentous Fungi Bioprocesses Using UV Chromatogram Fingerprints Open
Monitoring process variables in bioprocesses with complex expression systems, such as filamentous fungi, requires a vast number of offline methods or sophisticated inline sensors. In this respect, cell viability is a crucial process variab…
View article: The filamentous fungus Penicillium chrysogenum analysed via flow cytometry—a fast and statistically sound insight into morphology and viability
The filamentous fungus Penicillium chrysogenum analysed via flow cytometry—a fast and statistically sound insight into morphology and viability Open
Filamentous fungi serve as production host for a number of highly relevant biotechnological products, like penicillin. In submerged culture, morphology can be exceptionally diverse and is influenced by several process parameters, like aera…
View article: The filamentous fungal pellet—relationship between morphology and productivity
The filamentous fungal pellet—relationship between morphology and productivity Open
Filamentous fungi are used for the production of a multitude of highly relevant biotechnological products like citric acid and penicillin. In submerged culture, fungi can either grow in dispersed form or as spherical pellets consisting of …