Łukasz Krych
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View article: Enhancing the sensory and nutritional properties of faba bean protein through fermentation with lactic acid bacteria and Bacillus spp
Enhancing the sensory and nutritional properties of faba bean protein through fermentation with lactic acid bacteria and Bacillus spp Open
Faba beans are rich in protein and fiber, but their consumption is limited by off-flavors, and antinutritional compounds. This study explored fermentations with lactic acid bacteria (LAB) and Bacillus spp. as a strategy to improve the sens…
View article: Pig meniscus single-cell sequencing reveals highly active red zone chondrocyte populations involved in stemness maintenance and vascularization development
Pig meniscus single-cell sequencing reveals highly active red zone chondrocyte populations involved in stemness maintenance and vascularization development Open
Meniscus injuries are widespread and the available treatments do not offer enough healing potential. Here, we provide critical support for using pigs as a biological model for meniscal degeneration and the development of cutting-edge thera…
View article: <i>De novo</i> clustering of long-read amplicons improves phylogenetic insight into microbiome data
<i>De novo</i> clustering of long-read amplicons improves phylogenetic insight into microbiome data Open
Long-read amplicon profiling through read classification limits phylogenetic analysis of amplicons while community analysis of multicopy genes, relying on unique molecular identifier (UMI) corrections, often demands deep sequencing. To add…
View article: Investigating fecal microbiota transplants from individuals with anorexia nervosa in antibiotic-treated mice using a cross-over study design
Investigating fecal microbiota transplants from individuals with anorexia nervosa in antibiotic-treated mice using a cross-over study design Open
View article: Metagenomic analysis of 130 years old Danish starter culture material including sequence analysis of the genome of a Lactococcus cremoris starter
Metagenomic analysis of 130 years old Danish starter culture material including sequence analysis of the genome of a Lactococcus cremoris starter Open
View article: Characterization of a virulent bacteriophage consortium targeting Enterobacteriaceae from inflamed preterm gut mucosa
Characterization of a virulent bacteriophage consortium targeting Enterobacteriaceae from inflamed preterm gut mucosa Open
Preterm infants have a high risk of intestinal inflammation which can progress to necrotizing enterocolitis (NEC). The gut microbial colonization commencing at birth is essential for proper intestinal maturation, but this process is often …
View article: Enhancing the Sensory and Nutritional Properties of Faba Bean Protein Through Fermentation with Lactic Acid Bacteria and Bacillus Spp
Enhancing the Sensory and Nutritional Properties of Faba Bean Protein Through Fermentation with Lactic Acid Bacteria and Bacillus Spp Open
View article: Wild-Mouse-Derived Gut Microbiome Transplantation in Laboratory Mice Partly Alleviates House-Dust-Mite-Induced Allergic Airway Inflammation
Wild-Mouse-Derived Gut Microbiome Transplantation in Laboratory Mice Partly Alleviates House-Dust-Mite-Induced Allergic Airway Inflammation Open
Laboratory mice are instrumental for preclinical research but there are serious concerns that the use of a clean standardized environment for specific-pathogen-free (SPF) mice results in poor bench-to-bedside translation due to their immat…
View article: Matching excellence: Oxford Nanopore Technologies’ rise to parity with Pacific Biosciences in genome reconstruction of non-model bacterium with high G+C content
Matching excellence: Oxford Nanopore Technologies’ rise to parity with Pacific Biosciences in genome reconstruction of non-model bacterium with high G+C content Open
The reconstruction of complete bacterial genomes is essential for microbial research, offering insights into genetic content, ontology and regulation. While Pacific Biosciences (PacBio) provides high-quality genomes, its cost remains a lim…
View article: Gut microbiota in rainbow trout Oncorhynchus mykiss with different susceptibility to Flavobacterium psychrophilum infection
Gut microbiota in rainbow trout Oncorhynchus mykiss with different susceptibility to Flavobacterium psychrophilum infection Open
View article: A single-stranded based library preparation method for virome characterization
A single-stranded based library preparation method for virome characterization Open
Background The gut virome is an integral component of the gut microbiome, playing a crucial role in maintaining gut health. However, accurately depicting the entire gut virome is challenging due to the inherent diversity of genome types (d…
View article: Editorial: Preparation, function and application of postbiotics
Editorial: Preparation, function and application of postbiotics Open
View article: Liraglutide and Colesevelam Change Serum and Fecal Bile Acid Levels in a Randomized Trial With Patients With Bile Acid Diarrhea
Liraglutide and Colesevelam Change Serum and Fecal Bile Acid Levels in a Randomized Trial With Patients With Bile Acid Diarrhea Open
INTRODUCTION: Both liraglutide and colesevelam improve bile acid diarrhea symptoms. Colesevelam binds excess amounts of diarrhea-causing bile acids in the colon, whereas the mode of action for liraglutide remains elusive. In this article, …
View article: Gut microbiota patterns associated with duration of diarrhea in children under five years of age in Ethiopia
Gut microbiota patterns associated with duration of diarrhea in children under five years of age in Ethiopia Open
Diarrhea claims >500,000 lives annually among children under five years of age in low- and middle-income countries. Mortality due to acute diarrhea (<7 days' duration) is decreasing, but prolonged (7-13 days) and persistent (≥14 days of du…
View article: Low‐gainer diet‐induced obese microbiota transplanted mice exhibit increased fighting
Low‐gainer diet‐induced obese microbiota transplanted mice exhibit increased fighting Open
Weight gain variation is a great challenge in diet‐induced obesity studies since low‐gainer animals are of limited experimental value. The inbred C57BL/6 (B6) mice are frequently used models due to their genetic homogeneity and susceptibil…
View article: Draft genome sequences of 53 <i>Lactococcus</i> and <i>Leuconostoc</i> strains isolated from two undefined DL-starter cultures
Draft genome sequences of 53 <i>Lactococcus</i> and <i>Leuconostoc</i> strains isolated from two undefined DL-starter cultures Open
This study presents the complete genomes of 53 strains of Lactococcus and Leuconostoc isolated from two undefined DL-starter cultures originating from Denmark, Tistrup, and P. The genomes were reconstructed using long-read, nanopore-based …
View article: A single strand-based library preparation method for unbiased virome characterization
A single strand-based library preparation method for unbiased virome characterization Open
Background The gut virome is an integral component of the gut microbiome, playing a crucial role in maintaining gut health. However, accurately depicting the entire gut virome is challenging due to the inherent diversity of genome types (d…
View article: Randomised, Placebo-Controlled Investigation of the Impact of Probiotic Consumption on Gut Microbiota Diversity and the Faecal Metabolome in Seniors
Randomised, Placebo-Controlled Investigation of the Impact of Probiotic Consumption on Gut Microbiota Diversity and the Faecal Metabolome in Seniors Open
Aging has been associated with a changed composition and function of the gut microbiota (GM). Here, we investigate the effects of the multi-strain probiotic HOWARU® Restore on GM composition and function in seniors. Ninety-eight healthy ad…
View article: A single strand-based library preparation method for unbiased virome characterization
A single strand-based library preparation method for unbiased virome characterization Open
The gut virome is an integral component of the gut microbiome, playing a crucial role in maintaining gut health. However, accurately depicting the entire gut virome is challenging due to the inherent limitations and biases associated with …
View article: Matching Excellence: ONT’s Rise to Parity with PacBio in Genome Reconstruction of Non-Model Bacterium with High GC Content
Matching Excellence: ONT’s Rise to Parity with PacBio in Genome Reconstruction of Non-Model Bacterium with High GC Content Open
Reconstruction of complete bacterial genomes is a vital aspect of microbial research, as it provides complex information about genetic content, gene ontology, and regulation. It has become a domain of 3rd generation, long-read sequencing p…
View article: Delayed Gut Colonization Changes Future Insulin Resistance and Hepatic Gene Expression but Not Adiposity in Obese Mice
Delayed Gut Colonization Changes Future Insulin Resistance and Hepatic Gene Expression but Not Adiposity in Obese Mice Open
Objective . The importance of early microbial dysbiosis in later development of obesity and metabolic disorders has been a subject of debate. Here we tested cause and effect in mice. Methods . Germ‐free male Swiss Webster mice were coloniz…
View article: Metagenomic Analysis of 130 Years Old Danish Starter Culture Material Including Sequence Analysis of the Genome of a Lactococcus Cremoris Starter
Metagenomic Analysis of 130 Years Old Danish Starter Culture Material Including Sequence Analysis of the Genome of a Lactococcus Cremoris Starter Open
View article: Dietary seaweed (Saccharina latissima) supplementation in pigs induces localized immunomodulatory effects and minor gut microbiota changes during intestinal helminth infection
Dietary seaweed (Saccharina latissima) supplementation in pigs induces localized immunomodulatory effects and minor gut microbiota changes during intestinal helminth infection Open
Brown seaweeds have a rich bioactive content known to modulate biological processes, including the mucosal immune response and microbiota function, and may therefore have the potential to control enteric pathogens. Here, we tested if dieta…
View article: <i>De novo</i>clustering of long-read amplicons improves phylogenetic insight into microbiome data
<i>De novo</i>clustering of long-read amplicons improves phylogenetic insight into microbiome data Open
Long-read amplicon profiling through read classification limits phylogenetic analysis of amplicons while community analysis of multicopy genes, relying on unique molecular identifier (UMI) corrections, often demands deep sequencing. To add…
View article: Cesarean section induced dysbiosis promotes type 2 immunity but not oxazolone-induced dermatitis in mice
Cesarean section induced dysbiosis promotes type 2 immunity but not oxazolone-induced dermatitis in mice Open
Delivery by cesarean section (CS) is associated with an altered gut microbiota (GM) colonization and a higher risk of later chronic inflammatory diseases. Studies investigating the association between CS and atopic dermatitis (AD) are cont…
View article: Investigating the Rescue Potential of Fecal Microbiota Transplants in Anorexia Nervosa using Antibiotic-treated Mice in a Split Group Cross-Over Study
Investigating the Rescue Potential of Fecal Microbiota Transplants in Anorexia Nervosa using Antibiotic-treated Mice in a Split Group Cross-Over Study Open
Anorexia nervosa (AN) is a complex and serious mental disorder, which may affect individuals of all ages and sex, but primarily affecting young women. The disease is characterized by a disturbed body image, restrictive eating behavior, and…
View article: Investigating the Rescue Potential of Fecal Microbiota Transplants in Anorexia Nervosa using Antibiotic-treated Mice in a Split Group Cross-Over Study
Investigating the Rescue Potential of Fecal Microbiota Transplants in Anorexia Nervosa using Antibiotic-treated Mice in a Split Group Cross-Over Study Open
Anorexia nervosa (AN) is a complex and serious mental disorder, which may affect individuals of all ages and sex, but primarily affecting young women. The disease is characterized by a disturbed body image, restrictive eating behavior, and…
View article: Dietary non-starch polysaccharides impair immunity to enteric nematode infection
Dietary non-starch polysaccharides impair immunity to enteric nematode infection Open
View article: Used Nasogastric Feeding Tubes from Neonates Contain Infant-Specific Bacterial Profiles
Used Nasogastric Feeding Tubes from Neonates Contain Infant-Specific Bacterial Profiles Open
Nasogastric feeding tubes (NG-tubes) from neonates contain potentially pathogenic bacteria. Using culture-based techniques, we have previously determined that the usage duration of NG-tubes did not impact the colonization of the nasogastri…
View article: Maternal antibiotic exposure increases total islet number and volume in the neonatal pancreas and reduces HbA1c and insulitis in adult female non‐obese diabetic mice
Maternal antibiotic exposure increases total islet number and volume in the neonatal pancreas and reduces HbA1c and insulitis in adult female non‐obese diabetic mice Open
Background Early antibiotic treatment changes susceptibility to disease in rodent models for type 1 diabetes, but the impact that gestational antibiotics may have on pancreatic embryogenesis and β cell function later in life is still unkno…