Bennett L. Ibey
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View article: Effect of nanosecond pulsed electric fields (nsPEFs) on coronavirus survival
Effect of nanosecond pulsed electric fields (nsPEFs) on coronavirus survival Open
Previous work demonstrated inactivation of influenza virus by GHz frequency electromagnetic fields. Despite theoretical and experimental results, the underlying mechanism driving this inactivation remains unknown. One hypothesis is that th…
View article: Control of the Electroporation Efficiency of Nanosecond Pulses by Swinging the Electric Field Vector Direction
Control of the Electroporation Efficiency of Nanosecond Pulses by Swinging the Electric Field Vector Direction Open
Reversing the pulse polarity, i.e., changing the electric field direction by 180°, inhibits electroporation and electrostimulation by nanosecond electric pulses (nsEPs). This feature, known as “bipolar cancellation,” enables selective remo…
View article: Evaluation of inactivation of bovine coronavirus by low-level radiofrequency irradiation
Evaluation of inactivation of bovine coronavirus by low-level radiofrequency irradiation Open
Inactivation of influenza A virus by radiofrequency (RF) energy exposure at levels near Institute of Electrical and Electronics Engineers (IEEE) safety thresholds has been reported. The authors hypothesized that this inactivation was throu…
View article: Rapid and precise tracking of water influx and efflux across cell membranes induced by a pulsed electric field
Rapid and precise tracking of water influx and efflux across cell membranes induced by a pulsed electric field Open
Quantitative measurements of water content within a single cell are notoriously difficult. In this work, we introduce a single-shot optical method for tracking the intracellular water content, by mass and volume, of a single cell at video …
View article: Pulsed Electric Field Ablation of Esophageal Malignancies and Mitigating Damage to Smooth Muscle: An In Vitro Study
Pulsed Electric Field Ablation of Esophageal Malignancies and Mitigating Damage to Smooth Muscle: An In Vitro Study Open
Cancer ablation therapies aim to be efficient while minimizing damage to healthy tissues. Nanosecond pulsed electric field (nsPEF) is a promising ablation modality because of its selectivity against certain cell types and reduced neuromusc…
View article: Evaluation of Viral Inactivation on Dry Surface by High Peak Power Microwave (HPPM) Exposure
Evaluation of Viral Inactivation on Dry Surface by High Peak Power Microwave (HPPM) Exposure Open
Previous research has shown that virus infectivity can be dramatically reduced by radio frequency exposure in the gigahertz (GHz) frequency range. Given the worldwide SARS‐CoV‐2 pandemic, which has caused over 1 million deaths and has had …
View article: Inside Front Cover
Inside Front Cover Open
We introduce quantitative phase imaging (QPI) as a useful tool for the study of single cells undergoing cytoskeletal remodeling after nanosecond pulsed electric field (nsPEF) exposure. In particular, we use cell swelling, dry mass and diso…
View article: Changes in the Excitability of Primary Hippocampal Neurons Following Exposure to 3.0 GHz Radiofrequency Electromagnetic Fields
Changes in the Excitability of Primary Hippocampal Neurons Following Exposure to 3.0 GHz Radiofrequency Electromagnetic Fields Open
Exposures to low intensity radiofrequency electromagnetic fields (RF-EMFs, 100 kHz to 6 GHz) have been associated with both positive and negative effects on cognitive behavior. To elucidate the mechanism of RF-EMF interaction, a few studie…
View article: Quantitative phase microscopy monitors subcellular dynamics in single cells exposed to nanosecond pulsed electric fields
Quantitative phase microscopy monitors subcellular dynamics in single cells exposed to nanosecond pulsed electric fields Open
A substantial body of literature exists to study the dynamics of single cells exposed to short duration (<1 μs), high peak power (~1 MV/m) transient electric fields. Much of this research is limited to traditional fluorescence‐based micros…
View article: Visualizing bleb mass dynamics in single cells using quantitative phase microscopy
Visualizing bleb mass dynamics in single cells using quantitative phase microscopy Open
Understanding biological responses to directed energy (DE) is critical to ensure the safety of personnel within the Department of Defense. At the Air Force Research Laboratory, we have developed or adapted advanced optical imaging systems …
View article: 600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus
600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus Open
Cell suspensions of Escherichia coli and Lactobacillus acidophilus were exposed to 600-ns pulsed electric fields (nsPEFs) at varying amplitudes (Low-13.5, Mid-18.5 or High-23.5 kV cm −1 ) and pulse numbers (0 (sham), 1, 5, 10, 100 or 1000)…
View article: Additional file 1 of 600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus
Additional file 1 of 600-ns pulsed electric fields affect inactivation and antibiotic susceptibilities of Escherichia coli and Lactobacillus acidophilus Open
Additional file 1: Table S1. Zones of growth inhibition for E. coli.
View article: Tracking Lysosome Migration within Chinese Hamster Ovary (CHO) Cells Following Exposure to Nanosecond Pulsed Electric Fields
Tracking Lysosome Migration within Chinese Hamster Ovary (CHO) Cells Following Exposure to Nanosecond Pulsed Electric Fields Open
Above a threshold electric field strength, 600 ns-duration pulsed electric field (nsPEF) exposure substantially porates and permeabilizes cellular plasma membranes in aqueous solution to many small ions. Repetitive exposures increase perme…
View article: Nanosecond pulsed electric field exposure does not induce the unfolded protein response in adult human dermal fibroblasts
Nanosecond pulsed electric field exposure does not induce the unfolded protein response in adult human dermal fibroblasts Open
Cell‐circuit models have suggested that nanosecond pulsed electric fields (nsPEFs) can disrupt intracellular membranes including endoplasmic reticulum (ER), mitochondria, and/or nucleus thereby inducing intrinsic apoptotic pathways. Theref…
View article: Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation
Asymmetrical bipolar nanosecond electric pulse widths modify bipolar cancellation Open
A bipolar (BP) nanosecond electric pulse (nsEP) exposure generates reduced calcium influx compared to a unipolar (UP) nsEP. This attenuated physiological response from a BP nsEP exposure is termed “ bipolar cancellation ” ( BPC ). The pred…
View article: Ryanodine and IP<sub>3</sub> receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation
Ryanodine and IP<sub>3</sub> receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation Open
Pulsed infrared (IR) laser energy has been shown to modulate neurological activity through both stimulation and inhibition of action potentials. While the mechanism(s) behind this phenomenon is (are) not completely understood, certain hypo…
View article: Probe beam deflection optical imaging of thermal and mechanical phenomena resulting from nanosecond electric pulse (nsEP) exposure in-vitro
Probe beam deflection optical imaging of thermal and mechanical phenomena resulting from nanosecond electric pulse (nsEP) exposure in-vitro Open
Electric-field induced physical phenomena, such as thermal, mechanical and electrochemical dynamics, may be the driving mechanism behind bioeffects observed in mammalian cells during exposure to nanosecond-duration electric pulses (nsEP) i…
View article: Adult human dermal fibroblasts exposed to nanosecond electrical pulses exhibit genetic biomarkers of mechanical stress
Adult human dermal fibroblasts exposed to nanosecond electrical pulses exhibit genetic biomarkers of mechanical stress Open
This work provides strong evidence that cells exposed to nsEP interpret the insult as a mechanical stress.
View article: Action potential block in neurons by infrared light
Action potential block in neurons by infrared light Open
Short infrared laser pulses (SILP) have many physiological effects on cells, including the ability to stimulate action potentials (APs) in neurons. Here, we show that SILPs can also reversibly block APs. Reversible AP block in hippocampal …
View article: Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP)
Evaluation of the Genetic Response of U937 and Jurkat Cells to 10-Nanosecond Electrical Pulses (nsEP) Open
Nanosecond electrical pulse (nsEP) exposure activates signaling pathways, produces oxidative stress, stimulates hormone secretion, causes cell swelling and induces apoptotic and necrotic death. The underlying biophysical connection(s) betw…