Luke S. Dunaway
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View article: CD47 mediates oxygen-responsive activation of RBC eNOS during circulatory transit
CD47 mediates oxygen-responsive activation of RBC eNOS during circulatory transit Open
BACKGROUND: Red blood cells (RBCs) contain active type 3 endothelial nitric oxide synthase (eNOS); however, the precise regulation of RBC eNOS during circulation remains unclear. Our previous work demonstrated that RBC eNOS activity oscill…
View article: Impact of Endothelial Diversity and Dysfunction on Cardiovascular Disease
Impact of Endothelial Diversity and Dysfunction on Cardiovascular Disease Open
Endothelial cells (ECs) are functionally heterogeneous, even in vascular beds within the same organ. As the key cell type lining the vascular lumen, ECs regulate vascular tone (control of blood vessel diameter), permeability of molecules, …
View article: Pannexins in the vasculature
Pannexins in the vasculature Open
Pannexins (PANX1, PANX2, PANX3) are a family of large-pore, ion and metabolite channels present throughout the blood and lymphatic vascular networks. PANX1 has near-ubiquitous expression in the cardiovascular system and is the most highly …
View article: Divergent roles of red cell arginase in humans and mice: RBC Arg1 KO mice show preserved systemic l-arginine bioavailability and infarct size in vivo
Divergent roles of red cell arginase in humans and mice: RBC Arg1 KO mice show preserved systemic l-arginine bioavailability and infarct size in vivo Open
View article: The Cells of the Vasculature: Advances in the Regulation of Vascular Tone in the Brain and Periphery
The Cells of the Vasculature: Advances in the Regulation of Vascular Tone in the Brain and Periphery Open
The vasculature is a complex tissue in which multiple cell types coordinate the regulation of tissue perfusion in response to hemodynamic and biochemical signals. Advances in this field are continuing to deepen our understanding of the rel…
View article: Time-restricted feeding reduces cardiovascular disease risk in obese mice
Time-restricted feeding reduces cardiovascular disease risk in obese mice Open
Disrupted feeding and fasting cycles as well as chronic high-fat diet-induced (HFD-induced) obesity are associated with cardiovascular disease risk factors. We designed studies that determined whether 2 weeks of time-restricted feeding (TR…
View article: Aryl Hydrocarbon Receptor Activation Promotes Effector CD4+ T Cell Homeostasis and Restrains Salt-Sensitive Hypertension
Aryl Hydrocarbon Receptor Activation Promotes Effector CD4+ T Cell Homeostasis and Restrains Salt-Sensitive Hypertension Open
Excess dietary salt and salt-sensitivity contribute to cardiovascular disease. Distinct T cell phenotypic responses to high salt and hypertension, as well as influences from environmental cues, are not well understood. The aryl hydrocarbon…
View article: Generation and characterization of a conditional eNOS knock out mouse model for cell-specific reactivation of eNOS in gain-of-function studies
Generation and characterization of a conditional eNOS knock out mouse model for cell-specific reactivation of eNOS in gain-of-function studies Open
Nitric oxide (NO) produced by endothelial nitric oxide synthase (eNOS) in the vessel wall regulates blood pressure and cardiovascular hemodynamics. In this study, we generated conditional eNOS knock out (KO) mice characterized by a duplica…
View article: Nitrosation of CD36 regulates endothelial function and serum lipids
Nitrosation of CD36 regulates endothelial function and serum lipids Open
During obesity, endothelial cells (ECs) become lipid laden leading to endothelial dysfunction. We demonstrate endothelium downregulates caveolin-1 (Cav1) in mouse and human in response to obesity. Using an EC-specific Cav1 knockout mouse, …
View article: Heme metabolism in nonerythroid cells
Heme metabolism in nonerythroid cells Open
View article: Endothelial-adipocyte Cx43 Mediated Gap Junctions Can Regulate Adiposity
Endothelial-adipocyte Cx43 Mediated Gap Junctions Can Regulate Adiposity Open
Obesity is a multifactorial metabolic disorder associated with endothelial dysfunction and increased risk of cardiovascular disease. Adipose capillary adipose endothelial cells (CaECs) plays a crucial role in lipid transport and storage. H…
View article: Obesogenic diet disrupts tissue-specific mitochondrial gene signatures in the artery and capillary endothelium
Obesogenic diet disrupts tissue-specific mitochondrial gene signatures in the artery and capillary endothelium Open
Using bulk and single-cell RNA sequencing on endothelial cells from adipose and mesentery, we found that an obesogenic diet induces a reduction in adipose endothelial oxidative phosphorylation gene expression, resulting in a phenotypic con…
View article: Loss of endothelial cell heterogeneity in arteries after obesogenic diet
Loss of endothelial cell heterogeneity in arteries after obesogenic diet Open
Background It is well recognized that obesity leads to arterial endothelial dysfunction and cardiovascular disease. However, the progression to endothelial dysfunction is not clear. Endothelial cells (ECs) adapt to the unique needs of thei…
View article: Endothelial Histone Deacetylase 1 Activity Impairs Kidney Microvascular NO Signaling in Rats fed a High Salt Diet
Endothelial Histone Deacetylase 1 Activity Impairs Kidney Microvascular NO Signaling in Rats fed a High Salt Diet Open
Aim We aimed to identify new mechanisms by which a high salt diet (HS) decreases NO production in kidney microvascular endothelial cells. Specifically, we hypothesized HS impairs NO signaling through a histone deacetylase 1 (HDAC1)-depende…
View article: Amount of Pannexin 1 in Smooth Muscle Cells Regulates Sympathetic Nerve–Induced Vasoconstriction
Amount of Pannexin 1 in Smooth Muscle Cells Regulates Sympathetic Nerve–Induced Vasoconstriction Open
Background: Panx1 (pannexin 1) forms high conductance channels that secrete ATP upon stimulation. The role of Panx1 in mediating constriction in response to direct sympathetic nerve stimulation is not known. Additionally, it is unknown how…
View article: Amount of Pannexin 1 in smooth muscle cells regulates sympathetic nerve induced vasoconstriction
Amount of Pannexin 1 in smooth muscle cells regulates sympathetic nerve induced vasoconstriction Open
Pannexin 1 (Panx1) forms high conductance channels that secrete ATP upon stimulation. The role of Panx1 in mediating constriction in response to direct sympathetic nerve stimulation is not known. Additionally, it is unknown how the express…
View article: HDAC1: an environmental sensor regulating endothelial function
HDAC1: an environmental sensor regulating endothelial function Open
The histone deacetylases (HDACs) are a family of enzymes that catalyse lysine deacetylation of both histone and non-histone proteins. Here, we review, summarize, and provide perspectives on the literature regarding one such HDAC, HDAC1, in…
View article: Hydroxyurea improves nitric oxide bioavailability in humanized sickle cell mice
Hydroxyurea improves nitric oxide bioavailability in humanized sickle cell mice Open
Despite advancements in disease management, sickle cell nephropathy, a major contributor to mortality and morbidity in patients, has limited therapeutic options. Previous studies indicate hydroxyurea, a commonly prescribed therapy for sick…