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View article: Cosolvent-induced Spontaneous Refolding of Lipase
Cosolvent-induced Spontaneous Refolding of Lipase Open
The overexpression of proteins in Escherichia coli often results in the formation of inclusion bodies, which are biologically inactive, especially for proteins with exposed hydrophobic surfaces. Solubilization of IBs and subsequent refoldi…
View article: Fluorescent Labeling of Dicysteine-Tagged Peptide for Monitoring and Optimization of Protein Bio-production in Bacteria
Fluorescent Labeling of Dicysteine-Tagged Peptide for Monitoring and Optimization of Protein Bio-production in Bacteria Open
A rapid and convenient strategy to monitor the productivity of biomanufacturing is essential for the research in optimizing relevant bioprocesses. In this work, we have developed a fluorescein-derived probe (FL-DT) that reacts rapidly with…
View article: A novel Ffu fusion system for secretory expression of heterologous proteins in Escherichia coli
A novel Ffu fusion system for secretory expression of heterologous proteins in Escherichia coli Open