Luyan Cao
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View article: Arp2/3-mediated bidirectional actin assembly by SPIN90 dimers in metazoans
Arp2/3-mediated bidirectional actin assembly by SPIN90 dimers in metazoans Open
Branched actin networks nucleated by the Arp2/3 complex play critical roles in various cellular processes, from cell migration to intracellular transport. However, when activated by WISH/DIP/SPIN90 family proteins, Arp2/3 nucleates linear …
View article: The stabilization of Arp2/3 complex generated actin filaments
The stabilization of Arp2/3 complex generated actin filaments Open
The Arp2/3 complex, which generates both branched but also linear actin filaments via activation of SPIN90, is evolutionarily conserved in eukaryotes. Several factors regulate the stability of filaments generated by the Arp2/3 complex to m…
View article: Regeneration of actin filament branches from the same Arp2/3 complex
Regeneration of actin filament branches from the same Arp2/3 complex Open
Branched actin filaments are found in many key cellular structures. Branches are nucleated by the Arp2/3 complex activated by nucleation-promoting factor (NPF) proteins and bound to the side of preexisting “mother” filaments. Over time, br…
View article: CK-666 and CK-869 differentially inhibit Arp2/3 iso-complexes
CK-666 and CK-869 differentially inhibit Arp2/3 iso-complexes Open
The inhibitors, CK-666 and CK-869, are widely used to probe the function of actin nucleation by the Arp2/3 complex in vitro and in cells. However, in mammals, the Arp2/3 complex consists of 8 iso-complexes, as three of its subunits (Arp3, …
View article: Regeneration of actin filament branches from the same Arp2/3 complex
Regeneration of actin filament branches from the same Arp2/3 complex Open
Branched actin filaments are found in many key cellular structures. Branches are nucleated by the Arp2/3 complex activated by nucleation-promoting factor (NPF) proteins and bound to the side of pre-existing ‘mother’ filaments. Over time, b…
View article: Cortactin stabilizes actin branches by bridging activated Arp2/3 to its nucleated actin filament
Cortactin stabilizes actin branches by bridging activated Arp2/3 to its nucleated actin filament Open
SUMMARY Regulation of the assembly and turnover of branched actin filament networks nucleated by the Arp2/3 complex is essential during many cellular processes including cell migration and membrane trafficking. Cortactin plays a key role i…
View article: Direct observation of the conformational states of formin mDia1 at actin filament barbed ends and along the filament
Direct observation of the conformational states of formin mDia1 at actin filament barbed ends and along the filament Open
Using electron microscopy, two conformations of the mDia1 formin FH2 domains were directly identified in interaction with the barbed ends of actin. These conformations agree with the speculated open and closed conformations of the “stair-s…
View article: Direct observation of the conformational states of formin mDia1 at actin filament barbed ends and along the filament
Direct observation of the conformational states of formin mDia1 at actin filament barbed ends and along the filament Open
The fine regulation of actin polymerization is essential to control cell motility, architecture and to perform essential cellular functions. Formins are key regulators of actin filament assembly, known to processively elongate filament bar…
View article: Nucleation and stability of branched versus linear Arp2/3-generated actin filaments
Nucleation and stability of branched versus linear Arp2/3-generated actin filaments Open
Activation of the Arp2/3 complex by VCA-domain-bearing NPFs results in the formation of ‘daughter’ actin filaments branching off the sides of pre-existing ‘mother filaments.’ Alternatively, when stimulated by SPIN90, Arp2/3 directly nuclea…
View article: The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex
The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex Open
Dendritic actin networks grow in an autocatalytic manner starting from the uncapped minifilament of dynactin.
View article: The Arp1/11 Minifilament of Dynactin Primes the Endosomal Arp2/3 Complex
The Arp1/11 Minifilament of Dynactin Primes the Endosomal Arp2/3 Complex Open
Dendritic actin networks develop from a first actin filament through branching by the Arp2/3 complex. At the surface of endosomes, the WASH complex activates the Arp2/3 complex and interacts with the Capping Protein for unclear reasons. He…
View article: Cryo-EM of human Arp2/3 complexes provides structural insights into actin nucleation modulation by ARPC5 isoforms
Cryo-EM of human Arp2/3 complexes provides structural insights into actin nucleation modulation by ARPC5 isoforms Open
The Arp2/3 complex regulates many cellular processes by stimulating formation of branched actin filament networks. Because three of its seven subunits exist as two different isoforms, mammals produce a family of Arp2/3 complexes with diffe…
View article: Cryo-EM of human Arp2/3 complexes provides structural insights into actin nucleation modulation by ARPC5 isoforms
Cryo-EM of human Arp2/3 complexes provides structural insights into actin nucleation modulation by ARPC5 isoforms Open
The Arp2/3 complex regulates many cellular processes by stimulating formation of branched actin filament networks. Because three of its seven subunits exist as two different isoforms, mammals produce a family of Arp2/3 complexes with diffe…
View article: The advantages of microfluidics to study actin biochemistry and biomechanics
The advantages of microfluidics to study actin biochemistry and biomechanics Open
The regulated assembly of actin filaments is essential in nearly all cell types. Studying actin assembly dynamics can pose many technical challenges. A number of these challenges can be overcome by using microfluidics to observe and manipu…
View article: Modulation of formin processivity by profilin and mechanical tension
Modulation of formin processivity by profilin and mechanical tension Open
Formins are major regulators of actin networks. They enhance actin filament dynamics by remaining processively bound to filament barbed ends. How biochemical and mechanical factors affect formin processivity are open questions. Monitoring …
View article: Modulation of formin processivity by profilin and mechanical tension
Modulation of formin processivity by profilin and mechanical tension Open
Formins are major regulators of actin networks. They enhance actin filament dynamics by remaining processively bound to filament barbed ends. How biochemical and mechanical factors affect formin processivity are open questions. Monitoring …