Explanipedia

High-throughput Single-Cell Proteomics and Transcriptomics from the Same Cells with a Nanoliter-Scale Spin-Transfer Approach Open

Pranav Dawar, Lye Meng Markillie, Sarah Williams, Hugh Mitchell, Johannes Bagnoli , et al. · 2025

Single-cell multiomic platforms provide a comprehensive snapshot of cellular states and cell types by offering critical insights into the spatiotemporal regulation of biomolecular networks at a systems level, thereby defining the basis of …

Insulin post-transcriptional regulation via PARP12-mediated ADP- ribosylation Open

Soumyadeep Sarkar, Fangjia Li, Youngki You, Emily C. Elliott, Kevin Zemaitis , et al. · 2025

The role of Ca-bridged organic matter in an alkaline soil, as revealed by multimodal chemical imaging Open

Tamás Varga, Ravi Kukkadapu, Libor Kovařík, Alice Dohnálková, Qian Zhao , et al. · 2025

Mineral–organic matter (OM) studies have predominantly focused on acidic soils that are abundant in iron (Fe) oxides and aluminum (Al) oxides. We have probed mineral–OM interactions in an alkaline or calcareous soil of the Aridisols class.…

Metabolic profiling of two white-rot fungi during 4-hydroxybenzoate conversion reveals biotechnologically relevant biosynthetic pathways Open

Lummy Maria Oliveira Monteiro, Carlos del Cerro, Teeratas Kijpornyongpan, Allison Yaguchi, Anna K. Bennett , et al. · 2025

White-rot fungi are efficient organisms for the mineralization of lignin and polysaccharides into CO₂ and H₂O. Despite their biotechnological potential, WRF metabolism remains underexplored. Building on recent finding…

Data for EMSL Project 60578 from December 2023 Open

Scott Baker, Nikola Tolić, William Chrisler, Lye Meng Markillie, Hugh Mitchell , et al. · 2025

Metabolic engineering reveals the relative importance of different sugar catabolic pathways during consumption of plant biomass by Aspergillus niger Open

Tania Chroumpi, Astrid Müller, Mao Peng, M. Navarro, Robert W. A. M. Kuijpers , et al. · 2025

To evaluate the impact of individual sugar catabolic pathways on the physiology of A. niger when using plant biomass as a carbon source, key pathways converting plant biomass derived monomers were blocked. The resulting deletion mutants we…

Parallel measurement of transcriptomes and proteomes from same single cells using nanodroplet splitting Open

James Fulcher, Lye Meng Markillie, Hugh Mitchell, Sarah Williams, Kristin Engbrecht , et al. · 2024

Single-cell multiomics provides comprehensive insights into gene regulatory networks, cellular diversity, and temporal dynamics. Here, we introduce nanoSPLITS (nanodroplet SPlitting for Linked-multimodal Investigations of Trace Samples), a…

Device and Method for Parallel Measurement of Phosphoproteome and Proteome from Single Cells Open

Liang Chen, Lye Meng Markillie · 2024

We present the development of an immobilized metal affinity chromatography (IMAC) chip designed to enable nanoscale phosphopeptide enrichment within microfabricated nanowells. This novel platform leverages surface chemistry to immobilize h…

Proteome-Scale Tissue Mapping Using Mass Spectrometry Based on Label-Free and Multiplexed Workflows Open

Yumi Kwon, Jongmin Jacob Woo, Fengchao Yu, Sarah Williams, Lye Meng Markillie , et al. · 2024

Multiplexed bimolecular profiling of tissue microenvironment, or spatial omics, can provide deep insight into cellular compositions and interactions in healthy and diseased tissues. Proteome-scale tissue mapping, which aims to unbiasedly v…

Proteome-scale tissue mapping using mass spectrometry based on label-free and multiplexed workflows Open

Yumi Kwon, Jongmin Jacob Woo, Fengchao Yu, Sarah Williams, Lye Meng Markillie , et al. · 2024

Multiplexed bimolecular profiling of tissue microenvironment, or spatial omics, can provide deep insight into cellular compositions and interactions in healthy and diseased tissues. Proteome-scale tissue mapping, which aims to unbiasedly v…

Cell‐type‐specific transcriptomics uncovers spatial regulatory networks in bioenergy sorghum stems Open

Jie Fu, Brian McKinley, Brandon James, William Chrisler, Lye Meng Markillie , et al. · 2024

SUMMARY Bioenergy sorghum is a low‐input, drought‐resilient, deep‐rooting annual crop that has high biomass yield potential enabling the sustainable production of biofuels, biopower, and bioproducts. Bioenergy sorghum's 4–5 m stems account…

Novel synthetic inducible promoters controlling gene expression during water‐deficit stress with green tissue specificity in transgenic poplar Open

Yongil Yang, Timothy A. Chaffin, Yuanhua Shao, Vimal Kumar Balasubramanian, Lye Meng Markillie , et al. · 2024

Summary Synthetic promoters may be designed using short cis ‐regulatory elements (CREs) and core promoter sequences for specific purposes. We identified novel conserved DNA motifs from the promoter sequences of leaf palisade and vascular c…

Data for EMSL Project 51636 from March 2024 Open

Mary Lipton, Galya Orr, Scott Baker, Ziyu Dai, Alex Beliaev , et al. · 2024

Data for EMSL Project 51636 from March 2024 Open

Mary Lipton, Galya Orr, Scott Baker, Ziyu Dai, Alex Beliaev , et al. · 2024

Data for EMSL Project 51636 from March 2024 Open

Mary Lipton, Galya Orr, Scott Baker, Ziyu Dai, Alex Beliaev , et al. · 2024

Data for EMSL Project 60582 from February 2024 Open

Alice Dohnálková, John Cliff, William Chrisler, Tamás Varga, Lye Meng Markillie , et al. · 2024

Data for EMSL Project 51636 from January 2024 Open

Mary Lipton, Galya Orr, Galya Orr, Scott Baker, Ziyu Dai , et al. · 2024

Data for EMSL Project 51636 from February 2024 Open

Mary Lipton, Galya Orr, Galya Orr, Scott Baker, Ziyu Dai , et al. · 2024

Data for EMSL Project 51636 from February 2024 Open

Mary Lipton, Galya Orr, Galya Orr, Scott Baker, Ziyu Dai , et al. · 2024

Data for EMSL Project 51636 from February 2024 Open

Mary Lipton, Galya Orr, Galya Orr, Scott Baker, Ziyu Dai , et al. · 2024

Data for EMSL Project 51636 from March 2024 Open

Mary Lipton, Galya Orr, Scott Baker, Ziyu Dai, Alex Beliaev , et al. · 2024

Data for EMSL Project 60578 from January 2024 Open

Scott Baker, Nikola Tolić, William Chrisler, Lye Meng Markillie, Hugh Mitchell , et al. · 2024

Data for EMSL Project 51636 from February 2024 Open

Mary Lipton, Galya Orr, Galya Orr, Scott Baker, Ziyu Dai , et al. · 2024

Data for EMSL Project 51636 from March 2024 Open

Mary Lipton, Galya Orr, Scott Baker, Ziyu Dai, Alex Beliaev , et al. · 2024

Data for EMSL Project 51636 from February 2024 Open

Mary Lipton, Galya Orr, Galya Orr, Scott Baker, Ziyu Dai , et al. · 2024

Data for EMSL Project 51636 from March 2024 Open

Mary Lipton, Galya Orr, Scott Baker, Ziyu Dai, Alex Beliaev , et al. · 2024

Data for EMSL Project 60582 from February 2024 Open

Alice Dohnálková, John Cliff, William Chrisler, Tamás Varga, Lye Meng Markillie , et al. · 2024

Data for EMSL Project 51636 from March 2024 Open

Mary Lipton, Galya Orr, Scott Baker, Ziyu Dai, Alex Beliaev , et al. · 2024

Data for EMSL Project 51636 from March 2024 Open

Mary Lipton, Galya Orr, Scott Baker, Ziyu Dai, Alex Beliaev , et al. · 2024

Data for EMSL Project 51636 from March 2024 Open

Mary Lipton, Galya Orr, Scott Baker, Ziyu Dai, Alex Beliaev , et al. · 2024

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