M. Atger
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View article: Autoantibodies interacting with purified native thyrotropin receptor
Autoantibodies interacting with purified native thyrotropin receptor Open
Native thyrotropin receptor (TSHR) was purified by immunoaffinity chromatography from membrane extracts of stably transfected L cells. An ELISA test was devised to study anti‐TSHR autoantibodies directly. Comparison of native TSHR with bac…
View article: Organisation of the entire rabbit progesterone receptor mRNA and of the promoter and 5′ flanking region of the gene
Organisation of the entire rabbit progesterone receptor mRNA and of the promoter and 5′ flanking region of the gene Open
cDNA clones corresponding to the 3' and 5' non coding regions of the rabbit progesterone receptor (rPR) mRNA and genomic clones corresponding to the promoter and 5' flanking region of this gene were isolated and sequenced up to nucleotide …
View article: Cloning and sequence analysis of rabbit progesterone-receptor complementary DNA.
Cloning and sequence analysis of rabbit progesterone-receptor complementary DNA. Open
Two lambda gt11 clones containing fragments of cDNA encoding the rabbit progesterone receptor were isolated with the aid of monoclonal and monospecific polyclonal antireceptor antibodies. RNA gel blot analysis showed that the corresponding…
View article: The rabbit uteroglobin gene. Structure and interaction with the progesterone receptor.
The rabbit uteroglobin gene. Structure and interaction with the progesterone receptor. Open
The study of the regulation of uteroglobin gene in the rabbit endometrium constitutes a model for analyzing the mechanism of action of progesterone in mammals. The gene has been cloned into lambda phage and sequenced. Comparison of the seq…
View article: Cloning of rabbit genomic fragments containing the uteroglobin gene.
Cloning of rabbit genomic fragments containing the uteroglobin gene. Open
Hybrid phages containing the uteroglobin gene were isolated from a library of Charon 4A lambda phages carrying fragments of rabbit genome. Two phages which contain overlapping rabbit DNA covering approximately 35 kilobase pairs (kb) on bot…
View article: <i>N</i>-terminal sequences of uteroglobin and its precursor
<i>N</i>-terminal sequences of uteroglobin and its precursor Open
Translation of uteroglobin mRNA in wheat-germ extract has yielded a precursor protein (pre-uteroglobin) containing an N-terminal extension of 21 amino acid residues. The sequence of this extension and that of the 50 N-terminal amino acid r…
View article: Mechanism and kinetics of the thermal activation of glucocorticoid hormone receptor complex.
Mechanism and kinetics of the thermal activation of glucocorticoid hormone receptor complex. Open
Steroid-receptor complexes formed at low temperature and ionic strength do not bind to nuclei or chromatin. After a temporary exposure to high temperature, or ionic strength, or both, a fraction of them becomes activated (able to bind to n…
View article: Mechanisms Regulating the Concentration and the Conformation of Progesterone Receptor(s) in the Uterus
Mechanisms Regulating the Concentration and the Conformation of Progesterone Receptor(s) in the Uterus Open
After administration of estradiol to castrated guinea pigs, a rise in the uterine concentration of specific progesterone binding sites ("receptor") was observed as early as at 6 hours, and the peak of concentration (8-fold over basal value…
View article: Progesterone-binding Plasma Protein of Pregnant Guinea Pig
Progesterone-binding Plasma Protein of Pregnant Guinea Pig Open
The progesterone-binding plasma protein (PBP) of the pregnant guinea pig has been purified to homogeneity. The molecular weight, 77,500 (as determined by equilibrium sedimentation and sodium dodecyl sulfate polyacrylamide gel electrophores…
View article: Progesterone in Uterus and Plasma: VI. Uterine Progesterone Receptors during the Estrus Cycle and Implantation in the Guinea Pig
Progesterone in Uterus and Plasma: VI. Uterine Progesterone Receptors during the Estrus Cycle and Implantation in the Guinea Pig Open
The concentration of the progesterone cytosol receptor in the uterus shows marked variations during the estrus cycle. It is maximum in proestrus (about 40,000 binding sites per cell) and falls progressively during estrus and postestrus to …