M. El Bakkouri
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View article: Broadly reactive anti-VHH antibodies for characterizing, blocking, or activating nanobody-based CAR-T cells
Broadly reactive anti-VHH antibodies for characterizing, blocking, or activating nanobody-based CAR-T cells Open
Background Production of chimeric antigen receptor T cell (CAR-T) therapies depends on antibody reagents to label, isolate, and expand T cell products. We sought to create antibody tools specific for the variable domain of heavy-chain only…
View article: Broadly Reactive Anti-VHH Antibodies for Characterizing, Blocking, or Activating Nanobody-Based CAR-T Cells
Broadly Reactive Anti-VHH Antibodies for Characterizing, Blocking, or Activating Nanobody-Based CAR-T Cells Open
Production of chimeric antigen receptor T cell (CAR-T) therapies is dependent on the use of antibody reagents to label, isolate, and/or expand T cell products. We sought to create antibody-based tools that directly target the variable doma…
View article: POTRA domains of the TamA insertase interact with the outer membrane and modulate membrane properties
POTRA domains of the TamA insertase interact with the outer membrane and modulate membrane properties Open
The outer membrane (OM) of gram-negative bacteria serves as a vital organelle that is densely populated with OM proteins (OMPs) and plays pivotal roles in cellular functions and virulence. The assembly and insertion of these OMPs into the …
View article: Unraveling the crystal structure of the HpaA adhesin: insights into cell adhesion function and epitope localization of a <i>Helicobacter pylori</i> vaccine candidate
Unraveling the crystal structure of the HpaA adhesin: insights into cell adhesion function and epitope localization of a <i>Helicobacter pylori</i> vaccine candidate Open
Helicobacter pylori is a bacterium that exhibits strict host restriction to humans and non-human primates, and the bacterium is widely acknowledged as a significant etiological factor in the development of chronic gastritis, peptic ulcers,…
View article: Using a Genetically Encoded Sensor to Identify Inhibitors of Toxoplasma gondii Ca 2+ Signaling
Using a Genetically Encoded Sensor to Identify Inhibitors of Toxoplasma gondii Ca 2+ Signaling Open
The life cycles of apicomplexan parasites progress in accordance with fluxes in cytosolic Ca2+. Such fluxes are necessary for events like motility and egress from host cells. We used genetically encoded Ca2+ indicators (GCaMPs) to develop …
View article: Structures of the cGMP-dependent protein kinase in malaria parasites reveal a unique structural relay mechanism for activation
Structures of the cGMP-dependent protein kinase in malaria parasites reveal a unique structural relay mechanism for activation Open
The cyclic guanosine-3′,5′-monophosphate (cGMP)-dependent protein kinase (PKG) was identified >25 y ago; however, efforts to obtain a structure of the entire PKG enzyme or catalytic domain from any species have failed. In malaria parasites…
View article: Inhibition of Calcium Dependent Protein Kinase 1 (CDPK1) by Pyrazolopyrimidine Analogs Decreases Establishment and Reoccurrence of Central Nervous System Disease by <i>Toxoplasma gondii</i>
Inhibition of Calcium Dependent Protein Kinase 1 (CDPK1) by Pyrazolopyrimidine Analogs Decreases Establishment and Reoccurrence of Central Nervous System Disease by <i>Toxoplasma gondii</i> Open
Calcium dependent protein kinase 1 (CDPK1) is an essential enzyme in the opportunistic pathogen Toxoplasma gondii. CDPK1 controls multiple processes that are critical to the intracellular replicative cycle of T. gondii including secretion …
View article: Biochemical Screening of Five Protein Kinases from Plasmodium falciparum against 14,000 Cell-Active Compounds
Biochemical Screening of Five Protein Kinases from Plasmodium falciparum against 14,000 Cell-Active Compounds Open
In 2010 the identities of thousands of anti-Plasmodium compounds were released publicly to facilitate malaria drug development. Understanding these compounds' mechanisms of action--i.e., the specific molecular targets by which they kill th…
View article: Using a Genetically Encoded Sensor to Identify Inhibitors of Toxoplasma gondii Ca2+ Signaling
Using a Genetically Encoded Sensor to Identify Inhibitors of Toxoplasma gondii Ca2+ Signaling Open
The life cycles of apicomplexan parasites progress in accordance with fluxes in cytosolic Ca(2+) Such fluxes are necessary for events like motility and egress from host cells. We used genetically encoded Ca(2+) indicators (GCaMPs) to devel…
View article: The molecular mechanism of Zinc acquisition by the neisserial outer-membrane transporter ZnuD
The molecular mechanism of Zinc acquisition by the neisserial outer-membrane transporter ZnuD Open
Invading bacteria from the Neisseriaceae , Acinetobacteriaceae , Bordetellaceae and Moraxellaceae families express the conserved outer-membrane zinc transporter zinc-uptake component D (ZnuD) to overcome nutritional restriction imposed by …