Nelia M. Gerez de Burgos
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View article: Daily Variations in the Glycerol-3-Phosphate Dehydrogenase Isoforms Expression in Triatoma infestans Flight Muscles
Daily Variations in the Glycerol-3-Phosphate Dehydrogenase Isoforms Expression in Triatoma infestans Flight Muscles Open
Triatoma infestans , the main vector of Chagas disease, is a blood-sucking insect. Flight dispersal of adults is the most important mechanism for reinfestation of houses after insecticide spraying. Flight muscles have two glycerol-3-phosph…
View article: Differential Expression of Glycerol-3-Phosphate Dehydrogenase Isoforms in Flight Muscles of the Chagas Disease Vector Triatoma infestans (Hemiptera, Reduviidae)
Differential Expression of Glycerol-3-Phosphate Dehydrogenase Isoforms in Flight Muscles of the Chagas Disease Vector Triatoma infestans (Hemiptera, Reduviidae) Open
Flight muscles of Triatoma infestans have two glycerol-3-phosphate dehydrogenase (GPDH) isoforms: GPDH-1 is involved in flight metabolism and GPDH-2 provides lipid precursors. Total GPDH activity was greater in the natural population and a…
View article: Gene discovery in Triatoma infestans
Gene discovery in Triatoma infestans Open
Background Triatoma infestans is the most relevant vector of Chagas disease in the southern cone of South America. Since its genome has not yet been studied, sequencing of Expressed Sequence Tags (ESTs) is one of the most powerful tools fo…
View article: Differential Tissue and Flight Developmental Expression of Glycerol-3-Phosphate Dehydrogenase Isozymes in the Chagas Disease Vector Triatoma infestans
Differential Tissue and Flight Developmental Expression of Glycerol-3-Phosphate Dehydrogenase Isozymes in the Chagas Disease Vector Triatoma infestans Open
Glycerol-3-phosphate dehydrogenase (GPDH) isozymes are differentially expressed among tissues and during flight development. GPDH-1 is involved in the flight-muscle metabolism and GPDH-2 provides precursors for lipid biosynthesis in many t…
View article: Arginine kinase of the flagellate protozoa <i>Trypanosoma cruzi</i>
Arginine kinase of the flagellate protozoa <i>Trypanosoma cruzi</i> Open
In epimastigotes of Trypanosoma cruzi , the etiological agent of Chagas’ disease, arginine kinase activity increased continuously during the exponential phase of growth. A correlation between growth rate, enzyme‐specific activity and enzym…
View article: Intracellular Localization of the Testicular and Sperm-Specific Lactate Dehydrogenase Isozyme C4 in Mice1
Intracellular Localization of the Testicular and Sperm-Specific Lactate Dehydrogenase Isozyme C4 in Mice1 Open
The proposed dual intracellular distribution of the sperm-specific lactate dehydrogenase (EC 1.1.1.27) isozyme C4 (LDH C4) has been based on indirect evidence. In order to obtain direct evidence on the localization of this LDH isozyme in m…
View article: Properties of the branched-chain 2-hydroxy acid/2-oxo acid shuttle in mouse spermatozoa
Properties of the branched-chain 2-hydroxy acid/2-oxo acid shuttle in mouse spermatozoa Open
Operation of the branched-chain 2-hydroxy acid/2-oxo acid shuttle for the transfer of reducing equivalents in mitochondria of mouse spermatozoa was studied in vitro in reconstituted systems. Results show that the branched-chain 2-oxo acids…
View article: Studies <i>in vitro</i> on shuttle systems of mouse spermatozoa
Studies <i>in vitro</i> on shuttle systems of mouse spermatozoa Open
Observations on systems reconstituted in vitro with different starting substrates (2-hydroxy-acids, 2-oxo-acids or leucine) indicate that a branched-chain 2-hydroxy-acid/2-oxo-acid shuttle for the transfer of reducing equivalents from cyto…
View article: Correlation of lactate dehydrogenase isoenzyme C4 activity with the count and motility of human spermatozoa
Correlation of lactate dehydrogenase isoenzyme C4 activity with the count and motility of human spermatozoa Open
The activity of lactate dehydrogenase isoenzyme C4 was determined on 90 human semen samples. The correlation between the isoenzyme activity and sperm count and motility was good (r = 0.74 for values of U/ml semen against sperm count).
View article: Substrate specificity of the lactate dehydrogenase isoenzyme C4 from human spermatozoa and a possible selective assay
Substrate specificity of the lactate dehydrogenase isoenzyme C4 from human spermatozoa and a possible selective assay Open
The activity of lactate dehydrogenase (EC 1.1.1.27) in normal human sperm lysates and in human heart and liver homogenates was determined by using a variety of 2-oxoacids as substrates. Sperm preparations were active with pyruvate, 2-oxobu…
View article: Properties of the testicular lactate dehydrogenase isoenzyme
Properties of the testicular lactate dehydrogenase isoenzyme Open
1. Studies were carried out with pure lactate dehydrogenase isoenzymes C4 (LDH isoenzyme X), B4, (LDH isoenzyme 1) and A4 (LDH isoenzyme 5) isolated from mouse testis, heart and muscle tissue respectively; with LDH isoenzyme X purified fro…